Experiment: Bas52
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_03064 and ECD_03065 overlap by 32 nucleotides ECD_03065 and ECD_03066 are separated by 6 nucleotides ECD_03066 and ECD_03067 are separated by 47 nucleotides
ECD_03064: ECD_03064 - periplasmic membrane-anchored LPS-binding protein; LPS export protein, at 3,211,336 to 3,211,911
_03064
ECD_03065: ECD_03065 - lipopolysaccharide export ABC transporter periplasmic binding protein; Lipid A binding protein; LPS export and assembly protein, at 3,211,880 to 3,212,437
_03065
ECD_03066: ECD_03066 - lipopolysaccharide export ABC transporter ATPase, at 3,212,444 to 3,213,169
_03066
ECD_03067: ECD_03067 - RNA polymerase, sigma 54 (sigma N) factor, at 3,213,217 to 3,214,650
_03067
Position (kb) 3212
3213
3214 Strain fitness (log2 ratio) -1
0
1
2 at 3211.764 kb on + strand, within ECD_03064 at 3211.764 kb on + strand, within ECD_03064 at 3211.787 kb on + strand, within ECD_03064 at 3211.789 kb on + strand, within ECD_03064 at 3213.193 kb on + strand at 3214.123 kb on + strand, within ECD_03067 at 3214.123 kb on + strand, within ECD_03067
Per-strain Table
Position Strand Gene LocusTag Fraction Bas52 remove 3,211,764 + ECD_03064 0.74 +1.3 3,211,764 + ECD_03064 0.74 +1.4 3,211,787 + ECD_03064 0.78 -1.1 3,211,789 + ECD_03064 0.79 +1.3 3,213,193 + +0.4 3,214,123 + ECD_03067 0.63 +1.1 3,214,123 + ECD_03067 0.63 +1.8
Or see this region's nucleotide sequence