Experiment: plant=Squash; plant sample=QP1; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS08905 and LU632_RS08910 are separated by 87 nucleotides LU632_RS08910 and hisIE are separated by 322 nucleotides hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides
LU632_RS08905: LU632_RS08905 - hypothetical protein, at 1,682,362 to 1,682,760
_RS08905
LU632_RS08910: LU632_RS08910 - hypothetical protein, at 1,682,848 to 1,683,159
_RS08910
LU632_RS08915: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 1,683,482 to 1,684,093
hisIE
LU632_RS08920: hisF - imidazole glycerol phosphate synthase subunit HisF, at 1,684,087 to 1,684,863
hisF
LU632_RS08925: hisA - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,684,845 to 1,685,582
hisA
Position (kb)
1683
1684
1685 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1682.571 kb on - strand, within LU632_RS08905 at 1682.592 kb on + strand, within LU632_RS08905 at 1682.614 kb on + strand, within LU632_RS08905 at 1682.615 kb on - strand, within LU632_RS08905 at 1682.615 kb on - strand, within LU632_RS08905 at 1682.678 kb on + strand, within LU632_RS08905 at 1682.747 kb on + strand at 1682.747 kb on + strand at 1682.748 kb on - strand at 1682.748 kb on - strand at 1682.751 kb on + strand at 1682.779 kb on - strand at 1682.779 kb on - strand at 1682.901 kb on + strand, within LU632_RS08910 at 1682.911 kb on + strand, within LU632_RS08910 at 1682.911 kb on + strand, within LU632_RS08910 at 1682.911 kb on + strand, within LU632_RS08910 at 1682.911 kb on + strand, within LU632_RS08910 at 1682.911 kb on + strand, within LU632_RS08910 at 1682.912 kb on - strand, within LU632_RS08910 at 1682.912 kb on - strand, within LU632_RS08910 at 1682.912 kb on - strand, within LU632_RS08910 at 1683.066 kb on + strand, within LU632_RS08910 at 1683.066 kb on + strand, within LU632_RS08910 at 1683.079 kb on - strand, within LU632_RS08910 at 1683.106 kb on - strand, within LU632_RS08910 at 1683.191 kb on - strand at 1683.211 kb on + strand at 1683.212 kb on - strand at 1683.261 kb on + strand at 1683.269 kb on + strand at 1683.313 kb on - strand at 1683.318 kb on - strand at 1683.355 kb on + strand at 1683.385 kb on - strand at 1683.441 kb on + strand at 1683.442 kb on - strand at 1683.448 kb on - strand at 1683.473 kb on + strand at 1683.561 kb on + strand, within hisIE at 1683.562 kb on - strand, within hisIE at 1683.562 kb on - strand, within hisIE at 1683.985 kb on + strand, within hisIE at 1683.985 kb on + strand, within hisIE at 1683.986 kb on - strand, within hisIE at 1683.987 kb on + strand, within hisIE at 1683.987 kb on + strand, within hisIE at 1683.988 kb on - strand, within hisIE at 1684.042 kb on + strand at 1684.043 kb on - strand at 1684.146 kb on + strand at 1684.146 kb on + strand at 1684.146 kb on + strand at 1684.149 kb on + strand at 1684.263 kb on + strand, within hisF at 1684.263 kb on + strand, within hisF at 1684.263 kb on + strand, within hisF at 1684.264 kb on - strand, within hisF at 1684.332 kb on + strand, within hisF at 1684.332 kb on + strand, within hisF at 1684.336 kb on - strand, within hisF at 1684.431 kb on - strand, within hisF at 1684.445 kb on + strand, within hisF at 1684.466 kb on + strand, within hisF at 1684.466 kb on + strand, within hisF at 1684.467 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.589 kb on - strand, within hisF at 1684.801 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand at 1684.866 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP1; plant location=NA remove 1,682,571 - LU632_RS08905 0.52 -0.6 1,682,592 + LU632_RS08905 0.58 +2.2 1,682,614 + LU632_RS08905 0.63 +2.1 1,682,615 - LU632_RS08905 0.63 +0.0 1,682,615 - LU632_RS08905 0.63 -1.5 1,682,678 + LU632_RS08905 0.79 -1.6 1,682,747 + -1.3 1,682,747 + -0.7 1,682,748 - +0.4 1,682,748 - +0.1 1,682,751 + +0.6 1,682,779 - +0.2 1,682,779 - -2.3 1,682,901 + LU632_RS08910 0.17 -1.2 1,682,911 + LU632_RS08910 0.20 -0.7 1,682,911 + LU632_RS08910 0.20 +1.4 1,682,911 + LU632_RS08910 0.20 +0.0 1,682,911 + LU632_RS08910 0.20 +0.8 1,682,911 + LU632_RS08910 0.20 -2.0 1,682,912 - LU632_RS08910 0.21 +0.1 1,682,912 - LU632_RS08910 0.21 +0.5 1,682,912 - LU632_RS08910 0.21 +2.2 1,683,066 + LU632_RS08910 0.70 -1.1 1,683,066 + LU632_RS08910 0.70 +0.5 1,683,079 - LU632_RS08910 0.74 -1.3 1,683,106 - LU632_RS08910 0.83 +0.7 1,683,191 - +0.7 1,683,211 + -0.2 1,683,212 - +0.8 1,683,261 + -0.4 1,683,269 + -0.0 1,683,313 - -0.2 1,683,318 - +3.1 1,683,355 + +1.1 1,683,385 - +0.5 1,683,441 + -1.6 1,683,442 - -0.9 1,683,448 - +2.0 1,683,473 + -1.2 1,683,561 + hisIE LU632_RS08915 0.13 -0.9 1,683,562 - hisIE LU632_RS08915 0.13 -2.5 1,683,562 - hisIE LU632_RS08915 0.13 +0.4 1,683,985 + hisIE LU632_RS08915 0.82 -2.8 1,683,985 + hisIE LU632_RS08915 0.82 -2.5 1,683,986 - hisIE LU632_RS08915 0.82 -0.3 1,683,987 + hisIE LU632_RS08915 0.83 -1.3 1,683,987 + hisIE LU632_RS08915 0.83 -2.2 1,683,988 - hisIE LU632_RS08915 0.83 -2.7 1,684,042 + -3.0 1,684,043 - -1.7 1,684,146 + -0.9 1,684,146 + -2.1 1,684,146 + -0.8 1,684,149 + -0.7 1,684,263 + hisF LU632_RS08920 0.23 -3.7 1,684,263 + hisF LU632_RS08920 0.23 -0.3 1,684,263 + hisF LU632_RS08920 0.23 +0.7 1,684,264 - hisF LU632_RS08920 0.23 -1.9 1,684,332 + hisF LU632_RS08920 0.32 -2.7 1,684,332 + hisF LU632_RS08920 0.32 -1.8 1,684,336 - hisF LU632_RS08920 0.32 -1.4 1,684,431 - hisF LU632_RS08920 0.44 -2.0 1,684,445 + hisF LU632_RS08920 0.46 -2.7 1,684,466 + hisF LU632_RS08920 0.49 -4.1 1,684,466 + hisF LU632_RS08920 0.49 -1.5 1,684,467 - hisF LU632_RS08920 0.49 +0.3 1,684,589 - hisF LU632_RS08920 0.65 -1.2 1,684,589 - hisF LU632_RS08920 0.65 +0.3 1,684,589 - hisF LU632_RS08920 0.65 +0.1 1,684,801 + -1.5 1,684,866 + -0.9 1,684,866 + -0.9 1,684,866 + +0.1
Or see this region's nucleotide sequence