Strain Fitness in Escherichia coli BL21 around ECD_00986

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00984 and ECD_00985 are separated by 19 nucleotidesECD_00985 and ECD_00986 overlap by 13 nucleotidesECD_00986 and ECD_00987 are separated by 45 nucleotides ECD_00984: ECD_00984 - tyrosine-protein kinase, role in O-antigen capsule formation, at 1,047,119 to 1,049,299 _00984 ECD_00985: ECD_00985 - O-antigen capsule forming protein-tyrosine-phosphatase; Etk-P dephosphorylase, at 1,049,319 to 1,049,765 _00985 ECD_00986: ECD_00986 - putative O-antigen capsule outer membrane auxillary protein export channel, at 1,049,753 to 1,050,892 _00986 ECD_00987: ECD_00987 - putative O-antigen capsule production periplasmic protein, at 1,050,938 to 1,053,034 _00987 Position (kb) 1049 1050 1051Strain fitness (log2 ratio) -1 0 1at 1048.778 kb on - strand, within ECD_00984at 1048.812 kb on + strand, within ECD_00984at 1048.812 kb on + strand, within ECD_00984at 1049.122 kb on + strandat 1049.308 kb on - strandat 1049.495 kb on + strand, within ECD_00985at 1049.665 kb on - strand, within ECD_00985at 1050.373 kb on - strand, within ECD_00986at 1050.504 kb on + strand, within ECD_00986at 1050.646 kb on - strand, within ECD_00986at 1050.646 kb on - strand, within ECD_00986at 1050.763 kb on - strand, within ECD_00986at 1050.829 kb on + strandat 1050.829 kb on + strandat 1050.830 kb on - strandat 1051.109 kb on - strandat 1051.139 kb on + strandat 1051.139 kb on + strandat 1051.139 kb on + strandat 1051.359 kb on + strand, within ECD_00987at 1051.360 kb on - strand, within ECD_00987at 1051.360 kb on - strand, within ECD_00987at 1051.455 kb on + strand, within ECD_00987at 1051.456 kb on - strand, within ECD_00987at 1051.597 kb on + strand, within ECD_00987at 1051.617 kb on + strand, within ECD_00987at 1051.755 kb on + strand, within ECD_00987

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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1,048,778 - ECD_00984 0.76 +0.3
1,048,812 + ECD_00984 0.78 -0.4
1,048,812 + ECD_00984 0.78 +1.6
1,049,122 + -0.1
1,049,308 - -1.7
1,049,495 + ECD_00985 0.39 -1.7
1,049,665 - ECD_00985 0.77 +0.6
1,050,373 - ECD_00986 0.54 +1.5
1,050,504 + ECD_00986 0.66 -1.0
1,050,646 - ECD_00986 0.78 +0.3
1,050,646 - ECD_00986 0.78 -0.4
1,050,763 - ECD_00986 0.89 -0.7
1,050,829 + -0.9
1,050,829 + -1.0
1,050,830 - +0.6
1,051,109 - -0.5
1,051,139 + -0.4
1,051,139 + +0.5
1,051,139 + +1.7
1,051,359 + ECD_00987 0.20 -1.4
1,051,360 - ECD_00987 0.20 +1.3
1,051,360 - ECD_00987 0.20 -0.0
1,051,455 + ECD_00987 0.25 +0.7
1,051,456 - ECD_00987 0.25 -0.5
1,051,597 + ECD_00987 0.31 +1.1
1,051,617 + ECD_00987 0.32 -0.7
1,051,755 + ECD_00987 0.39 -0.0

Or see this region's nucleotide sequence