Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS14175 and ABIE41_RS14180 are separated by 346 nucleotides ABIE41_RS14180 and ABIE41_RS14185 are separated by 12 nucleotides ABIE41_RS14185 and ABIE41_RS14190 are separated by 214 nucleotides ABIE41_RS14190 and ABIE41_RS14195 are separated by 236 nucleotides
ABIE41_RS14175: ABIE41_RS14175 - MFS transporter, at 2,902,674 to 2,904,299
_RS14175
ABIE41_RS14180: ABIE41_RS14180 - hypothetical protein, at 2,904,646 to 2,905,170
_RS14180
ABIE41_RS14185: ABIE41_RS14185 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB, at 2,905,183 to 2,906,028
_RS14185
ABIE41_RS14190: ABIE41_RS14190 - tRNA-Tyr, at 2,906,243 to 2,906,327
_RS14190
ABIE41_RS14195: ABIE41_RS14195 - hypothetical protein, at 2,906,564 to 2,907,070
_RS14195
Position (kb)
2905
2906
2907 Strain fitness (log2 ratio)
-1
0
1 at 2904.230 kb on - strand at 2904.230 kb on - strand at 2904.355 kb on - strand at 2904.355 kb on - strand at 2904.527 kb on + strand at 2904.528 kb on - strand at 2904.528 kb on - strand at 2904.528 kb on - strand at 2904.538 kb on - strand at 2904.599 kb on + strand at 2904.599 kb on + strand at 2904.599 kb on + strand at 2904.599 kb on + strand at 2904.600 kb on - strand at 2904.600 kb on - strand at 2904.600 kb on - strand at 2904.981 kb on + strand, within ABIE41_RS14180 at 2904.981 kb on + strand, within ABIE41_RS14180 at 2904.981 kb on + strand, within ABIE41_RS14180 at 2904.981 kb on + strand, within ABIE41_RS14180 at 2904.982 kb on - strand, within ABIE41_RS14180 at 2904.982 kb on - strand, within ABIE41_RS14180 at 2905.034 kb on - strand, within ABIE41_RS14180 at 2905.077 kb on - strand, within ABIE41_RS14180 at 2905.077 kb on - strand, within ABIE41_RS14180 at 2905.077 kb on - strand, within ABIE41_RS14180 at 2905.205 kb on + strand at 2905.205 kb on + strand at 2905.683 kb on - strand, within ABIE41_RS14185 at 2905.683 kb on - strand, within ABIE41_RS14185 at 2905.683 kb on - strand, within ABIE41_RS14185 at 2905.683 kb on - strand, within ABIE41_RS14185 at 2906.511 kb on + strand at 2906.664 kb on + strand, within ABIE41_RS14195 at 2906.665 kb on - strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.828 kb on + strand, within ABIE41_RS14195 at 2906.829 kb on - strand, within ABIE41_RS14195 at 2906.829 kb on - strand, within ABIE41_RS14195
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM remove 2,904,230 - -0.5 2,904,230 - -0.4 2,904,355 - -0.8 2,904,355 - -0.7 2,904,527 + +0.5 2,904,528 - +0.1 2,904,528 - -0.6 2,904,528 - +0.4 2,904,538 - +0.3 2,904,599 + -0.8 2,904,599 + -0.1 2,904,599 + -0.1 2,904,599 + +0.1 2,904,600 - +0.3 2,904,600 - -0.2 2,904,600 - -0.3 2,904,981 + ABIE41_RS14180 0.64 -1.1 2,904,981 + ABIE41_RS14180 0.64 -0.5 2,904,981 + ABIE41_RS14180 0.64 -0.8 2,904,981 + ABIE41_RS14180 0.64 +0.5 2,904,982 - ABIE41_RS14180 0.64 +0.8 2,904,982 - ABIE41_RS14180 0.64 -1.6 2,905,034 - ABIE41_RS14180 0.74 -1.3 2,905,077 - ABIE41_RS14180 0.82 +0.5 2,905,077 - ABIE41_RS14180 0.82 +0.7 2,905,077 - ABIE41_RS14180 0.82 -1.0 2,905,205 + -0.2 2,905,205 + +0.4 2,905,683 - ABIE41_RS14185 0.59 -0.1 2,905,683 - ABIE41_RS14185 0.59 -0.1 2,905,683 - ABIE41_RS14185 0.59 -0.6 2,905,683 - ABIE41_RS14185 0.59 -0.7 2,906,511 + -1.7 2,906,664 + ABIE41_RS14195 0.20 +0.3 2,906,665 - ABIE41_RS14195 0.20 -0.5 2,906,828 + ABIE41_RS14195 0.52 +0.8 2,906,828 + ABIE41_RS14195 0.52 -0.1 2,906,828 + ABIE41_RS14195 0.52 -0.5 2,906,828 + ABIE41_RS14195 0.52 +0.6 2,906,828 + ABIE41_RS14195 0.52 +0.2 2,906,828 + ABIE41_RS14195 0.52 -0.4 2,906,828 + ABIE41_RS14195 0.52 +0.2 2,906,828 + ABIE41_RS14195 0.52 +0.1 2,906,828 + ABIE41_RS14195 0.52 +0.0 2,906,829 - ABIE41_RS14195 0.52 +0.3 2,906,829 - ABIE41_RS14195 0.52 +1.5
Or see this region's nucleotide sequence