Strain Fitness in Escherichia coli BL21 around ECD_00303

Experiment: Bas28

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00301 and ECD_00302 are separated by 1 nucleotidesECD_00302 and ECD_00303 are separated by 17 nucleotidesECD_00303 and ECD_00304 are separated by 9 nucleotidesECD_00304 and ECD_00305 overlap by 4 nucleotides ECD_00301: ECD_00301 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 338,146 to 339,810 _00301 ECD_00302: ECD_00302 - 2,3-dihydroxyphenylpropionate 1,2-dioxygenase, at 339,812 to 340,756 _00302 ECD_00303: ECD_00303 - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase, at 340,774 to 341,640 _00303 ECD_00304: ECD_00304 - 2-keto-4-pentenoate hydratase, at 341,650 to 342,459 _00304 ECD_00305: ECD_00305 - acetaldehyde-CoA dehydrogenase II, NAD-binding, at 342,456 to 343,406 _00305 Position (kb) 340 341 342Strain fitness (log2 ratio) -1 0 1at 339.822 kb on - strandat 339.858 kb on - strandat 339.879 kb on + strandat 339.971 kb on + strand, within ECD_00302at 339.971 kb on + strand, within ECD_00302at 339.987 kb on - strand, within ECD_00302at 340.141 kb on + strand, within ECD_00302at 340.141 kb on + strand, within ECD_00302at 340.141 kb on + strand, within ECD_00302at 340.142 kb on - strand, within ECD_00302at 340.142 kb on - strand, within ECD_00302at 340.462 kb on + strand, within ECD_00302at 340.824 kb on - strandat 341.025 kb on + strand, within ECD_00303at 341.127 kb on + strand, within ECD_00303at 341.235 kb on + strand, within ECD_00303at 341.238 kb on + strand, within ECD_00303at 341.239 kb on - strand, within ECD_00303at 341.410 kb on - strand, within ECD_00303at 341.554 kb on - strandat 341.661 kb on + strandat 341.661 kb on + strandat 341.661 kb on + strandat 341.661 kb on + strandat 341.662 kb on - strandat 341.780 kb on + strand, within ECD_00304at 341.780 kb on + strand, within ECD_00304at 341.781 kb on - strand, within ECD_00304at 341.781 kb on - strand, within ECD_00304at 341.781 kb on - strand, within ECD_00304at 341.791 kb on + strand, within ECD_00304at 341.827 kb on - strand, within ECD_00304at 341.894 kb on + strand, within ECD_00304at 341.895 kb on - strand, within ECD_00304at 342.028 kb on + strand, within ECD_00304at 342.029 kb on - strand, within ECD_00304at 342.048 kb on - strand, within ECD_00304at 342.139 kb on + strand, within ECD_00304at 342.139 kb on + strand, within ECD_00304at 342.140 kb on - strand, within ECD_00304at 342.140 kb on - strand, within ECD_00304at 342.140 kb on - strand, within ECD_00304at 342.211 kb on - strand, within ECD_00304at 342.264 kb on + strand, within ECD_00304at 342.328 kb on - strand, within ECD_00304at 342.347 kb on + strand, within ECD_00304at 342.404 kb on + strandat 342.404 kb on + strandat 342.404 kb on + strandat 342.404 kb on + strandat 342.405 kb on - strandat 342.603 kb on - strand, within ECD_00305

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas28
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339,822 - -0.1
339,858 - +0.3
339,879 + -0.7
339,971 + ECD_00302 0.17 -0.4
339,971 + ECD_00302 0.17 -1.5
339,987 - ECD_00302 0.19 -0.2
340,141 + ECD_00302 0.35 -1.1
340,141 + ECD_00302 0.35 -0.2
340,141 + ECD_00302 0.35 -0.0
340,142 - ECD_00302 0.35 +0.1
340,142 - ECD_00302 0.35 +0.0
340,462 + ECD_00302 0.69 -0.5
340,824 - -0.0
341,025 + ECD_00303 0.29 -0.2
341,127 + ECD_00303 0.41 -1.6
341,235 + ECD_00303 0.53 +0.4
341,238 + ECD_00303 0.54 +0.9
341,239 - ECD_00303 0.54 +1.0
341,410 - ECD_00303 0.73 -0.2
341,554 - -0.2
341,661 + -1.1
341,661 + +0.6
341,661 + +0.3
341,661 + +0.2
341,662 - -0.0
341,780 + ECD_00304 0.16 +0.3
341,780 + ECD_00304 0.16 +0.3
341,781 - ECD_00304 0.16 +0.7
341,781 - ECD_00304 0.16 +0.5
341,781 - ECD_00304 0.16 -0.7
341,791 + ECD_00304 0.17 -0.3
341,827 - ECD_00304 0.22 +0.6
341,894 + ECD_00304 0.30 -0.2
341,895 - ECD_00304 0.30 +1.2
342,028 + ECD_00304 0.47 +0.1
342,029 - ECD_00304 0.47 +0.1
342,048 - ECD_00304 0.49 -0.5
342,139 + ECD_00304 0.60 +0.6
342,139 + ECD_00304 0.60 +0.1
342,140 - ECD_00304 0.60 -0.1
342,140 - ECD_00304 0.60 +0.3
342,140 - ECD_00304 0.60 +0.0
342,211 - ECD_00304 0.69 +1.1
342,264 + ECD_00304 0.76 -0.2
342,328 - ECD_00304 0.84 -1.1
342,347 + ECD_00304 0.86 -0.1
342,404 + -0.6
342,404 + -0.4
342,404 + -0.1
342,404 + -0.1
342,405 - -1.1
342,603 - ECD_00305 0.15 -0.2

Or see this region's nucleotide sequence