Strain Fitness in Escherichia coli BL21 around ECD_00030

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00028 and ECD_00029 are separated by 7 nucleotidesECD_00029 and ECD_00030 are separated by 42 nucleotidesECD_00030 and ECD_00031 overlap by 1 nucleotidesECD_00031 and ECD_00032 are separated by 124 nucleotides ECD_00028: ECD_00028 - uncharacterized protein, at 25,253 to 25,471 _00028 ECD_00029: ECD_00029 - bifunctional riboflavin kinase/FAD synthetase, at 25,479 to 26,420 _00029 ECD_00030: ECD_00030 - isoleucyl-tRNA synthetase, at 26,463 to 29,279 _00030 ECD_00031: ECD_00031 - prolipoprotein signal peptidase (signal peptidase II), at 29,279 to 29,773 _00031 ECD_00032: ECD_00032 - FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase), at 29,898 to 30,347 _00032 Position (kb) 26 27 28 29 30Strain fitness (log2 ratio) -1 0 1at 26.427 kb on + strandat 26.427 kb on + strandat 29.755 kb on + strandat 29.771 kb on + strandat 29.774 kb on + strandat 29.774 kb on + strandat 29.853 kb on + strandat 29.881 kb on + strandat 30.138 kb on + strand, within ECD_00032at 30.138 kb on + strand, within ECD_00032

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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26,427 + -0.5
26,427 + -0.2
29,755 + +0.6
29,771 + +0.4
29,774 + +0.8
29,774 + -0.7
29,853 + -1.3
29,881 + -0.4
30,138 + ECD_00032 0.53 +1.3
30,138 + ECD_00032 0.53 -0.4

Or see this region's nucleotide sequence