Experiment: plant=Squash; plant sample=QP3; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS02345 and LU632_RS02350 are separated by 325 nucleotides LU632_RS02350 and iolB are separated by 18 nucleotides iolB and LU632_RS02360 are separated by 83 nucleotides
LU632_RS02345: LU632_RS02345 - TIM barrel protein, at 460,689 to 461,486
_RS02345
LU632_RS02350: LU632_RS02350 - CoA-acylating methylmalonate-semialdehyde dehydrogenase, at 461,812 to 463,317
_RS02350
LU632_RS02355: iolB - 5-deoxy-glucuronate isomerase, at 463,336 to 464,145
iolB
LU632_RS02360: LU632_RS02360 - MurR/RpiR family transcriptional regulator, at 464,229 to 465,083
_RS02360
Position (kb) 461
462
463
464 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 460.865 kb on + strand, within LU632_RS02345 at 460.866 kb on - strand, within LU632_RS02345 at 460.866 kb on - strand, within LU632_RS02345 at 460.866 kb on - strand, within LU632_RS02345 at 460.947 kb on - strand, within LU632_RS02345 at 460.950 kb on + strand, within LU632_RS02345 at 460.950 kb on + strand, within LU632_RS02345 at 460.951 kb on - strand, within LU632_RS02345 at 460.982 kb on - strand, within LU632_RS02345 at 461.016 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.041 kb on - strand, within LU632_RS02345 at 461.109 kb on + strand, within LU632_RS02345 at 461.288 kb on + strand, within LU632_RS02345 at 461.289 kb on - strand, within LU632_RS02345 at 461.364 kb on - strand, within LU632_RS02345 at 461.384 kb on + strand, within LU632_RS02345 at 461.397 kb on + strand, within LU632_RS02345 at 461.397 kb on + strand, within LU632_RS02345 at 461.398 kb on - strand, within LU632_RS02345 at 461.415 kb on - strand at 461.818 kb on - strand at 462.014 kb on + strand, within LU632_RS02350 at 462.015 kb on - strand, within LU632_RS02350 at 462.015 kb on - strand, within LU632_RS02350 at 462.015 kb on - strand, within LU632_RS02350 at 462.070 kb on - strand, within LU632_RS02350 at 462.070 kb on - strand, within LU632_RS02350 at 462.088 kb on - strand, within LU632_RS02350 at 462.211 kb on + strand, within LU632_RS02350 at 462.212 kb on - strand, within LU632_RS02350 at 462.250 kb on - strand, within LU632_RS02350 at 462.250 kb on - strand, within LU632_RS02350 at 462.424 kb on - strand, within LU632_RS02350 at 462.448 kb on + strand, within LU632_RS02350 at 462.448 kb on + strand, within LU632_RS02350 at 462.482 kb on + strand, within LU632_RS02350 at 462.513 kb on + strand, within LU632_RS02350 at 462.690 kb on + strand, within LU632_RS02350 at 462.738 kb on - strand, within LU632_RS02350 at 462.866 kb on + strand, within LU632_RS02350 at 462.867 kb on - strand, within LU632_RS02350 at 462.877 kb on - strand, within LU632_RS02350 at 462.908 kb on - strand, within LU632_RS02350 at 462.910 kb on + strand, within LU632_RS02350 at 462.911 kb on - strand, within LU632_RS02350 at 462.911 kb on - strand, within LU632_RS02350 at 462.911 kb on - strand, within LU632_RS02350 at 463.009 kb on + strand, within LU632_RS02350 at 463.010 kb on - strand, within LU632_RS02350 at 463.061 kb on + strand, within LU632_RS02350 at 463.062 kb on - strand, within LU632_RS02350 at 463.074 kb on + strand, within LU632_RS02350 at 463.100 kb on + strand, within LU632_RS02350 at 463.100 kb on + strand, within LU632_RS02350 at 463.101 kb on - strand, within LU632_RS02350 at 463.372 kb on - strand at 463.616 kb on + strand, within iolB at 463.617 kb on - strand, within iolB at 463.667 kb on + strand, within iolB at 463.668 kb on - strand, within iolB at 463.704 kb on - strand, within iolB at 463.704 kb on - strand, within iolB at 463.709 kb on + strand, within iolB at 463.710 kb on - strand, within iolB at 463.732 kb on - strand, within iolB at 463.746 kb on - strand, within iolB at 463.753 kb on - strand, within iolB at 463.753 kb on - strand, within iolB at 463.839 kb on + strand, within iolB at 463.888 kb on + strand, within iolB at 463.888 kb on + strand, within iolB at 463.888 kb on + strand, within iolB at 463.889 kb on - strand, within iolB at 463.889 kb on - strand, within iolB at 463.892 kb on - strand, within iolB at 463.892 kb on - strand, within iolB at 463.937 kb