Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS08305 and ABIE41_RS08310 are separated by 134 nucleotides ABIE41_RS08310 and ABIE41_RS08315 are separated by 108 nucleotides
ABIE41_RS08305: ABIE41_RS08305 - homospermidine synthase, at 1,714,505 to 1,715,941
_RS08305
ABIE41_RS08310: ABIE41_RS08310 - phosphodiesterase, at 1,716,076 to 1,716,903
_RS08310
ABIE41_RS08315: ABIE41_RS08315 - ABC transporter substrate-binding protein, at 1,717,012 to 1,718,343
_RS08315
Position (kb)
1716
1717 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1715.133 kb on + strand, within ABIE41_RS08305 at 1715.133 kb on + strand, within ABIE41_RS08305 at 1715.134 kb on - strand, within ABIE41_RS08305 at 1715.134 kb on - strand, within ABIE41_RS08305 at 1715.535 kb on + strand, within ABIE41_RS08305 at 1715.536 kb on - strand, within ABIE41_RS08305 at 1715.575 kb on - strand, within ABIE41_RS08305 at 1715.575 kb on - strand, within ABIE41_RS08305 at 1715.730 kb on + strand, within ABIE41_RS08305 at 1715.730 kb on + strand, within ABIE41_RS08305 at 1715.730 kb on + strand, within ABIE41_RS08305 at 1715.730 kb on + strand, within ABIE41_RS08305 at 1715.731 kb on - strand, within ABIE41_RS08305 at 1715.731 kb on - strand, within ABIE41_RS08305 at 1715.922 kb on + strand at 1716.008 kb on + strand at 1716.026 kb on + strand at 1716.027 kb on - strand at 1716.077 kb on + strand at 1716.078 kb on - strand at 1716.092 kb on + strand at 1716.191 kb on + strand, within ABIE41_RS08310 at 1716.191 kb on + strand, within ABIE41_RS08310 at 1716.192 kb on - strand, within ABIE41_RS08310 at 1716.725 kb on + strand, within ABIE41_RS08310 at 1716.725 kb on + strand, within ABIE41_RS08310 at 1716.725 kb on + strand, within ABIE41_RS08310 at 1716.725 kb on + strand, within ABIE41_RS08310 at 1716.725 kb on + strand, within ABIE41_RS08310 at 1716.725 kb on + strand, within ABIE41_RS08310 at 1716.725 kb on + strand, within ABIE41_RS08310 at 1716.726 kb on - strand, within ABIE41_RS08310 at 1716.726 kb on - strand, within ABIE41_RS08310 at 1716.726 kb on - strand, within ABIE41_RS08310 at 1716.726 kb on - strand, within ABIE41_RS08310 at 1716.726 kb on - strand, within ABIE41_RS08310 at 1716.726 kb on - strand, within ABIE41_RS08310 at 1716.847 kb on + strand at 1716.848 kb on + strand at 1717.049 kb on + strand at 1717.049 kb on + strand at 1717.049 kb on + strand at 1717.049 kb on + strand at 1717.049 kb on + strand at 1717.049 kb on + strand at 1717.049 kb on + strand at 1717.049 kb on + strand at 1717.050 kb on - strand at 1717.050 kb on - strand at 1717.050 kb on - strand at 1717.172 kb on + strand, within ABIE41_RS08315 at 1717.172 kb on + strand, within ABIE41_RS08315 at 1717.173 kb on - strand, within ABIE41_RS08315 at 1717.268 kb on + strand, within ABIE41_RS08315 at 1717.269 kb on - strand, within ABIE41_RS08315 at 1717.461 kb on - strand, within ABIE41_RS08315 at 1717.646 kb on + strand, within ABIE41_RS08315 at 1717.646 kb on + strand, within ABIE41_RS08315 at 1717.646 kb on + strand, within ABIE41_RS08315 at 1717.647 kb on - strand, within ABIE41_RS08315 at 1717.647 kb on - strand, within ABIE41_RS08315 at 1717.665 kb on - strand, within ABIE41_RS08315 at 1717.727 kb on + strand, within ABIE41_RS08315 at 1717.727 kb on + strand, within ABIE41_RS08315 at 1717.727 kb on + strand, within ABIE41_RS08315 