Strain Fitness in Escherichia coli BL21 around ECD_01434

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01432 and ECD_01433 are separated by 12 nucleotidesECD_01433 and ECD_01434 overlap by 8 nucleotidesECD_01434 and ECD_01435 are separated by 194 nucleotidesECD_01435 and ECD_01436 are separated by 145 nucleotides ECD_01432: ECD_01432 - formate dehydrogenase-N, alpha subunit, nitrate-inducible, at 1,503,081 to 1,506,128 _01432 ECD_01433: ECD_01433 - formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible, at 1,506,141 to 1,507,025 _01433 ECD_01434: ECD_01434 - formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible, at 1,507,018 to 1,507,671 _01434 ECD_01435: ECD_01435 - putative DNA-binding transcriptional regulator, at 1,507,866 to 1,508,150 _01435 ECD_01436: ECD_01436 - ethanol-active dehydrogenase/acetaldehyde-active reductase, at 1,508,296 to 1,509,306 _01436 Position (kb) 1507 1508Strain fitness (log2 ratio) -1 0 1at 1506.612 kb on + strand, within ECD_01433at 1507.342 kb on + strand, within ECD_01434at 1507.528 kb on + strand, within ECD_01434at 1507.529 kb on - strand, within ECD_01434at 1507.989 kb on - strand, within ECD_01435at 1508.091 kb on - strand, within ECD_01435at 1508.593 kb on - strand, within ECD_01436

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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1,506,612 + ECD_01433 0.53 +0.6
1,507,342 + ECD_01434 0.50 +0.7
1,507,528 + ECD_01434 0.78 -1.2
1,507,529 - ECD_01434 0.78 +0.1
1,507,989 - ECD_01435 0.43 -0.5
1,508,091 - ECD_01435 0.79 -0.5
1,508,593 - ECD_01436 0.29 -1.1

Or see this region's nucleotide sequence