Experiment: plant=Squash; plant sample=QP1; plant location=NA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt crl and LU632_RS01175 are separated by 79 nucleotides LU632_RS01175 and LU632_RS01180 are separated by 48 nucleotides LU632_RS01180 and proB are separated by 321 nucleotides proB and proA are separated by 15 nucleotides
LU632_RS01170: crl - sigma factor-binding protein Crl, at 249,053 to 249,454
crl
LU632_RS01175: LU632_RS01175 - porin, at 249,534 to 249,732
_RS01175
LU632_RS01180: LU632_RS01180 - hypothetical protein, at 249,781 to 249,870
_RS01180
LU632_RS01185: proB - glutamate 5-kinase, at 250,192 to 251,295
proB
LU632_RS01190: proA - glutamate-5-semialdehyde dehydrogenase, at 251,311 to 252,564
proA
Position (kb) 250
251
252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 249.257 kb on + strand, within crl at 249.258 kb on - strand, within crl at 249.263 kb on + strand, within crl at 249.263 kb on + strand, within crl at 249.264 kb on - strand, within crl at 249.267 kb on - strand, within crl at 249.279 kb on - strand, within crl at 249.355 kb on + strand, within crl at 249.355 kb on + strand, within crl at 249.356 kb on - strand, within crl at 249.356 kb on - strand, within crl at 249.401 kb on - strand, within crl at 249.509 kb on + strand at 249.510 kb on - strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.528 kb on - strand at 249.652 kb on + strand, within LU632_RS01175 at 249.653 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.687 kb on - strand, within LU632_RS01175 at 249.731 kb on + strand at 249.785 kb on + strand at 249.785 kb on + strand at 249.810 kb on + strand, within LU632_RS01180 at 250.021 kb on - strand at 250.149 kb on + strand at 250.149 kb on + strand at 250.163 kb on + strand at 250.164 kb on - strand at 250.170 kb on - strand at 250.174 kb on - strand at 250.238 kb on + strand at 250.272 kb on + strand at 250.273 kb on - strand at 250.273 kb on - strand at 250.273 kb on - strand at 250.416 kb on - strand, within proB at 250.472 kb on - strand, within proB at 250.482 kb on + strand, within proB at 250.482 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.485 kb on - strand, within proB at 250.485 kb on - strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.554 kb on - strand, within proB at 250.575 kb on + strand, within proB at 250.575 kb on + strand, within proB at 250.576 kb on - strand, within proB at 250.576 kb on - strand, within proB at 250.809 kb on - strand, within proB at 250.884 kb on + strand, within proB at 250.885 kb on - strand, within proB at 250.885 kb on - strand, within proB at 250.986 kb on + strand, within proB at 251.182 kb on - strand, within proB at 251.221 kb on - strand at 251.245 kb on - strand at 251.281 kb on - strand at 251.529 kb on - strand, within proA at 251.666 kb on - strand, within proA at 251.685 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.871 kb on - strand, within proA at 251.871 kb on - strand, within proA at 251.874 kb on + strand, within proA at 251.874 kb on + strand, within proA at 251.874 kb on + strand, within proA at 251.875 kb on - strand, within proA at 251.875 kb on - strand, within proA at 252.039 kb on + strand, within proA at 252.040 kb on - strand, within proA at 252.040 kb on - strand, within proA
