Strain Fitness in Escherichia coli BL21 around ECD_03965

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03963 and ECD_03964 are separated by 146 nucleotidesECD_03964 and ECD_03965 are separated by 1 nucleotidesECD_03965 and ECD_03966 overlap by 14 nucleotidesECD_03966 and ECD_03967 overlap by 1 nucleotides ECD_03963: ECD_03963 - putative periplasmic protein, at 4,221,055 to 4,221,384 _03963 ECD_03964: ECD_03964 - 5-phospho-alpha-D-ribosyl 1,2-cyclic phosphate phosphodiesterase, at 4,221,531 to 4,222,289 _03964 ECD_03965: ECD_03965 - aminoalkylphosphonate N-acetyltransferase, at 4,222,291 to 4,222,725 _03965 ECD_03966: ECD_03966 - ribose 1,5-bisphosphokinase, at 4,222,712 to 4,223,269 _03966 ECD_03967: ECD_03967 - ribophosphonate triphosphate hydrolase, at 4,223,269 to 4,224,405 _03967 Position (kb) 4222 4223Strain fitness (log2 ratio) -1 0 1at 4221.374 kb on - strandat 4221.374 kb on - strandat 4221.624 kb on - strand, within ECD_03964at 4222.055 kb on + strand, within ECD_03964at 4222.056 kb on - strand, within ECD_03964at 4222.056 kb on - strand, within ECD_03964at 4222.056 kb on - strand, within ECD_03964at 4222.152 kb on - strand, within ECD_03964at 4222.162 kb on - strand, within ECD_03964at 4222.293 kb on - strandat 4222.293 kb on - strandat 4222.474 kb on + strand, within ECD_03965at 4222.691 kb on + strandat 4222.692 kb on - strandat 4222.820 kb on - strand, within ECD_03966at 4222.820 kb on - strand, within ECD_03966at 4222.820 kb on - strand, within ECD_03966at 4223.041 kb on - strand, within ECD_03966at 4223.044 kb on - strand, within ECD_03966at 4223.313 kb on + strandat 4223.313 kb on + strandat 4223.313 kb on + strandat 4223.314 kb on - strandat 4223.314 kb on - strandat 4223.314 kb on - strandat 4223.314 kb on - strandat 4223.314 kb on - strandat 4223.501 kb on + strand, within ECD_03967

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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4,221,374 - +0.1
4,221,374 - -1.2
4,221,624 - ECD_03964 0.12 +0.6
4,222,055 + ECD_03964 0.69 +0.4
4,222,056 - ECD_03964 0.69 +0.6
4,222,056 - ECD_03964 0.69 +0.1
4,222,056 - ECD_03964 0.69 +0.5
4,222,152 - ECD_03964 0.82 +0.3
4,222,162 - ECD_03964 0.83 +0.4
4,222,293 - +0.0
4,222,293 - -0.5
4,222,474 + ECD_03965 0.42 +0.2
4,222,691 + +0.1
4,222,692 - +0.6
4,222,820 - ECD_03966 0.19 -0.6
4,222,820 - ECD_03966 0.19 -0.5
4,222,820 - ECD_03966 0.19 +0.5
4,223,041 - ECD_03966 0.59 -0.9
4,223,044 - ECD_03966 0.59 -0.4
4,223,313 + -0.1
4,223,313 + +1.2
4,223,313 + +0.9
4,223,314 - -0.0
4,223,314 - +0.1
4,223,314 - -0.7
4,223,314 - -0.4
4,223,314 - +0.1
4,223,501 + ECD_03967 0.20 -0.8

Or see this region's nucleotide sequence