Experiment: plant=Squash; plant sample=QP1; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroE and tsaC are separated by 5 nucleotides tsaC and LU632_RS22455 overlap by 8 nucleotides LU632_RS22455 and smg are separated by 27 nucleotides smg and dprA overlap by 29 nucleotides
LU632_RS22445: aroE - shikimate dehydrogenase, at 4,290,060 to 4,290,881
aroE
LU632_RS22450: tsaC - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 4,290,887 to 4,291,459
tsaC
LU632_RS22455: LU632_RS22455 - topoisomerase DNA-binding C4 zinc finger domain-containing protein, at 4,291,452 to 4,292,006
_RS22455
LU632_RS22460: smg - DUF494 family protein Smg, at 4,292,034 to 4,292,507
smg
LU632_RS22465: dprA - DNA-protecting protein DprA, at 4,292,479 to 4,293,603
dprA
Position (kb) 4291
4292
4293 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4290.475 kb on - strand, within aroE at 4290.620 kb on + strand, within aroE at 4290.686 kb on + strand, within aroE at 4290.728 kb on + strand, within aroE at 4290.822 kb on + strand at 4290.822 kb on + strand at 4290.823 kb on - strand at 4290.823 kb on - strand at 4290.823 kb on - strand at 4290.825 kb on - strand at 4291.755 kb on - strand, within LU632_RS22455 at 4291.755 kb on - strand, within LU632_RS22455 at 4291.820 kb on - strand, within LU632_RS22455 at 4291.880 kb on - strand, within LU632_RS22455 at 4292.006 kb on + strand at 4292.008 kb on + strand at 4292.008 kb on + strand at 4292.009 kb on - strand at 4292.009 kb on - strand at 4292.013 kb on + strand at 4292.013 kb on + strand at 4292.013 kb on + strand at 4292.014 kb on - strand at 4292.014 kb on - strand at 4292.140 kb on - strand, within smg at 4292.265 kb on + strand, within smg at 4292.265 kb on + strand, within smg at 4292.266 kb on - strand, within smg at 4292.266 kb on - strand, within smg at 4292.291 kb on - strand, within smg at 4292.294 kb on + strand, within smg at 4292.295 kb on - strand, within smg at 4292.387 kb on - strand, within smg at 4292.430 kb on + strand, within smg at 4292.435 kb on + strand, within smg at 4292.473 kb on + strand at 4292.474 kb on - strand at 4292.474 kb on - strand at 4292.488 kb on + strand at 4292.488 kb on + strand at 4292.488 kb on + strand at 4292.489 kb on - strand at 4292.489 kb on - strand at 4292.489 kb on - strand at 4292.489 kb on - strand at 4292.493 kb on + strand at 4292.494 kb on - strand at 4292.532 kb on - strand at 4292.688 kb on + strand, within dprA at 4292.689 kb on - strand, within dprA at 4292.708 kb on + strand, within dprA at 4292.708 kb on + strand, within dprA at 4292.709 kb on - strand, within dprA at 4292.709 kb on - strand, within dprA at 4292.710 kb on + strand, within dprA at 4292.772 kb on + strand, within dprA at 4292.773 kb on - strand, within dprA at 4292.821 kb on - strand, within dprA at 4292.930 kb on - strand, within dprA at 4292.930 kb on - strand, within dprA at 4292.930 kb on - strand, within dprA at 4292.962 kb on + strand, within dprA
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP1; plant location=NA remove 4,290,475 - aroE LU632_RS22445 0.50 -0.0 4,290,620 + aroE LU632_RS22445 0.68 +2.0 4,290,686 + aroE LU632_RS22445 0.76 -0.7 4,290,728 + aroE LU632_RS22445 0.81 +1.0 4,290,822 + -0.6 4,290,822 + +2.0 4,290,823 - +1.4 4,290,823 - -1.0 4,290,823 - +0.7 4,290,825 - +2.0 4,291,755 - LU632_RS22455 0.55 -0.0 4,291,755 - LU632_RS22455 0.55 +1.0 4,291,820 - LU632_RS22455 0.66 +0.7 4,291,880 - LU632_RS22455 0.77 -2.3 4,292,006 + -1.0 4,292,008 + +0.9 4,292,008 + -1.4 4,292,009 - +0.5 4,292,009 - -1.3 4,292,013 + -1.5 4,292,013 + +1.4 4,292,013 + +0.7 4,292,014 - +0.3 4,292,014 - -0.4 4,292,140 - smg LU632_RS22460 0.22 -1.1 4,292,265 + smg LU632_RS22460 0.49 +2.0 4,292,265 + smg LU632_RS22460 0.49 +1.0 4,292,266 - smg LU632_RS22460 0.49 -0.8 4,292,266 - smg LU632_RS22460 0.49 -0.6 4,292,291 - smg LU632_RS22460 0.54 -0.2 4,292,294 + smg LU632_RS22460 0.55 -0.4 4,292,295 - smg LU632_RS22460 0.55 +0.8 4,292,387 - smg LU632_RS22460 0.74 -2.1 4,292,430 + smg LU632_RS22460 0.84 -1.6 4,292,435 + smg LU632_RS22460 0.85 +0.8 4,292,473 + -0.6 4,292,474 - +0.2 4,292,474 - -2.4 4,292,488 + -1.8 4,292,488 + -0.3 4,292,488 + -0.9 4,292,489 - -0.2 4,292,489 - -0.3 4,292,489 - +0.1 4,292,489 - +2.0 4,292,493 + -1.1 4,292,494 - -0.6 4,292,532 - +1.4 4,292,688 + dprA LU632_RS22465 0.19 +0.9 4,292,689 - dprA LU632_RS22465 0.19 +0.4 4,292,708 + dprA LU632_RS22465 0.20 +0.3 4,292,708 + dprA LU632_RS22465 0.20 +2.0 4,292,709 - dprA LU632_RS22465 0.20 -0.6 4,292,709 - dprA LU632_RS22465 0.20 +0.8 4,292,710 + dprA LU632_RS22465 0.21 +1.4 4,292,772 + dprA LU632_RS22465 0.26 -0.7 4,292,773 - dprA LU632_RS22465 0.26 +0.2 4,292,821 - dprA LU632_RS22465 0.30 -1.1 4,292,930 - dprA LU632_RS22465 0.40 -3.3 4,292,930 - dprA LU632_RS22465 0.40 +0.0 4,292,930 - dprA LU632_RS22465 0.40 +0.6 4,292,962 + dprA LU632_RS22465 0.43 -0.2
Or see this region's nucleotide sequence