Strain Fitness in Escherichia coli BL21 around ECD_03868

Experiment: Bas06

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03866 and ECD_03867 overlap by 4 nucleotidesECD_03867 and ECD_03868 are separated by 1 nucleotidesECD_03868 and ECD_03869 overlap by 17 nucleotidesECD_03869 and ECD_03870 overlap by 8 nucleotides ECD_03866: ECD_03866 - tyrosine lyase, involved in thiamine-thiazole moiety synthesis, at 4,098,786 to 4,099,919 _03866 ECD_03867: ECD_03867 - thiamine biosynthesis ThiGH complex subunit, at 4,099,916 to 4,100,686 _03867 ECD_03868: ECD_03868 - immediate sulfur donor in thiazole formation, at 4,100,688 to 4,100,888 _03868 ECD_03869: ECD_03869 - adenylyltransferase, modifies ThiS C-terminus, at 4,100,872 to 4,101,627 _03869 ECD_03870: ECD_03870 - thiamine phosphate synthase (thiamine phosphate pyrophosphorylase), at 4,101,620 to 4,102,255 _03870 Position (kb) 4100 4101Strain fitness (log2 ratio) -2 -1 0 1at 4099.693 kb on + strand, within ECD_03866at 4099.700 kb on + strand, within ECD_03866at 4099.701 kb on - strand, within ECD_03866at 4099.837 kb on - strandat 4099.837 kb on - strandat 4100.302 kb on - strand, within ECD_03867at 4100.327 kb on + strand, within ECD_03867at 4100.328 kb on - strand, within ECD_03867at 4100.678 kb on - strandat 4100.678 kb on - strandat 4100.682 kb on - strandat 4100.741 kb on + strand, within ECD_03868at 4100.741 kb on + strand, within ECD_03868at 4100.742 kb on - strand, within ECD_03868at 4100.826 kb on + strand, within ECD_03868at 4100.992 kb on + strand, within ECD_03869at 4100.993 kb on - strand, within ECD_03869at 4100.993 kb on - strand, within ECD_03869at 4101.001 kb on + strand, within ECD_03869at 4101.005 kb on - strand, within ECD_03869at 4101.005 kb on - strand, within ECD_03869at 4101.005 kb on - strand, within ECD_03869at 4101.009 kb on + strand, within ECD_03869at 4101.010 kb on - strand, within ECD_03869at 4101.099 kb on + strand, within ECD_03869at 4101.189 kb on + strand, within ECD_03869at 4101.223 kb on - strand, within ECD_03869at 4101.319 kb on + strand, within ECD_03869at 4101.319 kb on + strand, within ECD_03869at 4101.319 kb on + strand, within ECD_03869at 4101.319 kb on + strand, within ECD_03869at 4101.319 kb on + strand, within ECD_03869at 4101.319 kb on + strand, within ECD_03869at 4101.320 kb on - strand, within ECD_03869at 4101.320 kb on - strand, within ECD_03869at 4101.320 kb on - strand, within ECD_03869at 4101.320 kb on - strand, within ECD_03869at 4101.320 kb on - strand, within ECD_03869at 4101.491 kb on + strand, within ECD_03869at 4101.507 kb on + strand, within ECD_03869at 4101.600 kb on + strandat 4101.600 kb on + strandat 4101.610 kb on - strandat 4101.709 kb on + strand, within ECD_03870at 4101.881 kb on - strand, within ECD_03870at 4101.881 kb on - strand, within ECD_03870at 4101.881 kb on - strand, within ECD_03870at 4101.882 kb on + strand, within ECD_03870at 4101.882 kb on + strand, within ECD_03870at 4101.882 kb on + strand, within ECD_03870at 4101.882 kb on + strand, within ECD_03870at 4101.883 kb on - strand, within ECD_03870at 4101.883 kb on - strand, within ECD_03870at 4101.883 kb on - strand, within ECD_03870at 4101.883 kb on - strand, within ECD_03870

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas06
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4,099,693 + ECD_03866 0.80 +0.2
4,099,700 + ECD_03866 0.81 +0.2
4,099,701 - ECD_03866 0.81 +0.2
4,099,837 - -1.0
4,099,837 - +0.2
4,100,302 - ECD_03867 0.50 +0.6
4,100,327 + ECD_03867 0.53 -0.7
4,100,328 - ECD_03867 0.53 +0.0
4,100,678 - -0.3
4,100,678 - +0.1
4,100,682 - -2.0
4,100,741 + ECD_03868 0.26 -1.6
4,100,741 + ECD_03868 0.26 -0.1
4,100,742 - ECD_03868 0.27 -0.0
4,100,826 + ECD_03868 0.69 -0.5
4,100,992 + ECD_03869 0.16 -0.2
4,100,993 - ECD_03869 0.16 +0.1
4,100,993 - ECD_03869 0.16 +0.0
4,101,001 + ECD_03869 0.17 +0.1
4,101,005 - ECD_03869 0.18 -0.1
4,101,005 - ECD_03869 0.18 +0.5
4,101,005 - ECD_03869 0.18 -0.3
4,101,009 + ECD_03869 0.18 -0.0
4,101,010 - ECD_03869 0.18 -1.1
4,101,099 + ECD_03869 0.30 -0.8
4,101,189 + ECD_03869 0.42 -0.4
4,101,223 - ECD_03869 0.46 -0.3
4,101,319 + ECD_03869 0.59 -0.5
4,101,319 + ECD_03869 0.59 -0.5
4,101,319 + ECD_03869 0.59 +0.2
4,101,319 + ECD_03869 0.59 +0.9
4,101,319 + ECD_03869 0.59 +0.0
4,101,319 + ECD_03869 0.59 +0.2
4,101,320 - ECD_03869 0.59 +0.9
4,101,320 - ECD_03869 0.59 +0.1
4,101,320 - ECD_03869 0.59 +0.0
4,101,320 - ECD_03869 0.59 +0.4
4,101,320 - ECD_03869 0.59 +0.5
4,101,491 + ECD_03869 0.82 -0.2
4,101,507 + ECD_03869 0.84 -0.5
4,101,600 + +0.6
4,101,600 + +0.2
4,101,610 - -0.5
4,101,709 + ECD_03870 0.14 +0.6
4,101,881 - ECD_03870 0.41 -0.1
4,101,881 - ECD_03870 0.41 -0.3
4,101,881 - ECD_03870 0.41 +0.1
4,101,882 + ECD_03870 0.41 +0.3
4,101,882 + ECD_03870 0.41 +0.1
4,101,882 + ECD_03870 0.41 -0.3
4,101,882 + ECD_03870 0.41 +0.6
4,101,883 - ECD_03870 0.41 -0.1
4,101,883 - ECD_03870 0.41 -0.7
4,101,883 - ECD_03870 0.41 -0.3
4,101,883 - ECD_03870 0.41 -0.5

Or see this region's nucleotide sequence