Experiment: Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_03505 and ECD_03506 are separated by 54 nucleotides ECD_03506 and ECD_03507 are separated by 18 nucleotides ECD_03507 and ECD_03508 are separated by 6 nucleotides ECD_03508 and ECD_03509 are separated by 5 nucleotides
ECD_03505: ECD_03505 - guanylate kinase, at 3,688,220 to 3,688,843
_03505
ECD_03506: ECD_03506 - RNA polymerase, omega subunit, at 3,688,898 to 3,689,173
_03506
ECD_03507: ECD_03507 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 3,689,192 to 3,691,300
_03507
ECD_03508: ECD_03508 - tRNA mG18-2'-O-methyltransferase, SAM-dependent, at 3,691,307 to 3,691,996
_03508
ECD_03509: ECD_03509 - ATP-dependent DNA helicase, at 3,692,002 to 3,694,083
_03509
Position (kb)
3689
3690
3691
3692 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3688.206 kb on + strand at 3688.893 kb on + strand at 3688.893 kb on + strand at 3688.897 kb on + strand at 3688.908 kb on + strand at 3689.040 kb on + strand, within ECD_03506 at 3689.040 kb on + strand, within ECD_03506 at 3689.152 kb on + strand at 3690.356 kb on + strand, within ECD_03507 at 3690.356 kb on + strand, within ECD_03507 at 3690.871 kb on + strand, within ECD_03507 at 3690.872 kb on - strand, within ECD_03507 at 3691.027 kb on + strand, within ECD_03507 at 3691.027 kb on + strand, within ECD_03507 at 3691.132 kb on + strand at 3691.150 kb on + strand at 3691.165 kb on + strand at 3691.190 kb on + strand at 3691.276 kb on + strand at 3691.277 kb on - strand at 3691.306 kb on + strand at 3691.306 kb on + strand at 3691.306 kb on + strand at 3691.306 kb on + strand at 3691.307 kb on - strand at 3691.440 kb on + strand, within ECD_03508 at 3691.440 kb on + strand, within ECD_03508 at 3691.508 kb on - strand, within ECD_03508 at 3691.817 kb on + strand, within ECD_03508 at 3691.975 kb on + strand at 3691.978 kb on + strand at 3691.979 kb on - strand at 3691.979 kb on - strand at 3691.994 kb on + strand at 3691.994 kb on + strand at 3692.018 kb on + strand at 3692.018 kb on + strand at 3692.064 kb on + strand at 3692.064 kb on + strand at 3692.065 kb on - strand at 3692.114 kb on + strand at 3692.115 kb on - strand at 3692.168 kb on + strand at 3692.168 kb on + strand at 3692.169 kb on - strand at 3692.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas31 remove 3,688,206 + -0.0 3,688,893 + +0.0 3,688,893 + +0.7 3,688,897 + -0.4 3,688,908 + -0.3 3,689,040 + ECD_03506 0.51 -0.4 3,689,040 + ECD_03506 0.51 -1.7 3,689,152 + +0.8 3,690,356 + ECD_03507 0.55 -0.1 3,690,356 + ECD_03507 0.55 -3.2 3,690,871 + ECD_03507 0.80 -0.0 3,690,872 - ECD_03507 0.80 -0.4 3,691,027 + ECD_03507 0.87 -0.1 3,691,027 + ECD_03507 0.87 -0.1 3,691,132 + -0.5 3,691,150 + -0.8 3,691,165 + +0.0 3,691,190 + -0.8 3,691,276 + +0.6 3,691,277 - +0.6 3,691,306 + +1.4 3,691,306 + +0.4 3,691,306 + -0.0 3,691,306 + +0.1 3,691,307 - +0.1 3,691,440 + ECD_03508 0.19 -0.5 3,691,440 + ECD_03508 0.19 +0.8 3,691,508 - ECD_03508 0.29 +1.0 3,691,817 + ECD_03508 0.74 +1.8 3,691,975 + -0.5 3,691,978 + +0.8 3,691,979 - +1.0 3,691,979 - -0.9 3,691,994 + +0.8 3,691,994 + +0.2 3,692,018 + -1.5 3,692,018 + +0.2 3,692,064 + -0.3 3,692,064 + -1.2 3,692,065 - -1.3 3,692,114 + -1.1 3,692,115 - -0.2 3,692,168 + +0.5 3,692,168 + -1.0 3,692,169 - -0.2 3,692,182 + -0.7
Or see this region's nucleotide sequence