Strain Fitness in Escherichia coli BL21 around ECD_00334

Experiment: Bas29

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00332 and ECD_00333 are separated by 16 nucleotidesECD_00333 and ECD_00334 are separated by 119 nucleotidesECD_00334 and ECD_00335 are separated by 182 nucleotidesECD_00335 and ECD_00336 are separated by 49 nucleotides ECD_00332: ECD_00332 - diguanylate cyclase, cellulose regualtor, at 368,328 to 369,443 _00332 ECD_00333: ECD_00333 - pyrroline-5-carboxylate reductase, NAD(P)-binding, at 369,460 to 370,269 _00333 ECD_00334: ECD_00334 - UPF0178 family protein, at 370,389 to 370,847 _00334 ECD_00335: ECD_00335 - shikimate kinase II, at 371,030 to 371,554 _00335 ECD_00336: ECD_00336 - OxyR-regulated conserved protein, at 371,604 to 371,795 _00336 Position (kb) 370 371Strain fitness (log2 ratio) -2 -1 0 1 2at 369.396 kb on + strandat 369.589 kb on - strand, within ECD_00333at 369.589 kb on - strand, within ECD_00333at 369.589 kb on - strand, within ECD_00333at 369.589 kb on - strand, within ECD_00333at 369.647 kb on - strand, within ECD_00333at 369.729 kb on + strand, within ECD_00333at 369.729 kb on + strand, within ECD_00333at 369.729 kb on + strand, within ECD_00333at 369.730 kb on - strand, within ECD_00333at 369.730 kb on - strand, within ECD_00333at 369.737 kb on + strand, within ECD_00333at 369.793 kb on + strand, within ECD_00333at 369.823 kb on - strand, within ECD_00333at 370.006 kb on - strand, within ECD_00333at 370.006 kb on - strand, within ECD_00333at 370.166 kb on + strand, within ECD_00333at 370.167 kb on - strand, within ECD_00333at 370.167 kb on - strand, within ECD_00333at 370.249 kb on - strandat 370.322 kb on + strandat 370.322 kb on + strandat 370.332 kb on + strandat 370.389 kb on - strandat 370.461 kb on - strand, within ECD_00334at 370.483 kb on + strand, within ECD_00334at 370.613 kb on + strand, within ECD_00334at 370.615 kb on + strand, within ECD_00334at 370.677 kb on + strand, within ECD_00334at 370.722 kb on - strand, within ECD_00334at 370.732 kb on + strand, within ECD_00334at 370.733 kb on - strand, within ECD_00334at 370.733 kb on - strand, within ECD_00334at 370.767 kb on - strand, within ECD_00334at 370.881 kb on - strandat 371.012 kb on + strandat 371.155 kb on + strand, within ECD_00335at 371.257 kb on + strand, within ECD_00335at 371.454 kb on + strand, within ECD_00335at 371.473 kb on + strand, within ECD_00335at 371.474 kb on - strand, within ECD_00335at 371.569 kb on + strandat 371.570 kb on - strandat 371.592 kb on + strandat 371.592 kb on + strandat 371.593 kb on - strandat 371.637 kb on + strand, within ECD_00336at 371.637 kb on + strand, within ECD_00336at 371.637 kb on + strand, within ECD_00336at 371.637 kb on + strand, within ECD_00336at 371.637 kb on + strand, within ECD_00336at 371.637 kb on + strand, within ECD_00336at 371.637 kb on + strand, within ECD_00336at 371.638 kb on - strand, within ECD_00336at 371.639 kb on + strand, within ECD_00336at 371.639 kb on + strand, within ECD_00336at 371.639 kb on + strand, within ECD_00336at 371.640 kb on - strand, within ECD_00336at 371.641 kb on + strand, within ECD_00336at 371.641 kb on + strand, within ECD_00336at 371.642 kb on - strand, within ECD_00336at 371.642 kb on - strand, within ECD_00336at 371.642 kb on - strand, within ECD_00336at 371.708 kb on + strand, within ECD_00336

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas29
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369,396 + -0.8
369,589 - ECD_00333 0.16 -0.8
369,589 - ECD_00333 0.16 -0.5
369,589 - ECD_00333 0.16 -0.4
369,589 - ECD_00333 0.16 -1.5
369,647 - ECD_00333 0.23 -1.7
369,729 + ECD_00333 0.33 -1.6
369,729 + ECD_00333 0.33 +0.2
369,729 + ECD_00333 0.33 -2.6
369,730 - ECD_00333 0.33 -2.7
369,730 - ECD_00333 0.33 -2.5
369,737 + ECD_00333 0.34 -0.6
369,793 + ECD_00333 0.41 -1.4
369,823 - ECD_00333 0.45 -0.3
370,006 - ECD_00333 0.67 -0.8
370,006 - ECD_00333 0.67 -1.6
370,166 + ECD_00333 0.87 -0.9
370,167 - ECD_00333 0.87 -1.4
370,167 - ECD_00333 0.87 -2.0
370,249 - +0.6
370,322 + +2.3
370,322 + +0.1
370,332 + -0.9
370,389 - -0.5
370,461 - ECD_00334 0.16 +0.2
370,483 + ECD_00334 0.20 -0.3
370,613 + ECD_00334 0.49 +1.0
370,615 + ECD_00334 0.49 +0.1
370,677 + ECD_00334 0.63 +0.4
370,722 - ECD_00334 0.73 +1.0
370,732 + ECD_00334 0.75 -0.1
370,733 - ECD_00334 0.75 +0.5
370,733 - ECD_00334 0.75 -0.1
370,767 - ECD_00334 0.82 -0.6
370,881 - -0.7
371,012 + -0.4
371,155 + ECD_00335 0.24 +1.4
371,257 + ECD_00335 0.43 -0.6
371,454 + ECD_00335 0.81 -0.3
371,473 + ECD_00335 0.84 +0.5
371,474 - ECD_00335 0.85 +1.5
371,569 + -0.1
371,570 - +0.0
371,592 + -0.5
371,592 + +0.0
371,593 - +0.2
371,637 + ECD_00336 0.17 -0.4
371,637 + ECD_00336 0.17 -0.5
371,637 + ECD_00336 0.17 +0.3
371,637 + ECD_00336 0.17 -0.6
371,637 + ECD_00336 0.17 -1.6
371,637 + ECD_00336 0.17 +0.3
371,637 + ECD_00336 0.17 -0.5
371,638 - ECD_00336 0.18 -0.9
371,639 + ECD_00336 0.18 -0.3
371,639 + ECD_00336 0.18 +0.2
371,639 + ECD_00336 0.18 -0.7
371,640 - ECD_00336 0.19 -1.2
371,641 + ECD_00336 0.19 +0.9
371,641 + ECD_00336 0.19 -0.1
371,642 - ECD_00336 0.20 -0.3
371,642 - ECD_00336 0.20 -0.6
371,642 - ECD_00336 0.20 -0.2
371,708 + ECD_00336 0.54 +1.1

Or see this region's nucleotide sequence