Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS18115 and ABIE41_RS18120 are separated by 2 nucleotides ABIE41_RS18120 and ABIE41_RS18125 are separated by 5 nucleotides ABIE41_RS18125 and ABIE41_RS18130 overlap by 4 nucleotides
ABIE41_RS18115: ABIE41_RS18115 - urea ABC transporter ATP-binding subunit UrtE, at 3,730,907 to 3,731,602
_RS18115
ABIE41_RS18120: ABIE41_RS18120 - urea ABC transporter ATP-binding protein UrtD, at 3,731,605 to 3,732,369
_RS18120
ABIE41_RS18125: ABIE41_RS18125 - urea ABC transporter permease subunit UrtC, at 3,732,375 to 3,733,514
_RS18125
ABIE41_RS18130: ABIE41_RS18130 - urea ABC transporter permease subunit UrtB, at 3,733,511 to 3,735,112
_RS18130
Position (kb)
3732
3733
3734 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3731.571 kb on + strand at 3731.618 kb on + strand at 3731.619 kb on - strand at 3731.730 kb on + strand, within ABIE41_RS18120 at 3732.326 kb on + strand at 3732.326 kb on + strand at 3732.327 kb on - strand at 3732.327 kb on - strand at 3732.327 kb on - strand at 3732.327 kb on - strand at 3732.472 kb on + strand at 3732.556 kb on + strand, within ABIE41_RS18125 at 3732.556 kb on + strand, within ABIE41_RS18125 at 3732.685 kb on - strand, within ABIE41_RS18125 at 3732.685 kb on - strand, within ABIE41_RS18125 at 3732.688 kb on + strand, within ABIE41_RS18125 at 3732.688 kb on + strand, within ABIE41_RS18125 at 3732.688 kb on + strand, within ABIE41_RS18125 at 3732.688 kb on + strand, within ABIE41_RS18125 at 3732.688 kb on + strand, within ABIE41_RS18125 at 3732.688 kb on + strand, within ABIE41_RS18125 at 3732.688 kb on + strand, within ABIE41_RS18125 at 3732.689 kb on - strand, within ABIE41_RS18125 at 3732.689 kb on - strand, within ABIE41_RS18125 at 3733.004 kb on - strand, within ABIE41_RS18125 at 3733.142 kb on - strand, within ABIE41_RS18125 at 3733.207 kb on + strand, within ABIE41_RS18125 at 3733.207 kb on + strand, within ABIE41_RS18125 at 3733.207 kb on + strand, within ABIE41_RS18125 at 3733.208 kb on - strand, within ABIE41_RS18125 at 3733.208 kb on - strand, within ABIE41_RS18125 at 3733.208 kb on - strand, within ABIE41_RS18125 at 3733.208 kb on - strand, within ABIE41_RS18125 at 3733.208 kb on - strand, within ABIE41_RS18125 at 3733.208 kb on - strand, within ABIE41_RS18125 at 3733.240 kb on + strand, within ABIE41_RS18125 at 3733.240 kb on + strand, within ABIE41_RS18125 at 3733.240 kb on + strand, within ABIE41_RS18125 at 3733.240 kb on + strand, within ABIE41_RS18125 at 3733.240 kb on + strand, within ABIE41_RS18125 at 3733.240 kb on + strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.241 kb on - strand, within ABIE41_RS18125 at 3733.306 kb on + strand, within ABIE41_RS18125 at 3733.345 kb on + strand, within ABIE41_RS18125 at 3733.345 kb on + strand, within ABIE41_RS18125 at 3733.346 kb on - strand, within ABIE41_RS18125 at 3733.354 kb on + strand, within ABIE41_RS18125 at 3733.355 kb on - strand, within ABIE41_RS18125 at 3733.355 kb on - strand, within ABIE41_RS18125 at 3733.620 kb on + strand at 3733.620 kb on + strand at 