Experiment: plant=Squash; plant sample=QP2; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gluQRS and pcnB are separated by 106 nucleotides pcnB and folK overlap by 4 nucleotides folK and panB are separated by 60 nucleotides
LU632_RS20085: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 3,826,027 to 3,826,932
gluQRS
LU632_RS20090: pcnB - polynucleotide adenylyltransferase PcnB, at 3,827,039 to 3,828,454
pcnB
LU632_RS20095: folK - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,828,451 to 3,828,930
folK
LU632_RS20100: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,828,991 to 3,829,785
panB
Position (kb)
3827
3828
3829 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 3826.039 kb on + strand at 3826.039 kb on + strand at 3826.039 kb on + strand at 3826.039 kb on + strand at 3826.040 kb on - strand at 3826.040 kb on - strand at 3826.041 kb on + strand at 3826.111 kb on + strand at 3826.112 kb on - strand at 3826.112 kb on - strand at 3826.112 kb on - strand at 3826.318 kb on + strand, within gluQRS at 3826.318 kb on + strand, within gluQRS at 3826.388 kb on + strand, within gluQRS at 3826.389 kb on - strand, within gluQRS at 3826.389 kb on - strand, within gluQRS at 3826.537 kb on + strand, within gluQRS at 3826.537 kb on + strand, within gluQRS at 3826.538 kb on - strand, within gluQRS at 3826.540 kb on - strand, within gluQRS at 3826.586 kb on + strand, within gluQRS at 3826.636 kb on + strand, within gluQRS at 3826.637 kb on - strand, within gluQRS at 3826.666 kb on + strand, within gluQRS at 3826.677 kb on - strand, within gluQRS at 3826.817 kb on + strand, within gluQRS at 3826.817 kb on + strand, within gluQRS at 3826.850 kb on + strand at 3826.850 kb on + strand at 3826.899 kb on + strand at 3826.899 kb on + strand at 3826.983 kb on + strand at 3826.984 kb on - strand at 3827.549 kb on + strand, within pcnB at 3827.552 kb on + strand, within pcnB at 3827.552 kb on + strand, within pcnB at 3827.893 kb on + strand, within pcnB at 3828.216 kb on + strand, within pcnB at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.959 kb on - strand at 3828.974 kb on + strand at 3829.063 kb on + strand at 3829.072 kb on - strand, within panB at 3829.189 kb on + strand, within panB at 3829.189 kb on + strand, within panB at 3829.259 kb on - strand, within panB at 3829.300 kb on - strand, within panB at 3829.317 kb on + strand, within panB at 3829.318 kb on - strand, within panB
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP2; plant location=NA remove 3,826,039 + +1.3 3,826,039 + +0.1 3,826,039 + -0.7 3,826,039 + -1.7 3,826,040 - +1.6 3,826,040 - +0.8 3,826,041 + -1.2 3,826,111 + +1.2 3,826,112 - +0.8 3,826,112 - -0.3 3,826,112 - -2.5 3,826,318 + gluQRS LU632_RS20085 0.32 -0.5 3,826,318 + gluQRS LU632_RS20085 0.32 +0.7 3,826,388 + gluQRS LU632_RS20085 0.40 -0.7 3,826,389 - gluQRS LU632_RS20085 0.40 +1.4 3,826,389 - gluQRS LU632_RS20085 0.40 +1.4 3,826,537 + gluQRS LU632_RS20085 0.56 +0.7 3,826,537 + gluQRS LU632_RS20085 0.56 -0.3 3,826,538 - gluQRS LU632_RS20085 0.56 -1.1 3,826,540 - gluQRS LU632_RS20085 0.57 +2.2 3,826,586 + gluQRS LU632_RS20085 0.62 -0.4 3,826,636 + gluQRS LU632_RS20085 0.67 +0.0 3,826,637 - gluQRS LU632_RS20085 0.67 +0.2 3,826,666 + gluQRS LU632_RS20085 0.71 -0.4 3,826,677 - gluQRS LU632_RS20085 0.72 +3.1 3,826,817 + gluQRS LU632_RS20085 0.87 -0.3 3,826,817 + gluQRS LU632_RS20085 0.87 -0.6 3,826,850 + +1.0 3,826,850 + -0.6 3,826,899 + -0.2 3,826,899 + -0.5 3,826,983 + -0.8 3,826,984 - -0.1 3,827,549 + pcnB LU632_RS20090 0.36 +2.4 3,827,552 + pcnB LU632_RS20090 0.36 -0.7 3,827,552 + pcnB LU632_RS20090 0.36 +0.8 3,827,893 + pcnB LU632_RS20090 0.60 +1.5 3,828,216 + pcnB LU632_RS20090 0.83 +6.0 3,828,958 + +1.3 3,828,958 + +1.3 3,828,958 + -0.8 3,828,959 - +0.7 3,828,974 + +0.8 3,829,063 + -1.3 3,829,072 - panB LU632_RS20100 0.10 +0.1 3,829,189 + panB LU632_RS20100 0.25 -1.5 3,829,189 + panB LU632_RS20100 0.25 +1.3 3,829,259 - panB LU632_RS20100 0.34 -0.8 3,829,300 - panB LU632_RS20100 0.39 +2.7 3,829,317 + panB LU632_RS20100 0.41 +2.1 3,829,318 - panB LU632_RS20100 0.41 +1.9
Or see this region's nucleotide sequence