Strain Fitness in Escherichia coli BL21 around ECD_04235

Experiment: Bas06

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_04235 and ECD_04236 are separated by 137 nucleotides ECD_04235: ECD_04235 - L-galactonate oxidoreductase; L-gulonate oxidoreductase, at 4,514,365 to 4,515,387 _04235 ECD_04236: ECD_04236 - OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane-bound) and II (soluble), at 4,515,525 to 4,517,816 _04236 Position (kb) 4514 4515 4516Strain fitness (log2 ratio) -1 0 1at 4513.441 kb on + strandat 4513.853 kb on + strandat 4513.987 kb on - strandat 4513.989 kb on + strandat 4514.033 kb on + strandat 4514.033 kb on + strandat 4514.034 kb on - strandat 4514.034 kb on - strandat 4514.114 kb on + strandat 4514.629 kb on + strand, within ECD_04235at 4514.671 kb on - strand, within ECD_04235at 4514.731 kb on + strand, within ECD_04235at 4514.987 kb on + strand, within ECD_04235at 4515.367 kb on - strandat 4515.402 kb on + strandat 4515.403 kb on - strandat 4515.509 kb on - strandat 4515.527 kb on - strandat 4515.541 kb on - strandat 4515.727 kb on - strandat 4515.763 kb on + strand, within ECD_04236at 4515.959 kb on + strand, within ECD_04236at 4516.016 kb on + strand, within ECD_04236at 4516.016 kb on + strand, within ECD_04236at 4516.022 kb on + strand, within ECD_04236at 4516.226 kb on - strand, within ECD_04236

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas06
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4,513,441 + -1.2
4,513,853 + +0.4
4,513,987 - +0.3
4,513,989 + +1.4
4,514,033 + -0.2
4,514,033 + +0.1
4,514,034 - -0.4
4,514,034 - +0.4
4,514,114 + +0.9
4,514,629 + ECD_04235 0.26 +0.1
4,514,671 - ECD_04235 0.30 -0.1
4,514,731 + ECD_04235 0.36 +0.6
4,514,987 + ECD_04235 0.61 +0.8
4,515,367 - +1.0
4,515,402 + +0.9
4,515,403 - -0.2
4,515,509 - -0.6
4,515,527 - +0.2
4,515,541 - +0.2
4,515,727 - -0.2
4,515,763 + ECD_04236 0.10 -1.1
4,515,959 + ECD_04236 0.19 -0.4
4,516,016 + ECD_04236 0.21 -0.5
4,516,016 + ECD_04236 0.21 -1.3
4,516,022 + ECD_04236 0.22 -0.0
4,516,226 - ECD_04236 0.31 +0.5

Or see this region's nucleotide sequence