Per-strain Table
Position Strand Gene LocusTag Fraction plant=Squash; plant sample=QP1; plant location=NA remove 249,257 + crl LU632_RS01170 0.51 +0.3 249,258 - crl LU632_RS01170 0.51 -0.2 249,263 + crl LU632_RS01170 0.52 -2.2 249,263 + crl LU632_RS01170 0.52 -1.2 249,264 - crl LU632_RS01170 0.52 +0.5 249,267 - crl LU632_RS01170 0.53 -0.3 249,279 - crl LU632_RS01170 0.56 +0.7 249,355 + crl LU632_RS01170 0.75 -0.7 249,355 + crl LU632_RS01170 0.75 +0.3 249,356 - crl LU632_RS01170 0.75 -0.1 249,356 - crl LU632_RS01170 0.75 +1.5 249,401 - crl LU632_RS01170 0.87 +0.6 249,509 + +4.1 249,510 - -0.3 249,519 + +0.2 249,519 + +2.4 249,519 + -1.9 249,519 + +0.3 249,520 - +0.3 249,520 - +0.0 249,520 - +0.6 249,520 - -0.7 249,528 - -0.0 249,652 + LU632_RS01175 0.59 +0.5 249,653 - LU632_RS01175 0.60 +0.3 249,656 - LU632_RS01175 0.61 -0.9 249,656 - LU632_RS01175 0.61 +0.2 249,656 - LU632_RS01175 0.61 +0.3 249,676 + LU632_RS01175 0.71 +0.2 249,676 + LU632_RS01175 0.71 +0.9 249,676 + LU632_RS01175 0.71 +1.9 249,677 - LU632_RS01175 0.72 +1.6 249,677 - LU632_RS01175 0.72 +0.3 249,677 - LU632_RS01175 0.72 -0.1 249,687 - LU632_RS01175 0.77 +1.0 249,731 + -0.3 249,785 + +0.6 249,785 + -0.3 249,810 + LU632_RS01180 0.32 +0.1 250,021 - -0.1 250,149 + -2.6 250,149 + +0.5 250,163 + +1.7 250,164 - +0.2 250,170 - -2.3 250,174 - -0.6 250,238 + -0.4 250,272 + +0.3 250,273 - -2.4 250,273 - -0.2 250,273 - -0.1 250,416 - proB LU632_RS01185 0.20 -1.3 250,472 - proB LU632_RS01185 0.25 -0.4 250,482 + proB LU632_RS01185 0.26 -1.1 250,482 + proB LU632_RS01185 0.26 -0.4 250,484 + proB LU632_RS01185 0.26 -2.2 250,484 + proB LU632_RS01185 0.26 -0.1 250,485 - proB LU632_RS01185 0.27 -1.9 250,485 - proB LU632_RS01185 0.27 -0.7 250,553 + proB LU632_RS01185 0.33 -3.5 250,553 + proB LU632_RS01185 0.33 -1.5 250,553 + proB LU632_RS01185 0.33 -1.6 250,554 - proB LU632_RS01185 0.33 -1.9 250,575 + proB LU632_RS01185 0.35 -0.5 250,575 + proB LU632_RS01185 0.35 -2.4 250,576 - proB LU632_RS01185 0.35 +0.9 250,576 - proB LU632_RS01185 0.35 -2.1 250,809 - proB LU632_RS01185 0.56 -2.3 250,884 + proB LU632_RS01185 0.63 -2.0 250,885 - proB LU632_RS01185 0.63 -1.8 250,885 - proB LU632_RS01185 0.63 -0.7 250,986 + proB LU632_RS01185 0.72 +1.5 251,182 - proB LU632_RS01185 0.90 -0.7 251,221 - -2.5 251,245 - -1.1 251,281 - -0.4 251,529 - proA LU632_RS01190 0.17 -2.2 251,666 - proA LU632_RS01190 0.28 -0.8 251,685 + proA LU632_RS01190 0.30 -2.9 251,870 + proA LU632_RS01190 0.45 +1.5 251,870 + proA LU632_RS01190 0.45 +0.2 251,870 + proA LU632_RS01190 0.45 -1.1 251,870 + proA LU632_RS01190 0.45 -0.7 251,871 - proA LU632_RS01190 0.45 -2.0 251,871 - proA LU632_RS01190 0.45 -0.9 251,874 + proA LU632_RS01190 0.45 -0.9 251,874 + proA LU632_RS01190 0.45 -1.6 251,874 + proA LU632_RS01190 0.45 -3.2 251,875 - proA LU632_RS01190 0.45 -1.5 251,875 - proA LU632_RS01190 0.45 +0.3 252,039 + proA LU632_RS01190 0.58 -1.2 252,040 - proA LU632_RS01190 0.58 -0.1 252,040 - proA LU632_RS01190 0.58 -2.6
Or see this region's nucleotide sequence