Strain Fitness in Escherichia coli BL21 around ECD_02331

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02329 and ECD_02330 overlap by 83 nucleotidesECD_02330 and ECD_02331 are separated by 85 nucleotidesECD_02331 and ECD_02332 are separated by 95 nucleotidesECD_02332 and ECD_02333 are separated by 206 nucleotides ECD_02329: ECD_02329 - N-acetylmuramic acid permease, EIIBC component, PTS system, at 2,419,076 to 2,420,500 _02329 ECD_02330: ECD_02330 - penicillin binding protein PBP4B; weak DD-carboxypeptidase activity, at 2,420,418 to 2,420,594 _02330 ECD_02331: ECD_02331 - porphyrinogen oxidase, cytoplasmic, at 2,420,680 to 2,421,579 _02331 ECD_02332: ECD_02332 - RpoE-regulated lipoprotein, at 2,421,675 to 2,422,250 _02332 ECD_02333: ECD_02333 - inner membrane protein, at 2,422,457 to 2,422,906 _02333 Position (kb) 2420 2421 2422Strain fitness (log2 ratio) -2 -1 0 1 2at 2419.684 kb on + strand, within ECD_02329at 2419.759 kb on + strand, within ECD_02329at 2419.759 kb on + strand, within ECD_02329at 2419.760 kb on - strand, within ECD_02329at 2419.889 kb on + strand, within ECD_02329at 2419.900 kb on + strand, within ECD_02329at 2419.901 kb on - strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.930 kb on + strand, within ECD_02329at 2419.931 kb on - strand, within ECD_02329at 2419.931 kb on - strand, within ECD_02329at 2419.946 kb on - strand, within ECD_02329at 2419.961 kb on - strand, within ECD_02329at 2420.079 kb on - strand, within ECD_02329at 2420.080 kb on - strand, within ECD_02329at 2420.135 kb on + strand, within ECD_02329at 2420.257 kb on + strand, within ECD_02329at 2420.334 kb on + strand, within ECD_02329at 2420.455 kb on + strandat 2420.502 kb on - strand, within ECD_02330at 2420.523 kb on + strand, within ECD_02330at 2420.543 kb on + strand, within ECD_02330at 2420.543 kb on + strand, within ECD_02330at 2420.543 kb on + strand, within ECD_02330at 2420.588 kb on + strandat 2420.588 kb on + strandat 2420.588 kb on + strandat 2420.589 kb on - strandat 2420.589 kb on - strandat 2420.600 kb on + strandat 2420.600 kb on + strandat 2420.601 kb on - strandat 2420.602 kb on + strandat 2420.602 kb on + strandat 2420.602 kb on + strandat 2420.603 kb on - strandat 2420.623 kb on + strandat 2420.717 kb on + strandat 2420.766 kb on + strandat 2420.841 kb on - strand, within ECD_02331at 2420.841 kb on - strand, within ECD_02331at 2421.103 kb on - strand, within ECD_02331at 2421.331 kb on - strand, within ECD_02331at 2421.477 kb on + strand, within ECD_02331at 2421.735 kb on + strand, within ECD_02332at 2421.736 kb on - strand, within ECD_02332at 2421.776 kb on + strand, within ECD_02332at 2421.777 kb on - strand, within ECD_02332at 2421.777 kb on - strand, within ECD_02332at 2421.779 kb on + strand, within ECD_02332at 2421.780 kb on - strand, within ECD_02332at 2422.179 kb on - strand, within ECD_02332at 2422.179 kb on - strand, within ECD_02332at 2422.228 kb on - strandat 2422.228 kb on - strandat 2422.274 kb on - strandat 2422.382 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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2,419,684 + ECD_02329 0.43 +1.2
2,419,759 + ECD_02329 0.48 -0.2
2,419,759 + ECD_02329 0.48 -0.2
2,419,760 - ECD_02329 0.48 +0.6
2,419,889 + ECD_02329 0.57 -0.1
2,419,900 + ECD_02329 0.58 -0.5
2,419,901 - ECD_02329 0.58 -0.3
2,419,930 + ECD_02329 0.60 +0.1
2,419,930 + ECD_02329 0.60 -1.1
2,419,930 + ECD_02329 0.60 +0.4
2,419,930 + ECD_02329 0.60 +1.7
2,419,930 + ECD_02329 0.60 -0.7
2,419,931 - ECD_02329 0.60 -1.1
2,419,931 - ECD_02329 0.60 -0.2
2,419,946 - ECD_02329 0.61 -0.9
2,419,961 - ECD_02329 0.62 +1.3
2,420,079 - ECD_02329 0.70 -0.8
2,420,080 - ECD_02329 0.70 +0.5
2,420,135 + ECD_02329 0.74 -2.6
2,420,257 + ECD_02329 0.83 +0.5
2,420,334 + ECD_02329 0.88 +0.9
2,420,455 + -0.8
2,420,502 - ECD_02330 0.47 -1.0
2,420,523 + ECD_02330 0.59 -0.7
2,420,543 + ECD_02330 0.71 -0.2
2,420,543 + ECD_02330 0.71 -0.3
2,420,543 + ECD_02330 0.71 +1.1
2,420,588 + +0.5
2,420,588 + -0.0
2,420,588 + +0.1
2,420,589 - -0.2
2,420,589 - +1.1
2,420,600 + -0.5
2,420,600 + +0.1
2,420,601 - -0.3
2,420,602 + +2.1
2,420,602 + -0.0
2,420,602 + -0.8
2,420,603 - +0.2
2,420,623 + +0.3
2,420,717 + -0.9
2,420,766 + -0.3
2,420,841 - ECD_02331 0.18 -1.6
2,420,841 - ECD_02331 0.18 +0.3
2,421,103 - ECD_02331 0.47 -0.4
2,421,331 - ECD_02331 0.72 -2.0
2,421,477 + ECD_02331 0.89 -1.0
2,421,735 + ECD_02332 0.10 -1.5
2,421,736 - ECD_02332 0.11 +0.1
2,421,776 + ECD_02332 0.18 +0.3
2,421,777 - ECD_02332 0.18 +0.3
2,421,777 - ECD_02332 0.18 +0.4
2,421,779 + ECD_02332 0.18 +0.6
2,421,780 - ECD_02332 0.18 -0.0
2,422,179 - ECD_02332 0.88 +0.5
2,422,179 - ECD_02332 0.88 -0.3
2,422,228 - +0.4
2,422,228 - -1.9
2,422,274 - +0.4
2,422,382 - -0.0

Or see this region's nucleotide sequence