3733.728 kb on + strand, within ABIE41_RS18130 at 3733.728 kb on + strand, within ABIE41_RS18130 at 3733.728 kb on + strand, within ABIE41_RS18130 at 3733.728 kb on + strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3733.729 kb on - strand, within ABIE41_RS18130 at 3734.127 kb on + strand, within ABIE41_RS18130 at 3734.214 kb on + strand, within ABIE41_RS18130 at 3734.214 kb on + strand, within ABIE41_RS18130 at 3734.215 kb on - strand, within ABIE41_RS18130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM remove 3,731,571 + -0.1 3,731,618 + +0.1 3,731,619 - -0.7 3,731,730 + ABIE41_RS18120 0.16 -0.7 3,732,326 + +0.4 3,732,326 + +0.3 3,732,327 - +0.8 3,732,327 - +0.6 3,732,327 - +3.4 3,732,327 - -1.0 3,732,472 + +0.6 3,732,556 + ABIE41_RS18125 0.16 -0.3 3,732,556 + ABIE41_RS18125 0.16 -0.1 3,732,685 - ABIE41_RS18125 0.27 -1.1 3,732,685 - ABIE41_RS18125 0.27 -0.1 3,732,688 + ABIE41_RS18125 0.27 -0.2 3,732,688 + ABIE41_RS18125 0.27 +0.6 3,732,688 + ABIE41_RS18125 0.27 +1.2 3,732,688 + ABIE41_RS18125 0.27 -0.2 3,732,688 + ABIE41_RS18125 0.27 +0.3 3,732,688 + ABIE41_RS18125 0.27 +0.6 3,732,688 + ABIE41_RS18125 0.27 -0.2 3,732,689 - ABIE41_RS18125 0.28 +0.6 3,732,689 - ABIE41_RS18125 0.28 -0.1 3,733,004 - ABIE41_RS18125 0.55 +0.7 3,733,142 - ABIE41_RS18125 0.67 +0.5 3,733,207 + ABIE41_RS18125 0.73 -0.1 3,733,207 + ABIE41_RS18125 0.73 -0.9 3,733,207 + ABIE41_RS18125 0.73 +0.0 3,733,208 - ABIE41_RS18125 0.73 +1.3 3,733,208 - ABIE41_RS18125 0.73 -0.1 3,733,208 - ABIE41_RS18125 0.73 +1.8 3,733,208 - ABIE41_RS18125 0.73 -0.7 3,733,208 - ABIE41_RS18125 0.73 -1.3 3,733,208 - ABIE41_RS18125 0.73 +0.8 3,733,240 + ABIE41_RS18125 0.76 +0.4 3,733,240 + ABIE41_RS18125 0.76 +0.1 3,733,240 + ABIE41_RS18125 0.76 -1.4 3,733,240 + ABIE41_RS18125 0.76 +2.4 3,733,240 + ABIE41_RS18125 0.76 -0.1 3,733,240 + ABIE41_RS18125 0.76 -0.8 3,733,241 - ABIE41_RS18125 0.76 -0.1 3,733,241 - ABIE41_RS18125 0.76 +0.2 3,733,241 - ABIE41_RS18125 0.76 -0.0 3,733,241 - ABIE41_RS18125 0.76 -0.1 3,733,241 - ABIE41_RS18125 0.76 -0.5 3,733,241 - ABIE41_RS18125 0.76 -1.0 3,733,241 - ABIE41_RS18125 0.76 +0.0 3,733,241 - ABIE41_RS18125 0.76 +0.1 3,733,306 + ABIE41_RS18125 0.82 +0.1 3,733,345 + ABIE41_RS18125 0.85 -0.2 3,733,345 + ABIE41_RS18125 0.85 +0.1 3,733,346 - ABIE41_RS18125 0.85 +0.5 3,733,354 + ABIE41_RS18125 0.86 -0.2 3,733,355 - ABIE41_RS18125 0.86 +1.4 3,733,355 - ABIE41_RS18125 0.86 -0.1 3,733,620 + -0.2 3,733,620 + -0.9 3,733,728 + ABIE41_RS18130 0.14 -0.2 3,733,728 + ABIE41_RS18130 0.14 +0.4 3,733,728 + ABIE41_RS18130 0.14 +0.2 3,733,728 + ABIE41_RS18130 0.14 -0.3 3,733,729 - ABIE41_RS18130 0.14 +1.0 3,733,729 - ABIE41_RS18130 0.14 -0.4 3,733,729 - ABIE41_RS18130 0.14 -0.2 3,733,729 - ABIE41_RS18130 0.14 +0.3 3,733,729 - ABIE41_RS18130 0.14 +0.4 3,733,729 - ABIE41_RS18130 0.14 -0.2 3,733,729 - ABIE41_RS18130 0.14 -0.6 3,733,729 - ABIE41_RS18130 0.14 -0.3 3,734,127 + ABIE41_RS18130 0.38 +0.6 3,734,214 + ABIE41_RS18130 0.44 +1.6 3,734,214 + ABIE41_RS18130 0.44 -0.6 3,734,215 - ABIE41_RS18130 0.44 -1.2
Or see this region's nucleotide sequence