Strain Fitness in Escherichia coli BL21 around ECD_00985

Experiment: Bas06

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00984 and ECD_00985 are separated by 19 nucleotidesECD_00985 and ECD_00986 overlap by 13 nucleotides ECD_00984: ECD_00984 - tyrosine-protein kinase, role in O-antigen capsule formation, at 1,047,119 to 1,049,299 _00984 ECD_00985: ECD_00985 - O-antigen capsule forming protein-tyrosine-phosphatase; Etk-P dephosphorylase, at 1,049,319 to 1,049,765 _00985 ECD_00986: ECD_00986 - putative O-antigen capsule outer membrane auxillary protein export channel, at 1,049,753 to 1,050,892 _00986 Position (kb) 1049 1050Strain fitness (log2 ratio) -2 -1 0 1at 1048.574 kb on - strand, within ECD_00984at 1048.724 kb on - strand, within ECD_00984at 1048.778 kb on - strand, within ECD_00984at 1048.812 kb on + strand, within ECD_00984at 1048.812 kb on + strand, within ECD_00984at 1049.122 kb on + strandat 1049.308 kb on - strandat 1049.495 kb on + strand, within ECD_00985at 1049.665 kb on - strand, within ECD_00985at 1050.373 kb on - strand, within ECD_00986at 1050.504 kb on + strand, within ECD_00986at 1050.646 kb on - strand, within ECD_00986at 1050.646 kb on - strand, within ECD_00986at 1050.763 kb on - strand, within ECD_00986

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas06
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1,048,574 - ECD_00984 0.67 +1.1
1,048,724 - ECD_00984 0.74 +0.9
1,048,778 - ECD_00984 0.76 -0.9
1,048,812 + ECD_00984 0.78 -2.1
1,048,812 + ECD_00984 0.78 +0.7
1,049,122 + +0.2
1,049,308 - -0.7
1,049,495 + ECD_00985 0.39 +0.7
1,049,665 - ECD_00985 0.77 -1.0
1,050,373 - ECD_00986 0.54 +1.0
1,050,504 + ECD_00986 0.66 -0.0
1,050,646 - ECD_00986 0.78 -0.4
1,050,646 - ECD_00986 0.78 +0.9
1,050,763 - ECD_00986 0.89 -0.4

Or see this region's nucleotide sequence