Strain Fitness in Escherichia coli BL21 around ECD_01017

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01015 and ECD_01016 are separated by 230 nucleotidesECD_01016 and ECD_01017 are separated by 39 nucleotidesECD_01017 and ECD_01018 are separated by 422 nucleotides ECD_01015: ECD_01015 - pyrimidine oxygenase, FMN-dependent, at 1,077,145 to 1,078,293 _01015 ECD_01016: ECD_01016 - rut operon transcriptional repressor for, at 1,078,524 to 1,079,162 _01016 ECD_01017: ECD_01017 - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, at 1,079,202 to 1,083,164 _01017 ECD_01018: ECD_01018 - proline:sodium symporter, at 1,083,587 to 1,085,095 _01018 Position (kb) 1079 1080 1081 1082 1083 1084Strain fitness (log2 ratio) -2 -1 0 1 2at 1079.182 kb on - strandat 1079.273 kb on - strandat 1079.448 kb on - strandat 1079.482 kb on - strandat 1079.482 kb on - strandat 1079.685 kb on - strand, within ECD_01017at 1079.685 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.934 kb on - strand, within ECD_01017at 1079.937 kb on - strand, within ECD_01017at 1079.937 kb on - strand, within ECD_01017at 1079.937 kb on - strand, within ECD_01017at 1079.948 kb on - strand, within ECD_01017at 1079.948 kb on - strand, within ECD_01017at 1079.948 kb on - strand, within ECD_01017at 1079.988 kb on - strand, within ECD_01017at 1080.457 kb on - strand, within ECD_01017at 1080.457 kb on - strand, within ECD_01017at 1080.459 kb on + strand, within ECD_01017at 1080.460 kb on - strand, within ECD_01017at 1080.462 kb on + strand, within ECD_01017at 1080.463 kb on - strand, within ECD_01017at 1080.463 kb on - strand, within ECD_01017at 1080.815 kb on + strand, within ECD_01017at 1080.843 kb on - strand, within ECD_01017at 1080.987 kb on - strand, within ECD_01017at 1081.114 kb on - strand, within ECD_01017at 1081.151 kb on + strand, within ECD_01017at 1081.152 kb on - strand, within ECD_01017at 1081.510 kb on + strand, within ECD_01017at 1081.551 kb on - strand, within ECD_01017at 1081.729 kb on - strand, within ECD_01017at 1081.784 kb on - strand, within ECD_01017at 1081.799 kb on - strand, within ECD_01017at 1082.203 kb on + strand, within ECD_01017at 1082.204 kb on - strand, within ECD_01017at 1082.204 kb on - strand, within ECD_01017at 1082.266 kb on + strand, within ECD_01017at 1082.266 kb on + strand, within ECD_01017at 1082.267 kb on - strand, within ECD_01017at 1082.267 kb on - strand, within ECD_01017at 1082.315 kb on - strand, within ECD_01017at 1082.589 kb on - strand, within ECD_01017at 1082.643 kb on - strand, within ECD_01017at 1082.713 kb on - strand, within ECD_01017at 1083.065 kb on + strandat 1083.171 kb on - strandat 1083.171 kb on - strandat 1083.539 kb on + strandat 1083.595 kb on + strandat 1083.626 kb on + strandat 1083.642 kb on + strandat 1083.689 kb on + strandat 1083.691 kb on + strandat 1083.691 kb on + strandat 1083.691 kb on + strandat 1084.152 kb on + strand, within ECD_01018

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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1,079,182 - -0.6
1,079,273 - +0.7
1,079,448 - -0.7
1,079,482 - -0.1
1,079,482 - -0.1
1,079,685 - ECD_01017 0.12 -1.1
1,079,685 - ECD_01017 0.12 -0.1
1,079,689 - ECD_01017 0.12 +0.1
1,079,689 - ECD_01017 0.12 +1.3
1,079,689 - ECD_01017 0.12 +0.6
1,079,689 - ECD_01017 0.12 +0.5
1,079,934 - ECD_01017 0.18 +0.1
1,079,937 - ECD_01017 0.19 -0.1
1,079,937 - ECD_01017 0.19 -0.4
1,079,937 - ECD_01017 0.19 +0.1
1,079,948 - ECD_01017 0.19 +0.3
1,079,948 - ECD_01017 0.19 -2.0
1,079,948 - ECD_01017 0.19 +2.6
1,079,988 - ECD_01017 0.20 +0.6
1,080,457 - ECD_01017 0.32 -1.5
1,080,457 - ECD_01017 0.32 +0.2
1,080,459 + ECD_01017 0.32 -0.3
1,080,460 - ECD_01017 0.32 -0.9
1,080,462 + ECD_01017 0.32 +0.0
1,080,463 - ECD_01017 0.32 -0.8
1,080,463 - ECD_01017 0.32 -1.8
1,080,815 + ECD_01017 0.41 -1.3
1,080,843 - ECD_01017 0.41 -0.9
1,080,987 - ECD_01017 0.45 -0.3
1,081,114 - ECD_01017 0.48 -0.2
1,081,151 + ECD_01017 0.49 +0.6
1,081,152 - ECD_01017 0.49 -0.9
1,081,510 + ECD_01017 0.58 +1.2
1,081,551 - ECD_01017 0.59 +0.5
1,081,729 - ECD_01017 0.64 +0.3
1,081,784 - ECD_01017 0.65 +1.1
1,081,799 - ECD_01017 0.66 +0.3
1,082,203 + ECD_01017 0.76 -1.7
1,082,204 - ECD_01017 0.76 +0.3
1,082,204 - ECD_01017 0.76 -0.1
1,082,266 + ECD_01017 0.77 -0.4
1,082,266 + ECD_01017 0.77 -1.7
1,082,267 - ECD_01017 0.77 +0.6
1,082,267 - ECD_01017 0.77 +2.4
1,082,315 - ECD_01017 0.79 +1.1
1,082,589 - ECD_01017 0.85 -0.5
1,082,643 - ECD_01017 0.87 +0.0
1,082,713 - ECD_01017 0.89 -0.2
1,083,065 + -0.4
1,083,171 - -0.2
1,083,171 - +0.8
1,083,539 + +0.1
1,083,595 + +0.4
1,083,626 + +1.9
1,083,642 + +0.7
1,083,689 + +0.8
1,083,691 + +1.6
1,083,691 + +0.7
1,083,691 + +0.6
1,084,152 + ECD_01018 0.37 +0.6

Or see this region's nucleotide sequence