Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00002 and ECD_00003 are separated by 1 nucleotides ECD_00003 and ECD_00004 are separated by 0 nucleotides ECD_00004 and ECD_00005 are separated by 212 nucleotides ECD_00005 and ECD_00006 are separated by 152 nucleotides
ECD_00002: ECD_00002 - Bifunctional aspartokinase/homoserine dehydrogenase 1, at 336 to 2,798
_00002
ECD_00003: ECD_00003 - homoserine kinase, at 2,800 to 3,732
_00003
ECD_00004: ECD_00004 - L-threonine synthase, at 3,733 to 5,019
_00004
ECD_00005: ECD_00005 - DUF2502 family putative periplasmic protein, at 5,232 to 5,528
_00005
ECD_00006: ECD_00006 - peroxide resistance protein, lowers intracellular iron, at 5,681 to 6,457
_00006
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2.767 kb on + strand at 2.767 kb on + strand at 2.768 kb on - strand at 2.768 kb on - strand at 2.771 kb on + strand at 2.788 kb on + strand at 2.789 kb on - strand at 2.805 kb on + strand at 2.903 kb on + strand, within ECD_00003 at 2.903 kb on + strand, within ECD_00003 at 2.903 kb on + strand, within ECD_00003 at 2.904 kb on - strand, within ECD_00003 at 3.154 kb on - strand, within ECD_00003 at 3.218 kb on - strand, within ECD_00003 at 3.249 kb on + strand, within ECD_00003 at 3.250 kb on - strand, within ECD_00003 at 3.339 kb on + strand, within ECD_00003 at 3.443 kb on - strand, within ECD_00003 at 3.443 kb on - strand, within ECD_00003 at 3.651 kb on + strand at 3.659 kb on - strand at 3.659 kb on - strand at 3.659 kb on - strand at 3.659 kb on - strand at 3.687 kb on + strand at 3.687 kb on + strand at 3.688 kb on - strand at 3.719 kb on + strand at 3.730 kb on + strand at 3.743 kb on - strand at 3.743 kb on - strand at 4.245 kb on + strand, within ECD_00004 at 4.245 kb on + strand, within ECD_00004 at 4.245 kb on + strand, within ECD_00004 at 4.246 kb on - strand, within ECD_00004 at 4.246 kb on - strand, within ECD_00004 at 4.409 kb on - strand, within ECD_00004 at 4.409 kb on - strand, within ECD_00004 at 4.775 kb on - strand, within ECD_00004 at 4.805 kb on - strand, within ECD_00004 at 4.840 kb on + strand, within ECD_00004 at 4.950 kb on - strand at 4.965 kb on + strand at 4.966 kb on - strand at 4.996 kb on - strand at 5.025 kb on - strand at 5.217 kb on + strand at 5.218 kb on - strand at 5.226 kb on + strand at 5.231 kb on + strand at 5.250 kb on - strand at 5.309 kb on + strand, within ECD_00005 at 5.351 kb on + strand, within ECD_00005 at 5.412 kb on + strand, within ECD_00005 at 5.412 kb on + strand, within ECD_00005 at 5.412 kb on + strand, within ECD_00005 at 5.412 kb on + strand, within ECD_00005 at 5.471 kb on + strand, within ECD_00005 at 5.472 kb on - strand, within ECD_00005 at 5.615 kb on - strand at 5.803 kb on + strand, within ECD_00006 at 5.804 kb on - strand, within ECD_00006 at 5.804 kb on - strand, within ECD_00006 at 5.834 kb on - strand, within ECD_00006 at 5.839 kb on - strand, within ECD_00006 at 5.855 kb on - strand, within ECD_00006 at 5.954 kb on - strand, within ECD_00006 at 6.010 kb on + strand, within ECD_00006 at 6.011 kb on - strand, within ECD_00006 at 6.011 kb on - strand, within ECD_00006 at 6.011 kb on - strand, within ECD_00006
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,767 + -0.2 2,767 + -0.0 2,768 - -0.9 2,768 - +0.1 2,771 + +0.2 2,788 + +0.1 2,789 - -0.1 2,805 + -0.3 2,903 + ECD_00003 0.11 +0.4 2,903 + ECD_00003 0.11 -0.4 2,903 + ECD_00003 0.11 +0.4 2,904 - ECD_00003 0.11 -0.0 3,154 - ECD_00003 0.38 +0.8 3,218 - ECD_00003 0.45 -0.1 3,249 + ECD_00003 0.48 +0.5 3,250 - ECD_00003 0.48 -0.2 3,339 + ECD_00003 0.58 -0.7 3,443 - ECD_00003 0.69 +2.0 3,443 - ECD_00003 0.69 +0.9 3,651 + -0.2 3,659 - +0.3 3,659 - -0.1 3,659 - -0.7 3,659 - -0.0 3,687 + +0.1 3,687 + -0.1 3,688 - +0.9 3,719 + -0.4 3,730 + -1.4 3,743 - +0.1 3,743 - -0.4 4,245 + ECD_00004 0.40 +0.9 4,245 + ECD_00004 0.40 -0.1 4,245 + ECD_00004 0.40 -0.1 4,246 - ECD_00004 0.40 +0.9 4,246 - ECD_00004 0.40 +0.7 4,409 - ECD_00004 0.53 +0.3 4,409 - ECD_00004 0.53 +1.0 4,775 - ECD_00004 0.81 +0.5 4,805 - ECD_00004 0.83 -1.0 4,840 + ECD_00004 0.86 +0.6 4,950 - -0.7 4,965 + -0.2 4,966 - +1.7 4,996 - -0.4 5,025 - +0.2 5,217 + -0.0 5,218 - -0.5 5,226 + -0.8 5,231 + +0.0 5,250 - -0.1 5,309 + ECD_00005 0.26 -0.4 5,351 + ECD_00005 0.40 -0.2 5,412 + ECD_00005 0.61 -0.9 5,412 + ECD_00005 0.61 +0.2 5,412 + ECD_00005 0.61 +0.2 5,412 + ECD_00005 0.61 -0.1 5,471 + ECD_00005 0.80 +0.2 5,472 - ECD_00005 0.81 -0.4 5,615 - +0.3 5,803 + ECD_00006 0.16 -0.3 5,804 - ECD_00006 0.16 -1.0 5,804 - ECD_00006 0.16 -0.8 5,834 - ECD_00006 0.20 -0.5 5,839 - ECD_00006 0.20 -1.1 5,855 - ECD_00006 0.22 -2.6 5,954 - ECD_00006 0.35 -1.4 6,010 + ECD_00006 0.42 -1.5 6,011 - ECD_00006 0.42 -1.7 6,011 - ECD_00006 0.42 -1.1 6,011 - ECD_00006 0.42 -2.0
Or see this region's nucleotide sequence