Strain Fitness in Escherichia coli BL21 around ECD_00006

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00004 and ECD_00005 are separated by 212 nucleotidesECD_00005 and ECD_00006 are separated by 152 nucleotidesECD_00006 and ECD_00007 are separated by 69 nucleotides ECD_00004: ECD_00004 - L-threonine synthase, at 3,733 to 5,019 _00004 ECD_00005: ECD_00005 - DUF2502 family putative periplasmic protein, at 5,232 to 5,528 _00005 ECD_00006: ECD_00006 - peroxide resistance protein, lowers intracellular iron, at 5,681 to 6,457 _00006 ECD_00007: ECD_00007 - putative transporter, at 6,527 to 7,957 _00007 Position (kb) 5 6 7Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4.775 kb on - strand, within ECD_00004at 4.805 kb on - strand, within ECD_00004at 4.840 kb on + strand, within ECD_00004at 4.950 kb on - strandat 4.965 kb on + strandat 4.966 kb on - strandat 4.996 kb on - strandat 5.025 kb on - strandat 5.217 kb on + strandat 5.218 kb on - strandat 5.226 kb on + strandat 5.231 kb on + strandat 5.250 kb on - strandat 5.309 kb on + strand, within ECD_00005at 5.351 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.412 kb on + strand, within ECD_00005at 5.471 kb on + strand, within ECD_00005at 5.472 kb on - strand, within ECD_00005at 5.615 kb on - strandat 5.803 kb on + strand, within ECD_00006at 5.804 kb on - strand, within ECD_00006at 5.804 kb on - strand, within ECD_00006at 5.834 kb on - strand, within ECD_00006at 5.839 kb on - strand, within ECD_00006at 5.855 kb on - strand, within ECD_00006at 5.954 kb on - strand, within ECD_00006at 6.010 kb on + strand, within ECD_00006at 6.011 kb on - strand, within ECD_00006at 6.011 kb on - strand, within ECD_00006at 6.011 kb on - strand, within ECD_00006at 6.028 kb on - strand, within ECD_00006at 6.090 kb on - strand, within ECD_00006at 6.107 kb on + strand, within ECD_00006at 6.115 kb on - strand, within ECD_00006at 6.196 kb on - strand, within ECD_00006at 6.374 kb on + strand, within ECD_00006at 6.375 kb on - strand, within ECD_00006at 6.391 kb on + strandat 6.392 kb on - strandat 6.392 kb on - strandat 6.394 kb on - strandat 6.446 kb on - strandat 6.529 kb on - strandat 6.632 kb on + strandat 6.632 kb on + strandat 6.633 kb on - strandat 6.636 kb on + strandat 6.653 kb on - strandat 6.695 kb on - strand, within ECD_00007at 6.718 kb on + strand, within ECD_00007at 6.718 kb on + strand, within ECD_00007at 6.749 kb on + strand, within ECD_00007at 6.749 kb on + strand, within ECD_00007at 6.749 kb on + strand, within ECD_00007at 6.749 kb on + strand, within ECD_00007at 6.750 kb on - strand, within ECD_00007at 6.750 kb on - strand, within ECD_00007at 6.750 kb on - strand, within ECD_00007at 6.750 kb on - strand, within ECD_00007at 6.750 kb on - strand, within ECD_00007at 6.808 kb on + strand, within ECD_00007at 6.849 kb on + strand, within ECD_00007at 6.952 kb on + strand, within ECD_00007at 6.961 kb on + strand, within ECD_00007at 6.961 kb on + strand, within ECD_00007at 6.961 kb on + strand, within ECD_00007at 6.961 kb on + strand, within ECD_00007at 6.978 kb on + strand, within ECD_00007at 6.978 kb on + strand, within ECD_00007at 6.978 kb on + strand, within ECD_00007at 6.978 kb on + strand, within ECD_00007at 6.979 kb on - strand, within ECD_00007at 6.979 kb on - strand, within ECD_00007at 6.979 kb on - strand, within ECD_00007at 7.001 kb on + strand, within ECD_00007at 7.025 kb on - strand, within ECD_00007at 7.156 kb on + strand, within ECD_00007at 7.157 kb on - strand, within ECD_00007at 7.190 kb on + strand, within ECD_00007at 7.190 kb on + strand, within ECD_00007at 7.195 kb on + strand, within ECD_00007at 7.196 kb on - strand, within ECD_00007at 7.196 kb on - strand, within ECD_00007at 7.196 kb on - strand, within ECD_00007at 7.197 kb on + strand, within ECD_00007at 7.287 kb on - strand, within ECD_00007at 7.412 kb on - strand, within ECD_00007at 7.412 kb on - strand, within ECD_00007

