Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS15995 and ABIE41_RS16000 are separated by 170 nucleotides ABIE41_RS16000 and ABIE41_RS16005 are separated by 128 nucleotides ABIE41_RS16005 and ABIE41_RS16010 are separated by 99 nucleotides ABIE41_RS16010 and ABIE41_RS16015 are separated by 98 nucleotides
ABIE41_RS15995: ABIE41_RS15995 - RsmB/NOP family class I SAM-dependent RNA methyltransferase, at 3,278,412 to 3,279,710
_RS15995
ABIE41_RS16000: ABIE41_RS16000 - hypothetical protein, at 3,279,881 to 3,280,024
_RS16000
ABIE41_RS16005: ABIE41_RS16005 - hypothetical protein, at 3,280,153 to 3,280,422
_RS16005
ABIE41_RS16010: ABIE41_RS16010 - amino acid ABC transporter ATP-binding protein, at 3,280,522 to 3,281,238
_RS16010
ABIE41_RS16015: ABIE41_RS16015 - amino acid ABC transporter permease, at 3,281,337 to 3,282,716
_RS16015
Position (kb) 3280
3281 Strain fitness (log2 ratio) -1
0
1
2 at 3279.560 kb on - strand, within ABIE41_RS15995 at 3280.054 kb on - strand at 3280.368 kb on - strand, within ABIE41_RS16005 at 3280.850 kb on + strand, within ABIE41_RS16010 at 3280.850 kb on + strand, within ABIE41_RS16010 at 3280.890 kb on - strand, within ABIE41_RS16010 at 3281.216 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM remove 3,279,560 - ABIE41_RS15995 0.88 -1.6 3,280,054 - -1.2 3,280,368 - ABIE41_RS16005 0.80 +1.8 3,280,850 + ABIE41_RS16010 0.46 +0.2 3,280,850 + ABIE41_RS16010 0.46 +0.8 3,280,890 - ABIE41_RS16010 0.51 -0.4 3,281,216 + -1.4
Or see this region's nucleotide sequence