Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS08530 and ABIE41_RS08535 are separated by 332 nucleotides ABIE41_RS08535 and ABIE41_RS08540 overlap by 96 nucleotides
ABIE41_RS08530: ABIE41_RS08530 - N-formylglutamate amidohydrolase, at 1,759,283 to 1,760,203
_RS08530
ABIE41_RS08535: ABIE41_RS08535 - histidinol-phosphatase, at 1,760,536 to 1,761,318
_RS08535
ABIE41_RS08540: ABIE41_RS08540 - alpha/beta hydrolase, at 1,761,223 to 1,762,290
_RS08540
Position (kb)
1760
1761
1762 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1759.580 kb on + strand, within ABIE41_RS08530 at 1759.581 kb on - strand, within ABIE41_RS08530 at 1759.581 kb on - strand, within ABIE41_RS08530 at 1759.700 kb on + strand, within ABIE41_RS08530 at 1759.700 kb on + strand, within ABIE41_RS08530 at 1759.700 kb on + strand, within ABIE41_RS08530 at 1759.700 kb on + strand, within ABIE41_RS08530 at 1759.700 kb on + strand, within ABIE41_RS08530 at 1759.700 kb on + strand, within ABIE41_RS08530 at 1759.701 kb on - strand, within ABIE41_RS08530 at 1759.701 kb on - strand, within ABIE41_RS08530 at 1759.701 kb on - strand, within ABIE41_RS08530 at 1759.701 kb on - strand, within ABIE41_RS08530 at 1759.752 kb on - strand, within ABIE41_RS08530 at 1759.774 kb on - strand, within ABIE41_RS08530 at 1759.922 kb on + strand, within ABIE41_RS08530 at 1759.923 kb on - strand, within ABIE41_RS08530 at 1759.923 kb on - strand, within ABIE41_RS08530 at 1759.988 kb on + strand, within ABIE41_RS08530 at 1759.989 kb on - strand, within ABIE41_RS08530 at 1759.989 kb on - strand, within ABIE41_RS08530 at 1760.015 kb on + strand, within ABIE41_RS08530 at 1760.015 kb on - strand, within ABIE41_RS08530 at 1760.016 kb on - strand, within ABIE41_RS08530 at 1760.072 kb on + strand, within ABIE41_RS08530 at 1760.073 kb on - strand, within ABIE41_RS08530 at 1760.201 kb on + strand at 1760.201 kb on + strand at 1760.202 kb on - strand at 1760.347 kb on + strand at 1760.347 kb on + strand at 1760.348 kb on - strand at 1760.773 kb on + strand, within ABIE41_RS08535 at 1760.777 kb on - strand, within ABIE41_RS08535 at 1760.878 kb on + strand, within ABIE41_RS08535 at 1760.878 kb on + strand, within ABIE41_RS08535 at 1760.941 kb on + strand, within ABIE41_RS08535 at 1760.942 kb on - strand, within ABIE41_RS08535 at 1761.055 kb on + strand, within ABIE41_RS08535 at 1761.056 kb on - strand, within ABIE41_RS08535 at 1761.056 kb on - strand, within ABIE41_RS08535 at 1761.106 kb on + strand, within ABIE41_RS08535 at 1761.107 kb on - strand, within ABIE41_RS08535 at 1761.112 kb on + strand, within ABIE41_RS08535 at 1761.112 kb on + strand, within ABIE41_RS08535 at 1761.112 kb on + strand, within ABIE41_RS08535 at 1761.113 kb on - strand, within ABIE41_RS08535 at 1761.113 kb on - strand, within ABIE41_RS08535 at 1761.169 kb on + strand, within ABIE41_RS08535 at 1761.258 kb on + strand at 1761.360 kb on + strand, within ABIE41_RS08540 at 1761.360 kb on + strand, within ABIE41_RS08540 at 1761.360 kb on + strand, within ABIE41_RS08540 at 1761.360 kb on + strand, within ABIE41_RS08540 at 1761.360 kb on + strand, within ABIE41_RS08540 at 1761.361 kb on - strand, within ABIE41_RS08540 at 1761.361 