Experiment: plant=Squash; plant sample=QP3; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS02075 and LU632_RS02080 are separated by 146 nucleotides LU632_RS02080 and cysQ are separated by 312 nucleotides cysQ and LU632_RS02090 are separated by 41 nucleotides LU632_RS02090 and LU632_RS02095 are separated by 303 nucleotides
LU632_RS02075: LU632_RS02075 - transposase, at 408,670 to 409,005
_RS02075
LU632_RS02080: LU632_RS02080 - hypothetical protein, at 409,152 to 409,565
_RS02080
LU632_RS02085: cysQ - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 409,878 to 410,618
cysQ
LU632_RS02090: LU632_RS02090 - YtfJ family protein, at 410,660 to 411,242
_RS02090
LU632_RS02095: LU632_RS02095 - DUF1107 domain-containing protein, at 411,546 to 411,752
_RS02095
Position (kb) 409
410
411 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 408.878 kb on + strand, within LU632_RS02075 at 408.878 kb on + strand, within LU632_RS02075 at 408.956 kb on - strand, within LU632_RS02075 at 409.065 kb on - strand at 409.068 kb on + strand at 409.069 kb on - strand at 409.069 kb on - strand at 409.069 kb on - strand at 409.098 kb on - strand at 409.106 kb on + strand at 409.130 kb on + strand at 409.138 kb on - strand at 409.165 kb on - strand at 409.221 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.223 kb on - strand, within LU632_RS02080 at 409.272 kb on - strand, within LU632_RS02080 at 409.315 kb on - strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.335 kb on + strand, within LU632_RS02080 at 409.336 kb on - strand, within LU632_RS02080 at 409.336 kb on - strand, within LU632_RS02080 at 409.498 kb on + strand, within LU632_RS02080 at 409.498 kb on + strand, within LU632_RS02080 at 409.499 kb on - strand, within LU632_RS02080 at 409.560 kb on + strand at 409.561 kb on - strand at 409.561 kb on - strand at 409.607 kb on - strand at 409.639 kb on - strand at 409.704 kb on - strand at 409.833 kb on + strand at 409.833 kb on + strand at 409.834 kb on - strand at 409.834 kb on - strand at 409.838 kb on - strand at 409.838 kb on - strand at 409.860 kb on + strand at 409.860 kb on + strand at 409.861 kb on - strand at 409.861 kb on - strand at 409.933 kb on + strand at 409.933 kb on + strand at 409.934 kb on - strand at 409.935 kb on + strand at 409.935 kb on + strand at 409.936 kb on - strand at 410.244 kb on - strand, within cysQ at 410.477 kb on + strand, within cysQ at 410.477 kb on + strand, within cysQ at 410.566 kb on - strand at 410.566 kb on - strand at 410.566 kb on - strand at 410.791 kb on + strand, within LU632_RS02090 at 410.855 kb on - strand, within LU632_RS02090 at 410.897 kb on - strand, within LU632_RS02090 at 411.002 kb on + strand, within LU632_RS02090 at 411.043 kb on + strand, within LU632_RS02090 at 411.124 kb on - strand, within LU632_RS02090 at 411.168 kb on + strand, within LU632_RS02090 at 411.171 kb on - strand, within LU632_RS02090 at 411.263 kb on + strand at 411.330 kb on - strand at 411.330 kb on - strand at 411.339 kb on + strand at 411.370 kb on + strand at 411.382 kb on + strand at 411.383 kb on - strand at 411.468 kb on + strand at 411.468 kb on + strand at 411.584 kb on + strand, within LU632_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP3; plant location=NA remove 408,878 + LU632_RS02075 0.62 +0.6 408,878 + LU632_RS02075 0.62 -0.5 408,956 - LU632_RS02075 0.85 +1.1 409,065 - -0.1 409,068 + -0.2 409,069 - +2.2 409,069 - +0.1 409,069 - +0.2 409,098 - -0.6 409,106 + -1.2 409,130 + +0.3 409,138 - +0.9 409,165 - +2.0 409,221 - LU632_RS02080 0.17 +4.2 409,223 - LU632_RS02080 0.17 -0.2 409,223 - LU632_RS02080 0.17 +0.5 409,223 - LU632_RS02080 0.17 -0.7 409,272 - LU632_RS02080 0.29 +0.2 409,315 - LU632_RS02080 0.39 +0.1 409,335 + LU632_RS02080 0.44 +0.4 409,335 + LU632_RS02080 0.44 -0.5 409,335 + LU632_RS02080 0.44 -0.9 409,336 - LU632_RS02080 0.44 -0.1 409,336 - LU632_RS02080 0.44 +0.9 409,498 + LU632_RS02080 0.84 -2.2 409,498 + LU632_RS02080 0.84 +0.7 409,499 - LU632_RS02080 0.84 -3.4 409,560 + +0.3 409,561 - +2.0 409,561 - -1.2 409,607 - +1.6 409,639 - +1.1 409,704 - +0.3 409,833 + -1.2 409,833 + -0.0 409,834 - -1.4 409,834 - -0.2 409,838 - -1.7 409,838 - +1.3 409,860 + +1.3 409,860 + -0.9 409,861 - -0.8 409,861 - -1.9 409,933 + -0.0 409,933 + -1.0 409,934 - -0.9 409,935 + -2.2 409,935 + +0.6 409,936 - +0.3 410,244 - cysQ LU632_RS02085 0.49 +0.9 410,477 + cysQ LU632_RS02085 0.81 +0.6 410,477 + cysQ LU632_RS02085 0.81 +0.6 410,566 - +3.2 410,566 - -1.7 410,566 - -0.1 410,791 + LU632_RS02090 0.22 +0.4 410,855 - LU632_RS02090 0.33 -0.5 410,897 - LU632_RS02090 0.41 -1.4 411,002 + LU632_RS02090 0.59 +1.4 411,043 + LU632_RS02090 0.66 +0.1 411,124 - LU632_RS02090 0.80 +0.7 411,168 + LU632_RS02090 0.87 -0.9 411,171 - LU632_RS02090 0.88 +0.6 411,263 + -0.7 411,330 - +0.9 411,330 - -1.3 411,339 + +0.6 411,370 + -0.2 411,382 + -0.9 411,383 - -0.4 411,468 + +1.1 411,468 + -0.7 411,584 + LU632_RS02095 0.18 +0.1
Or see this region's nucleotide sequence