Strain Fitness in Escherichia coli BL21 around ECD_04289

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02413 and ECD_04290 are separated by 83 nucleotidesECD_04290 and ECD_04289 overlap by 4 nucleotidesECD_04289 and ECD_04342 are separated by 360 nucleotidesECD_04342 and ECD_02414 are separated by 344 nucleotides ECD_02413: ECD_02413 - 3-mercaptopyruvate sulfurtransferase, at 2,516,974 to 2,517,819 _02413 ECD_04290: ECD_04290 - transposase, at 2,517,903 to 2,518,424 _04290 ECD_04289: ECD_04289 - transposase, at 2,518,421 to 2,519,260 _04289 ECD_04342: ECD_04342 - hypothetical protein, at 2,519,621 to 2,519,737 _04342 ECD_02414: ECD_02414 - rhodanase-like enzyme, sulfur transfer from thiosulfate, at 2,520,082 to 2,520,858 _02414 Position (kb) 2518 2519 2520Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2517.452 kb on + strand, within ECD_02413at 2517.452 kb on + strand, within ECD_02413at 2517.452 kb on + strand, within ECD_02413at 2517.545 kb on + strand, within ECD_02413at 2517.661 kb on + strand, within ECD_02413at 2519.354 kb on - strandat 2519.522 kb on - strandat 2519.672 kb on - strand, within ECD_04342at 2519.672 kb on - strand, within ECD_04342at 2519.672 kb on - strand, within ECD_04342at 2519.680 kb on - strand, within ECD_04342at 2519.680 kb on - strand, within ECD_04342at 2519.683 kb on - strand, within ECD_04342at 2519.819 kb on - strandat 2519.988 kb on + strandat 2519.989 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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2,517,452 + ECD_02413 0.57 +0.2
2,517,452 + ECD_02413 0.57 +1.4
2,517,452 + ECD_02413 0.57 +0.7
2,517,545 + ECD_02413 0.67 -0.4
2,517,661 + ECD_02413 0.81 +0.8
2,519,354 - +0.3
2,519,522 - -0.2
2,519,672 - ECD_04342 0.44 -0.9
2,519,672 - ECD_04342 0.44 -0.0
2,519,672 - ECD_04342 0.44 +0.4
2,519,680 - ECD_04342 0.50 -0.0
2,519,680 - ECD_04342 0.50 +3.4
2,519,683 - ECD_04342 0.53 -0.7
2,519,819 - -2.1
2,519,988 + +2.5
2,519,989 - +0.7

Or see this region's nucleotide sequence