Experiment: plant=Squash; plant sample=QP2; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides hisA and hisH are separated by 5 nucleotides hisH and hisB are separated by 0 nucleotides
LU632_RS08915: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 1,683,482 to 1,684,093
hisIE
LU632_RS08920: hisF - imidazole glycerol phosphate synthase subunit HisF, at 1,684,087 to 1,684,863
hisF
LU632_RS08925: hisA - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,684,845 to 1,685,582
hisA
LU632_RS08930: hisH - imidazole glycerol phosphate synthase subunit HisH, at 1,685,588 to 1,686,178
hisH
LU632_RS08935: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,686,179 to 1,687,246
hisB
Position (kb)
1684
1685
1686 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1683.985 kb on + strand, within hisIE at 1683.985 kb on + strand, within hisIE at 1683.986 kb on - strand, within hisIE at 1683.987 kb on + strand, within hisIE at 1683.987 kb on + strand, within hisIE at 1683.988 kb on - strand, within hisIE at 1684.042 kb on + strand at 1684.043 kb on - strand at 1684.146 kb on + strand at 1684.146 kb on + strand at 1684.146 kb on + strand at 1684.149 kb on + strand at 1684.263 kb on + strand, within hisF at 1684.263 kb on + strand, within hisF at 1684.263 kb on + strand, within hisF at 1684.264 kb on - strand, within hisF at 1684.332 kb on + strand, within hisF at 1684.332 kb on + strand, within hisF at 1684.336 kb on - strand, within hisF at 1684.431 kb on - strand, within hisF at 1684.445 kb on + strand, within hisF at 1684.466 kb on + strand, within hisF at 1684.466 kb on + strand, within hisF at 1684.467 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.801 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand at 1685.234 kb on + strand, within hisA at 1685.234 kb on + strand, within hisA at 1685.471 kb on - strand, within hisA at 1685.471 kb on - strand, within hisA at 1685.478 kb on + strand, within hisA at 1685.503 kb on + strand, within hisA at 1685.576 kb on + strand at 1685.589 kb on + strand at 1685.590 kb on - strand at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.703 kb on + strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.704 kb on - strand, within hisH at 1685.707 kb on + strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.708 kb on - strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.745 kb on + strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.746 kb on - strand, within hisH at 1685.760 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.766 kb on + strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH at 1685.767 kb on - strand, within hisH at 1686.181 kb on - strand at 1686.181 kb on - strand at 1686.368 kb on + strand, within hisB at 1686.391 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.404 kb on - strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.573 kb on + strand, within hisB at 1686.574 kb on - strand, within hisB at 1686.574 kb on - strand, within hisB
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP2; plant location=NA remove 1,683,985 + hisIE LU632_RS08915 0.82 +1.1 1,683,985 + hisIE LU632_RS08915 0.82 -1.8 1,683,986 - hisIE LU632_RS08915 0.82 -0.6 1,683,987 + hisIE LU632_RS08915 0.83 -0.6 1,683,987 + hisIE LU632_RS08915 0.83 -1.6 1,683,988 - hisIE LU632_RS08915 0.83 -3.0 1,684,042 + -1.2 1,684,043 - -3.5 1,684,146 + +2.4 1,684,146 + -0.8 1,684,146 + -1.1 1,684,149 + -0.9 1,684,263 + hisF LU632_RS08920 0.23 +0.6 1,684,263 + hisF LU632_RS08920 0.23 -0.6 1,684,263 + hisF LU632_RS08920 0.23 +0.4 1,684,264 - hisF LU632_RS08920 0.23 -2.2 1,684,332 + hisF LU632_RS08920 0.32 -1.0 1,684,332 + hisF LU632_RS08920 0.32 -3.1 1,684,336 - hisF LU632_RS08920 0.32 -2.1 1,684,431 - hisF LU632_RS08920 0.44 -0.7 1,684,445 + hisF LU632_RS08920 0.46 -2.9 1,684,466 + hisF LU632_RS08920 0.49 -1.2 1,684,466 + hisF LU632_RS08920 0.49 -1.8 1,684,467 - hisF LU632_RS08920 0.49 +0.1 1,684,589 - hisF LU632_RS08920 0.65 -1.4 1,684,589 - hisF LU632_RS08920 0.65 -0.9 1,684,589 - hisF LU632_RS08920 0.65 -0.2 1,684,801 + -1.8 1,684,866 + -1.2 1,684,866 + +0.8 1,684,866 + -0.2 1,685,234 + hisA LU632_RS08925 0.53 -0.9 1,685,234 + hisA LU632_RS08925 0.53 -1.2 1,685,471 - hisA LU632_RS08925 0.85 +0.4 1,685,471 - hisA LU632_RS08925 0.85 -1.7 1,685,478 + hisA LU632_RS08925 0.86 -0.9 1,685,503 + hisA LU632_RS08925 0.89 -1.9 1,685,576 + -2.2 1,685,589 + -1.8 1,685,590 - -0.2 1,685,703 + hisH LU632_RS08930 0.19 -1.2 1,685,703 + hisH LU632_RS08930 0.19 -2.3 1,685,703 + hisH LU632_RS08930 0.19 -0.6 1,685,704 - hisH LU632_RS08930 0.20 -0.9 1,685,704 - hisH LU632_RS08930 0.20 -1.5 1,685,707 + hisH LU632_RS08930 0.20 -0.9 1,685,708 - hisH LU632_RS08930 0.20 -1.7 1,685,708 - hisH LU632_RS08930 0.20 -0.5 1,685,708 - hisH LU632_RS08930 0.20 -1.1 1,685,708 - hisH LU632_RS08930 0.20 +1.9 1,685,708 - hisH LU632_RS08930 0.20 -1.0 1,685,745 + hisH LU632_RS08930 0.27 -1.8 1,685,745 + hisH LU632_RS08930 0.27 -2.8 1,685,746 - hisH LU632_RS08930 0.27 +0.4 1,685,746 - hisH LU632_RS08930 0.27 -2.3 1,685,746 - hisH LU632_RS08930 0.27 +0.4 1,685,760 + hisH LU632_RS08930 0.29 -2.4 1,685,766 + hisH LU632_RS08930 0.30 +1.4 1,685,766 + hisH LU632_RS08930 0.30 -1.9 1,685,767 - hisH LU632_RS08930 0.30 +0.2 1,685,767 - hisH LU632_RS08930 0.30 -2.2 1,685,767 - hisH LU632_RS08930 0.30 -2.1 1,686,181 - +1.0 1,686,181 - -1.3 1,686,368 + hisB LU632_RS08935 0.18 -0.4 1,686,391 + hisB LU632_RS08935 0.20 -0.6 1,686,403 + hisB LU632_RS08935 0.21 -2.3 1,686,403 + hisB LU632_RS08935 0.21 -2.1 1,686,403 + hisB LU632_RS08935 0.21 -1.3 1,686,404 - hisB LU632_RS08935 0.21 +1.4 1,686,405 + hisB LU632_RS08935 0.21 -1.6 1,686,405 + hisB LU632_RS08935 0.21 -1.1 1,686,573 + hisB LU632_RS08935 0.37 +0.4 1,686,574 - hisB LU632_RS08935 0.37 -0.2 1,686,574 - hisB LU632_RS08935 0.37 +1.4
Or see this region's nucleotide sequence