Experiment: plant=Squash; plant sample=QP2; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truD and surE are separated by 532 nucleotides surE and LU632_RS06470 overlap by 7 nucleotides LU632_RS06470 and nlpD are separated by 143 nucleotides
LU632_RS06460: truD - tRNA pseudouridine(13) synthase TruD, at 1,241,251 to 1,242,300
truD
LU632_RS06465: surE - 5'/3'-nucleotidase SurE, at 1,242,833 to 1,243,594
surE
LU632_RS06470: LU632_RS06470 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 1,243,588 to 1,244,214
_RS06470
LU632_RS06475: nlpD - murein hydrolase activator NlpD, at 1,244,358 to 1,245,473
nlpD
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1241.852 kb on + strand, within truD at 1241.857 kb on - strand, within truD at 1241.998 kb on - strand, within truD at 1241.998 kb on - strand, within truD at 1241.998 kb on - strand, within truD at 1242.159 kb on - strand, within truD at 1242.159 kb on - strand, within truD at 1242.249 kb on - strand at 1242.263 kb on + strand at 1242.360 kb on + strand at 1242.418 kb on + strand at 1242.418 kb on + strand at 1242.418 kb on + strand at 1242.419 kb on - strand at 1242.419 kb on - strand at 1242.459 kb on - strand at 1242.546 kb on + strand at 1242.585 kb on + strand at 1242.586 kb on - strand at 1242.644 kb on + strand at 1242.705 kb on + strand at 1242.716 kb on - strand at 1242.779 kb on + strand at 1242.779 kb on + strand at 1242.779 kb on + strand at 1242.780 kb on - strand at 1242.862 kb on + strand at 1243.063 kb on + strand, within surE at 1243.185 kb on + strand, within surE at 1243.414 kb on + strand, within surE at 1243.592 kb on + strand at 1243.771 kb on + strand, within LU632_RS06470 at 1243.771 kb on + strand, within LU632_RS06470 at 1243.771 kb on + strand, within LU632_RS06470 at 1243.772 kb on - strand, within LU632_RS06470 at 1243.773 kb on + strand, within LU632_RS06470 at 1243.773 kb on + strand, within LU632_RS06470 at 1243.773 kb on + strand, within LU632_RS06470 at 1244.011 kb on + strand, within LU632_RS06470 at 1244.062 kb on - strand, within LU632_RS06470 at 1244.231 kb on + strand at 1244.231 kb on + strand at 1244.279 kb on + strand at 1244.335 kb on + strand at 1244.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP2; plant location=NA remove 1,241,852 + truD LU632_RS06460 0.57 -0.1 1,241,857 - truD LU632_RS06460 0.58 -0.1 1,241,998 - truD LU632_RS06460 0.71 -1.8 1,241,998 - truD LU632_RS06460 0.71 -0.8 1,241,998 - truD LU632_RS06460 0.71 +2.7 1,242,159 - truD LU632_RS06460 0.86 +0.4 1,242,159 - truD LU632_RS06460 0.86 +1.1 1,242,249 - -0.6 1,242,263 + +0.2 1,242,360 + -0.9 1,242,418 + +0.7 1,242,418 + -1.1 1,242,418 + +0.5 1,242,419 - -2.5 1,242,419 - -2.0 1,242,459 - -0.2 1,242,546 + +1.6 1,242,585 + +3.5 1,242,586 - -0.1 1,242,644 + -0.1 1,242,705 + -0.7 1,242,716 - +0.5 1,242,779 + +0.2 1,242,779 + -0.1 1,242,779 + +1.6 1,242,780 - -0.3 1,242,862 + -0.2 1,243,063 + surE LU632_RS06465 0.30 -0.1 1,243,185 + surE LU632_RS06465 0.46 +0.5 1,243,414 + surE LU632_RS06465 0.76 +0.2 1,243,592 + +1.3 1,243,771 + LU632_RS06470 0.29 -1.3 1,243,771 + LU632_RS06470 0.29 -1.3 1,243,771 + LU632_RS06470 0.29 -0.5 1,243,772 - LU632_RS06470 0.29 -0.3 1,243,773 + LU632_RS06470 0.30 -0.1 1,243,773 + LU632_RS06470 0.30 -1.1 1,243,773 + LU632_RS06470 0.30 -0.3 1,244,011 + LU632_RS06470 0.67 +1.5 1,244,062 - LU632_RS06470 0.76 +3.1 1,244,231 + -0.5 1,244,231 + +1.5 1,244,279 + +1.4 1,244,335 + +0.6 1,244,386 + -0.5
Or see this region's nucleotide sequence