Experiment: Bas52
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_02413 and ECD_04290 are separated by 83 nucleotides ECD_04290 and ECD_04289 overlap by 4 nucleotides ECD_04289 and ECD_04342 are separated by 360 nucleotides ECD_04342 and ECD_02414 are separated by 344 nucleotides
ECD_02413: ECD_02413 - 3-mercaptopyruvate sulfurtransferase, at 2,516,974 to 2,517,819
_02413
ECD_04290: ECD_04290 - transposase, at 2,517,903 to 2,518,424
_04290
ECD_04289: ECD_04289 - transposase, at 2,518,421 to 2,519,260
_04289
ECD_04342: ECD_04342 - hypothetical protein, at 2,519,621 to 2,519,737
_04342
ECD_02414: ECD_02414 - rhodanase-like enzyme, sulfur transfer from thiosulfate, at 2,520,082 to 2,520,858
_02414
Position (kb)
2518
2519
2520 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2517.452 kb on + strand, within ECD_02413 at 2517.452 kb on + strand, within ECD_02413 at 2517.452 kb on + strand, within ECD_02413 at 2517.545 kb on + strand, within ECD_02413 at 2517.661 kb on + strand, within ECD_02413 at 2519.354 kb on - strand at 2519.522 kb on - strand at 2519.672 kb on - strand, within ECD_04342 at 2519.672 kb on - strand, within ECD_04342 at 2519.672 kb on - strand, within ECD_04342 at 2519.680 kb on - strand, within ECD_04342 at 2519.680 kb on - strand, within ECD_04342 at 2519.683 kb on - strand, within ECD_04342 at 2519.819 kb on - strand at 2519.988 kb on + strand at 2519.989 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas52 remove 2,517,452 + ECD_02413 0.57 +0.2 2,517,452 + ECD_02413 0.57 +1.4 2,517,452 + ECD_02413 0.57 +0.7 2,517,545 + ECD_02413 0.67 -0.4 2,517,661 + ECD_02413 0.81 +0.8 2,519,354 - +0.3 2,519,522 - -0.2 2,519,672 - ECD_04342 0.44 -0.9 2,519,672 - ECD_04342 0.44 -0.0 2,519,672 - ECD_04342 0.44 +0.4 2,519,680 - ECD_04342 0.50 -0.0 2,519,680 - ECD_04342 0.50 +3.4 2,519,683 - ECD_04342 0.53 -0.7 2,519,819 - -2.1 2,519,988 + +2.5 2,519,989 - +0.7
Or see this region's nucleotide sequence