kb on - strand, within ABIE41_RS08540 at 1761.668 kb on + strand, within ABIE41_RS08540 at 1761.669 kb on - strand, within ABIE41_RS08540 at 1761.669 kb on - strand, within ABIE41_RS08540 at 1761.669 kb on - strand, within ABIE41_RS08540 at 1761.926 kb on + strand, within ABIE41_RS08540 at 1761.926 kb on + strand, within ABIE41_RS08540 at 1761.926 kb on + strand, within ABIE41_RS08540 at 1761.927 kb on - strand, within ABIE41_RS08540 at 1761.983 kb on + strand, within ABIE41_RS08540 at 1761.984 kb on - strand, within ABIE41_RS08540 at 1761.984 kb on - strand, within ABIE41_RS08540 at 1762.256 kb on + strand at 1762.257 kb on - strand at 1762.257 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM remove 1,759,580 + ABIE41_RS08530 0.32 +2.9 1,759,581 - ABIE41_RS08530 0.32 +1.5 1,759,581 - ABIE41_RS08530 0.32 -0.3 1,759,700 + ABIE41_RS08530 0.45 +0.8 1,759,700 + ABIE41_RS08530 0.45 -0.3 1,759,700 + ABIE41_RS08530 0.45 +1.9 1,759,700 + ABIE41_RS08530 0.45 +0.9 1,759,700 + ABIE41_RS08530 0.45 +1.3 1,759,700 + ABIE41_RS08530 0.45 +0.4 1,759,701 - ABIE41_RS08530 0.45 +0.3 1,759,701 - ABIE41_RS08530 0.45 +0.0 1,759,701 - ABIE41_RS08530 0.45 -1.4 1,759,701 - ABIE41_RS08530 0.45 +0.1 1,759,752 - ABIE41_RS08530 0.51 +0.9 1,759,774 - ABIE41_RS08530 0.53 -0.1 1,759,922 + ABIE41_RS08530 0.69 +0.4 1,759,923 - ABIE41_RS08530 0.69 +0.3 1,759,923 - ABIE41_RS08530 0.69 +0.1 1,759,988 + ABIE41_RS08530 0.77 +0.7 1,759,989 - ABIE41_RS08530 0.77 +0.0 1,759,989 - ABIE41_RS08530 0.77 +0.0 1,760,015 + ABIE41_RS08530 0.79 -0.5 1,760,015 - ABIE41_RS08530 0.79 -0.0 1,760,016 - ABIE41_RS08530 0.80 +0.3 1,760,072 + ABIE41_RS08530 0.86 -2.5 1,760,073 - ABIE41_RS08530 0.86 +0.6 1,760,201 + +0.4 1,760,201 + -0.7 1,760,202 - +0.1 1,760,347 + +0.2 1,760,347 + +0.5 1,760,348 - +1.3 1,760,773 + ABIE41_RS08535 0.30 -0.1 1,760,777 - ABIE41_RS08535 0.31 +0.5 1,760,878 + ABIE41_RS08535 0.44 +0.7 1,760,878 + ABIE41_RS08535 0.44 +0.4 1,760,941 + ABIE41_RS08535 0.52 +1.1 1,760,942 - ABIE41_RS08535 0.52 +0.6 1,761,055 + ABIE41_RS08535 0.66 -0.0 1,761,056 - ABIE41_RS08535 0.66 +3.5 1,761,056 - ABIE41_RS08535 0.66 -0.0 1,761,106 + ABIE41_RS08535 0.73 +0.6 1,761,107 - ABIE41_RS08535 0.73 +0.7 1,761,112 + ABIE41_RS08535 0.74 +0.4 1,761,112 + ABIE41_RS08535 0.74 +1.0 1,761,112 + ABIE41_RS08535 0.74 +0.7 1,761,113 - ABIE41_RS08535 0.74 +0.1 1,761,113 - ABIE41_RS08535 0.74 +0.0 1,761,169 + ABIE41_RS08535 0.81 +2.1 1,761,258 + +0.7 1,761,360 + ABIE41_RS08540 0.13 +1.3 1,761,360 + ABIE41_RS08540 0.13 +0.6 1,761,360 + ABIE41_RS08540 0.13 +0.5 1,761,360 + ABIE41_RS08540 0.13 +0.8 1,761,360 + ABIE41_RS08540 0.13 +0.6 1,761,361 - ABIE41_RS08540 0.13 +1.7 1,761,361 - ABIE41_RS08540 0.13 +0.4 1,761,668 + ABIE41_RS08540 0.42 +0.8 1,761,669 - ABIE41_RS08540 0.42 +1.1 1,761,669 - ABIE41_RS08540 0.42 -2.1 1,761,669 - ABIE41_RS08540 0.42 +0.0 1,761,926 + ABIE41_RS08540 0.66 +1.3 1,761,926 + ABIE41_RS08540 0.66 +0.1 1,761,926 + ABIE41_RS08540 0.66 -0.2 1,761,927 - ABIE41_RS08540 0.66 +1.4 1,761,983 + ABIE41_RS08540 0.71 -1.0 1,761,984 - ABIE41_RS08540 0.71 +0.5 1,761,984 - ABIE41_RS08540 0.71 -0.3 1,762,256 + -0.5 1,762,257 - +0.2 1,762,257 - +0.4
Or see this region's nucleotide sequence