Strain Fitness in Escherichia coli BL21 around ECD_01721

Experiment: Bas29

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01719 and ECD_01721 are separated by 659 nucleotidesECD_01721 and ECD_01722 overlap by 1 nucleotidesECD_01722 and ECD_01723 are separated by 9 nucleotides ECD_01719: ECD_01719 - TVP38/TMEM64 family inner membrane protein, at 1,777,814 to 1,778,524 _01719 ECD_01721: ECD_01721 - TVP38/TMEM64 family inner membrane protein, at 1,779,184 to 1,779,891 _01721 ECD_01722: ECD_01722 - carboxymuconolactone decarboxylase family protein, at 1,779,891 to 1,780,439 _01722 ECD_01723: ECD_01723 - putative ABC transporter periplasmic binding protein, at 1,780,449 to 1,781,615 _01723 Position (kb) 1779 1780Strain fitness (log2 ratio) -1 0 1 2at 1778.426 kb on - strand, within ECD_01719at 1778.500 kb on + strandat 1778.522 kb on + strandat 1778.656 kb on + strandat 1778.716 kb on + strandat 1779.059 kb on + strandat 1779.065 kb on + strandat 1779.291 kb on + strand, within ECD_01721at 1779.757 kb on + strand, within ECD_01721

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas29
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1,778,426 - ECD_01719 0.86 +0.0
1,778,500 + -0.3
1,778,522 + +2.2
1,778,656 + +0.7
1,778,716 + +0.5
1,779,059 + -0.6
1,779,065 + -0.1
1,779,291 + ECD_01721 0.15 -1.4
1,779,757 + ECD_01721 0.81 -1.0

Or see this region's nucleotide sequence