at 1717.727 kb on + strand, within ABIE41_RS08315 at 1717.727 kb on + strand, within ABIE41_RS08315 at 1717.728 kb on - strand, within ABIE41_RS08315 at 1717.728 kb on - strand, within ABIE41_RS08315 at 1717.733 kb on + strand, within ABIE41_RS08315 at 1717.733 kb on + strand, within ABIE41_RS08315 at 1717.733 kb on + strand, within ABIE41_RS08315 at 1717.734 kb on - strand, within ABIE41_RS08315 at 1717.734 kb on - strand, within ABIE41_RS08315 at 1717.880 kb on + strand, within ABIE41_RS08315 at 1717.880 kb on + strand, within ABIE41_RS08315 at 1717.880 kb on + strand, within ABIE41_RS08315 at 1717.881 kb on - strand, within ABIE41_RS08315 at 1717.881 kb on - strand, within ABIE41_RS08315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.3133 mM remove 1,715,133 + ABIE41_RS08305 0.44 -1.7 1,715,133 + ABIE41_RS08305 0.44 -2.3 1,715,134 - ABIE41_RS08305 0.44 -1.2 1,715,134 - ABIE41_RS08305 0.44 -0.8 1,715,535 + ABIE41_RS08305 0.72 +1.1 1,715,536 - ABIE41_RS08305 0.72 -1.8 1,715,575 - ABIE41_RS08305 0.74 -1.8 1,715,575 - ABIE41_RS08305 0.74 -3.1 1,715,730 + ABIE41_RS08305 0.85 -2.4 1,715,730 + ABIE41_RS08305 0.85 -0.9 1,715,730 + ABIE41_RS08305 0.85 -1.5 1,715,730 + ABIE41_RS08305 0.85 -1.4 1,715,731 - ABIE41_RS08305 0.85 -0.5 1,715,731 - ABIE41_RS08305 0.85 +0.6 1,715,922 + -0.8 1,716,008 + -0.8 1,716,026 + +0.3 1,716,027 - +0.1 1,716,077 + -0.2 1,716,078 - -1.2 1,716,092 + -1.2 1,716,191 + ABIE41_RS08310 0.14 -0.0 1,716,191 + ABIE41_RS08310 0.14 +0.2 1,716,192 - ABIE41_RS08310 0.14 -0.4 1,716,725 + ABIE41_RS08310 0.78 +0.0 1,716,725 + ABIE41_RS08310 0.78 -0.5 1,716,725 + ABIE41_RS08310 0.78 +0.3 1,716,725 + ABIE41_RS08310 0.78 +1.4 1,716,725 + ABIE41_RS08310 0.78 +1.0 1,716,725 + ABIE41_RS08310 0.78 -0.4 1,716,725 + ABIE41_RS08310 0.78 -1.2 1,716,726 - ABIE41_RS08310 0.79 -0.3 1,716,726 - ABIE41_RS08310 0.79 +0.1 1,716,726 - ABIE41_RS08310 0.79 +0.0 1,716,726 - ABIE41_RS08310 0.79 +0.9 1,716,726 - ABIE41_RS08310 0.79 +0.5 1,716,726 - ABIE41_RS08310 0.79 +0.4 1,716,847 + +0.1 1,716,848 + -0.1 1,717,049 + +0.8 1,717,049 + +0.6 1,717,049 + +0.4 1,717,049 + +1.1 1,717,049 + +0.0 1,717,049 + +0.5 1,717,049 + -0.5 1,717,049 + +0.0 1,717,050 - +0.2 1,717,050 - +2.8 1,717,050 - +0.2 1,717,172 + ABIE41_RS08315 0.12 +0.2 1,717,172 + ABIE41_RS08315 0.12 +1.6 1,717,173 - ABIE41_RS08315 0.12 +0.1 1,717,268 + ABIE41_RS08315 0.19 -0.4 1,717,269 - ABIE41_RS08315 0.19 -0.4 1,717,461 - ABIE41_RS08315 0.34 +1.5 1,717,646 + ABIE41_RS08315 0.48 -0.7 1,717,646 + ABIE41_RS08315 0.48 -1.5 1,717,646 + ABIE41_RS08315 0.48 +1.6 1,717,647 - ABIE41_RS08315 0.48 -0.4 1,717,647 - ABIE41_RS08315 0.48 -0.6 1,717,665 - ABIE41_RS08315 0.49 +1.0 1,717,727 + ABIE41_RS08315 0.54 -0.1 1,717,727 + ABIE41_RS08315 0.54 -0.2 1,717,727 + ABIE41_RS08315 0.54 -0.0 1,717,727 + ABIE41_RS08315 0.54 +0.4 1,717,727 + ABIE41_RS08315 0.54 +0.0 1,717,728 - ABIE41_RS08315 0.54 +1.3 1,717,728 - ABIE41_RS08315 0.54 -0.3 1,717,733 + ABIE41_RS08315 0.54 +1.1 1,717,733 + ABIE41_RS08315 0.54 +0.1 1,717,733 + ABIE41_RS08315 0.54 -0.3 1,717,734 - ABIE41_RS08315 0.54 +0.2 1,717,734 - ABIE41_RS08315 0.54 +1.5 1,717,880 + ABIE41_RS08315 0.65 +0.3 1,717,880 + ABIE41_RS08315 0.65 -1.2 1,717,880 + ABIE41_RS08315 0.65 +0.4 1,717,881 - ABIE41_RS08315 0.65 +0.4 1,717,881 - ABIE41_RS08315 0.65 -1.1
Or see this region's nucleotide sequence