on - strand, within iolB at 463.992 kb on + strand, within iolB at 464.044 kb on + strand, within iolB at 464.045 kb on - strand, within iolB at 464.045 kb on - strand, within iolB at 464.045 kb on - strand, within iolB at 464.045 kb on - strand, within iolB at 464.047 kb on + strand, within iolB at 464.047 kb on + strand, within iolB at 464.048 kb on - strand, within iolB at 464.048 kb on - strand, within iolB at 464.048 kb on - strand, within iolB at 464.076 kb on + strand at 464.077 kb on - strand at 464.115 kb on - strand at 464.125 kb on - strand at 464.125 kb on - strand at 464.129 kb on - strand at 464.129 kb on - strand at 464.184 kb on + strand at 464.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP3; plant location=NA remove 460,865 + LU632_RS02345 0.22 +1.2 460,866 - LU632_RS02345 0.22 -1.7 460,866 - LU632_RS02345 0.22 +1.6 460,866 - LU632_RS02345 0.22 -0.3 460,947 - LU632_RS02345 0.32 +0.6 460,950 + LU632_RS02345 0.33 +0.3 460,950 + LU632_RS02345 0.33 +0.4 460,951 - LU632_RS02345 0.33 -1.3 460,982 - LU632_RS02345 0.37 -1.6 461,016 - LU632_RS02345 0.41 +1.4 461,041 - LU632_RS02345 0.44 -2.5 461,041 - LU632_RS02345 0.44 -0.4 461,041 - LU632_RS02345 0.44 +0.3 461,041 - LU632_RS02345 0.44 -1.2 461,041 - LU632_RS02345 0.44 +0.0 461,109 + LU632_RS02345 0.53 -0.0 461,288 + LU632_RS02345 0.75 +0.4 461,289 - LU632_RS02345 0.75 +0.6 461,364 - LU632_RS02345 0.85 -1.4 461,384 + LU632_RS02345 0.87 +1.3 461,397 + LU632_RS02345 0.89 -0.5 461,397 + LU632_RS02345 0.89 +0.4 461,398 - LU632_RS02345 0.89 -0.7 461,415 - -2.1 461,818 - +0.7 462,014 + LU632_RS02350 0.13 +2.0 462,015 - LU632_RS02350 0.13 -0.4 462,015 - LU632_RS02350 0.13 -1.6 462,015 - LU632_RS02350 0.13 -0.6 462,070 - LU632_RS02350 0.17 -1.0 462,070 - LU632_RS02350 0.17 -0.4 462,088 - LU632_RS02350 0.18 +0.0 462,211 + LU632_RS02350 0.26 +0.6 462,212 - LU632_RS02350 0.27 -2.7 462,250 - LU632_RS02350 0.29 -0.5 462,250 - LU632_RS02350 0.29 -0.0 462,424 - LU632_RS02350 0.41 +0.6 462,448 + LU632_RS02350 0.42 -0.1 462,448 + LU632_RS02350 0.42 +2.1 462,482 + LU632_RS02350 0.44 -0.6 462,513 + LU632_RS02350 0.47 +1.5 462,690 + LU632_RS02350 0.58 +0.6 462,738 - LU632_RS02350 0.61 -2.3 462,866 + LU632_RS02350 0.70 -0.6 462,867 - LU632_RS02350 0.70 -0.7 462,877 - LU632_RS02350 0.71 +0.0 462,908 - LU632_RS02350 0.73 +0.4 462,910 + LU632_RS02350 0.73 -0.1 462,911 - LU632_RS02350 0.73 -0.6 462,911 - LU632_RS02350 0.73 -0.9 462,911 - LU632_RS02350 0.73 -0.6 463,009 + LU632_RS02350 0.79 +0.9 463,010 - LU632_RS02350 0.80 -0.8 463,061 + LU632_RS02350 0.83 -0.4 463,062 - LU632_RS02350 0.83 -0.7 463,074 + LU632_RS02350 0.84 -1.4 463,100 + LU632_RS02350 0.86 -0.3 463,100 + LU632_RS02350 0.86 +0.6 463,101 - LU632_RS02350 0.86 +0.2 463,372 - -1.4 463,616 + iolB LU632_RS02355 0.35 -0.1 463,617 - iolB LU632_RS02355 0.35 -0.1 463,667 + iolB LU632_RS02355 0.41 +0.5 463,668 - iolB LU632_RS02355 0.41 -0.1 463,704 - iolB LU632_RS02355 0.45 -1.2 463,704 - iolB LU632_RS02355 0.45 +0.6 463,709 + iolB LU632_RS02355 0.46 -0.4 463,710 - iolB LU632_RS02355 0.46 +4.2 463,732 - iolB LU632_RS02355 0.49 +0.1 463,746 - iolB LU632_RS02355 0.51 +0.8 463,753 - iolB LU632_RS02355 0.51 +0.0 463,753 - iolB LU632_RS02355 0.51 +1.0 463,839 + iolB LU632_RS02355 0.62 -0.5 463,888 + iolB LU632_RS02355 0.68 -1.5 463,888 + iolB LU632_RS02355 0.68 +2.4 463,888 + iolB LU632_RS02355 0.68 +1.1 463,889 - iolB LU632_RS02355 0.68 +1.9 463,889 - iolB LU632_RS02355 0.68 +0.7 463,892 - iolB LU632_RS02355 0.69 -1.0 463,892 - iolB LU632_RS02355 0.69 +3.4 463,937 - iolB LU632_RS02355 0.74 -2.1 463,992 + iolB LU632_RS02355 0.81 +0.7 464,044 + iolB LU632_RS02355 0.87 -0.4 464,045 - iolB LU632_RS02355 0.88 +1.0 464,045 - iolB LU632_RS02355 0.88 -1.1 464,045 - iolB LU632_RS02355 0.88 -1.1 464,045 - iolB LU632_RS02355 0.88 -0.4 464,047 + iolB LU632_RS02355 0.88 +0.0 464,047 + iolB LU632_RS02355 0.88 -0.1 464,048 - iolB LU632_RS02355 0.88 -0.5 464,048 - iolB LU632_RS02355 0.88 -0.1 464,048 - iolB LU632_RS02355 0.88 +0.2 464,076 + -2.6 464,077 - +0.0 464,115 - +0.5 464,125 - +0.0 464,125 - -2.8 464,129 - +0.6 464,129 - +0.7 464,184 + +0.0 464,230 + +3.0
Or see this region's nucleotide sequence