Strain Fitness in Escherichia coli BL21 around ECD_01749

Experiment: Bas29

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01748 and ECD_01749 are separated by 83 nucleotidesECD_01749 and ECD_01750 are separated by 47 nucleotidesECD_01750 and ECD_01751 are separated by 89 nucleotides ECD_01748: ECD_01748 - glyceraldehyde-3-phosphate dehydrogenase A, at 1,807,148 to 1,808,143 _01748 ECD_01749: ECD_01749 - D-hexose-6-phosphate epimerase-like protein, at 1,808,227 to 1,809,111 _01749 ECD_01750: ECD_01750 - aldo-keto reductase, methylglyoxal to acetol, NADPH-dependent, at 1,809,159 to 1,810,013 _01750 ECD_01751: ECD_01751 - scaffolding protein for murein synthesizing machinery, at 1,810,103 to 1,810,849 _01751 Position (kb) 1808 1809 1810Strain fitness (log2 ratio) -2 -1 0 1at 1808.442 kb on + strand, within ECD_01749at 1808.718 kb on + strand, within ECD_01749at 1809.023 kb on + strandat 1809.337 kb on - strand, within ECD_01750at 1809.947 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas29
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1,808,442 + ECD_01749 0.24 -2.0
1,808,718 + ECD_01749 0.55 -0.3
1,809,023 + +0.4
1,809,337 - ECD_01750 0.21 +0.4
1,809,947 - -0.1

Or see this region's nucleotide sequence