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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4,775 - ECD_00004 0.81 -1.5
4,805 - ECD_00004 0.83 -0.2
4,840 + ECD_00004 0.86 +2.5
4,950 - +0.1
4,965 + +0.2
4,966 - +0.7
4,996 - +0.2
5,025 - -0.4
5,217 + +0.7
5,218 - +0.7
5,226 + -0.1
5,231 + +1.2
5,250 - -0.3
5,309 + ECD_00005 0.26 +1.5
5,351 + ECD_00005 0.40 +0.4
5,412 + ECD_00005 0.61 +0.6
5,412 + ECD_00005 0.61 +1.7
5,412 + ECD_00005 0.61 -0.6
5,412 + ECD_00005 0.61 +1.5
5,471 + ECD_00005 0.80 +0.5
5,472 - ECD_00005 0.81 +0.3
5,615 - +0.4
5,803 + ECD_00006 0.16 +0.9
5,804 - ECD_00006 0.16 -0.6
5,804 - ECD_00006 0.16 -1.8
5,834 - ECD_00006 0.20 -0.5
5,839 - ECD_00006 0.20 -2.1
5,855 - ECD_00006 0.22 -3.0
5,954 - ECD_00006 0.35 -2.3
6,010 + ECD_00006 0.42 -1.1
6,011 - ECD_00006 0.42 -2.2
6,011 - ECD_00006 0.42 -1.9
6,011 - ECD_00006 0.42 -0.8
6,028 - ECD_00006 0.45 -1.1
6,090 - ECD_00006 0.53 -2.0
6,107 + ECD_00006 0.55 -2.0
6,115 - ECD_00006 0.56 -1.9
6,196 - ECD_00006 0.66 +0.1
6,374 + ECD_00006 0.89 -1.1
6,375 - ECD_00006 0.89 -1.6
6,391 + -0.8
6,392 - -0.8
6,392 - +0.0
6,394 - -1.4
6,446 - -0.4
6,529 - +0.5
6,632 + -0.2
6,632 + -0.3
6,633 - +0.5
6,636 + +3.9
6,653 - -1.9
6,695 - ECD_00007 0.12 +0.5
6,718 + ECD_00007 0.13 +0.3
6,718 + ECD_00007 0.13 +2.5
6,749 + ECD_00007 0.16 -0.1
6,749 + ECD_00007 0.16 -0.6
6,749 + ECD_00007 0.16 -0.1
6,749 + ECD_00007 0.16 -0.3
6,750 - ECD_00007 0.16 -0.0
6,750 - ECD_00007 0.16 -0.9
6,750 - ECD_00007 0.16 -0.6
6,750 - ECD_00007 0.16 +0.2
6,750 - ECD_00007 0.16 -0.4
6,808 + ECD_00007 0.20 -0.5
6,849 + ECD_00007 0.23 +1.2
6,952 + ECD_00007 0.30 -1.0
6,961 + ECD_00007 0.30 -0.9
6,961 + ECD_00007 0.30 -0.7
6,961 + ECD_00007 0.30 -0.9
6,961 + ECD_00007 0.30 +1.1
6,978 + ECD_00007 0.32 -0.2
6,978 + ECD_00007 0.32 -0.6
6,978 + ECD_00007 0.32 -1.3
6,978 + ECD_00007 0.32 -0.8
6,979 - ECD_00007 0.32 -0.2
6,979 - ECD_00007 0.32 -0.6
6,979 - ECD_00007 0.32 -0.6
7,001 + ECD_00007 0.33 +0.7
7,025 - ECD_00007 0.35 +0.2
7,156 + ECD_00007 0.44 +0.5
7,157 - ECD_00007 0.44 -0.3
7,190 + ECD_00007 0.46 +0.5
7,190 + ECD_00007 0.46 -0.3
7,195 + ECD_00007 0.47 -0.5
7,196 - ECD_00007 0.47 -0.4
7,196 - ECD_00007 0.47 -0.1
7,196 - ECD_00007 0.47 -0.5
7,197 + ECD_00007 0.47 +1.1
7,287 - ECD_00007 0.53 -1.1
7,412 - ECD_00007 0.62 -0.0
7,412 - ECD_00007 0.62 -0.8

Or see this region's nucleotide sequence