Experiment: plant=Squash; plant sample=QP2; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pabB and LU632_RS13180 overlap by 8 nucleotides LU632_RS13180 and sdaA are separated by 365 nucleotides
LU632_RS13175: pabB - aminodeoxychorismate synthase component 1, at 2,513,567 to 2,514,931
pabB
LU632_RS13180: LU632_RS13180 - CoA pyrophosphatase, at 2,514,924 to 2,515,497
_RS13180
LU632_RS13185: sdaA - L-serine ammonia-lyase, at 2,515,863 to 2,517,227
sdaA
Position (kb)
2514
2515
2516 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2513.971 kb on - strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.973 kb on - strand, within pabB at 2514.050 kb on - strand, within pabB at 2514.137 kb on + strand, within pabB at 2514.137 kb on + strand, within pabB at 2514.138 kb on - strand, within pabB at 2514.604 kb on + strand, within pabB at 2514.746 kb on + strand, within pabB at 2514.779 kb on - strand, within pabB at 2514.781 kb on + strand, within pabB at 2514.781 kb on + strand, within pabB at 2514.782 kb on - strand, within pabB at 2514.785 kb on - strand, within pabB at 2514.957 kb on - strand at 2515.040 kb on + strand, within LU632_RS13180 at 2515.212 kb on + strand, within LU632_RS13180 at 2515.212 kb on + strand, within LU632_RS13180 at 2515.212 kb on + strand, within LU632_RS13180 at 2515.589 kb on + strand at 2515.592 kb on + strand at 2515.592 kb on + strand at 2515.593 kb on - strand at 2515.593 kb on - strand at 2515.611 kb on + strand at 2515.612 kb on - strand at 2515.618 kb on + strand at 2515.619 kb on - strand at 2515.624 kb on - strand at 2515.636 kb on - strand at 2515.671 kb on + strand at 2515.679 kb on - strand at 2515.679 kb on - strand at 2515.734 kb on - strand at 2515.811 kb on - strand at 2515.811 kb on - strand at 2515.813 kb on + strand at 2515.814 kb on - strand at 2515.816 kb on + strand at 2515.840 kb on - strand at 2515.869 kb on - strand at 2515.916 kb on + strand at 2515.967 kb on + strand at 2516.033 kb on + strand, within sdaA at 2516.072 kb on - strand, within sdaA at 2516.072 kb on - strand, within sdaA at 2516.289 kb on + strand, within sdaA at 2516.497 kb on + strand, within sdaA
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP2; plant location=NA remove 2,513,971 - pabB LU632_RS13175 0.30 +1.0 2,513,972 + pabB LU632_RS13175 0.30 +2.8 2,513,972 + pabB LU632_RS13175 0.30 -0.8 2,513,972 + pabB LU632_RS13175 0.30 +1.5 2,513,972 + pabB LU632_RS13175 0.30 -2.5 2,513,972 + pabB LU632_RS13175 0.30 +4.5 2,513,973 - pabB LU632_RS13175 0.30 +0.4 2,514,050 - pabB LU632_RS13175 0.35 +1.3 2,514,137 + pabB LU632_RS13175 0.42 +0.4 2,514,137 + pabB LU632_RS13175 0.42 -1.1 2,514,138 - pabB LU632_RS13175 0.42 -0.5 2,514,604 + pabB LU632_RS13175 0.76 -0.8 2,514,746 + pabB LU632_RS13175 0.86 +0.6 2,514,779 - pabB LU632_RS13175 0.89 -0.3 2,514,781 + pabB LU632_RS13175 0.89 +0.0 2,514,781 + pabB LU632_RS13175 0.89 +0.2 2,514,782 - pabB LU632_RS13175 0.89 -0.7 2,514,785 - pabB LU632_RS13175 0.89 -0.0 2,514,957 - -0.4 2,515,040 + LU632_RS13180 0.20 +4.8 2,515,212 + LU632_RS13180 0.50 -0.7 2,515,212 + LU632_RS13180 0.50 +1.3 2,515,212 + LU632_RS13180 0.50 -1.0 2,515,589 + +0.1 2,515,592 + +0.8 2,515,592 + -0.7 2,515,593 - -0.3 2,515,593 - +0.8 2,515,611 + -2.3 2,515,612 - -0.3 2,515,618 + +0.0 2,515,619 - -0.5 2,515,624 - -1.4 2,515,636 - -1.7 2,515,671 + +0.5 2,515,679 - +0.3 2,515,679 - -1.4 2,515,734 - +0.9 2,515,811 - -1.7 2,515,811 - -1.5 2,515,813 + +1.3 2,515,814 - +1.3 2,515,816 + -0.5 2,515,840 - -0.3 2,515,869 - -0.8 2,515,916 + +0.4 2,515,967 + +0.9 2,516,033 + sdaA LU632_RS13185 0.12 +2.6 2,516,072 - sdaA LU632_RS13185 0.15 +0.6 2,516,072 - sdaA LU632_RS13185 0.15 -0.6 2,516,289 + sdaA LU632_RS13185 0.31 -2.0 2,516,497 + sdaA LU632_RS13185 0.46 +0.7
Or see this region's nucleotide sequence