Strain Fitness in Escherichia coli BL21 around ECD_00445

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00443 and ECD_00444 are separated by 60 nucleotidesECD_00444 and ECD_00445 overlap by 11 nucleotidesECD_00445 and ECD_00446 overlap by 33 nucleotidesECD_00446 and ECD_00447 overlap by 4 nucleotides ECD_00443: ECD_00443 - DnaK co-chaperone, thioredoxin-like protein, at 486,572 to 487,426 _00443 ECD_00444: ECD_00444 - putative oxidoreductase, at 487,487 to 488,296 _00444 ECD_00445: ECD_00445 - acyl-CoA thioesterase 1 and protease I and lysophospholipase L1, at 488,286 to 488,912 _00445 ECD_00446: ECD_00446 - putative ABC transporter ATPase, at 488,880 to 489,566 _00446 ECD_00447: ECD_00447 - putative ABC transporter permease, at 489,563 to 491,977 _00447 Position (kb) 488 489Strain fitness (log2 ratio) -1 0 1 2 3at 487.305 kb on - strand, within ECD_00443at 487.499 kb on + strandat 487.545 kb on - strandat 487.598 kb on - strand, within ECD_00444at 487.611 kb on - strand, within ECD_00444at 487.611 kb on - strand, within ECD_00444at 487.643 kb on - strand, within ECD_00444at 487.807 kb on - strand, within ECD_00444at 487.807 kb on - strand, within ECD_00444at 487.876 kb on + strand, within ECD_00444at 487.998 kb on + strand, within ECD_00444at 487.998 kb on + strand, within ECD_00444at 487.999 kb on - strand, within ECD_00444at 487.999 kb on - strand, within ECD_00444at 487.999 kb on - strand, within ECD_00444at 488.021 kb on - strand, within ECD_00444at 488.032 kb on - strand, within ECD_00444at 488.041 kb on + strand, within ECD_00444at 488.237 kb on - strandat 488.340 kb on - strandat 488.401 kb on + strand, within ECD_00445at 488.414 kb on + strand, within ECD_00445at 488.471 kb on + strand, within ECD_00445at 488.498 kb on - strand, within ECD_00445at 488.514 kb on + strand, within ECD_00445at 488.514 kb on + strand, within ECD_00445at 488.515 kb on - strand, within ECD_00445at 488.524 kb on - strand, within ECD_00445at 488.720 kb on + strand, within ECD_00445at 488.720 kb on + strand, within ECD_00445at 488.720 kb on + strand, within ECD_00445at 488.725 kb on - strand, within ECD_00445at 488.747 kb on + strand, within ECD_00445at 488.748 kb on - strand, within ECD_00445at 489.101 kb on - strand, within ECD_00446at 489.104 kb on + strand, within ECD_00446at 489.104 kb on + strand, within ECD_00446at 489.105 kb on - strand, within ECD_00446at 489.105 kb on - strand, within ECD_00446at 489.105 kb on - strand, within ECD_00446at 489.105 kb on - strand, within ECD_00446at 489.105 kb on - strand, within ECD_00446at 489.171 kb on - strand, within ECD_00446at 489.280 kb on + strand, within ECD_00446at 489.428 kb on + strand, within ECD_00446at 489.609 kb on + strandat 489.610 kb on - strandat 489.875 kb on - strand, within ECD_00447at 489.888 kb on - strand, within ECD_00447at 489.888 kb on - strand, within ECD_00447at 489.888 kb on - strand, within ECD_00447at 489.904 kb on + strand, within ECD_00447at 489.905 kb on - strand, within ECD_00447

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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487,305 - ECD_00443 0.86 +0.6
487,499 + -1.0
487,545 - +0.5
487,598 - ECD_00444 0.14 +0.7
487,611 - ECD_00444 0.15 +0.4
487,611 - ECD_00444 0.15 +0.1
487,643 - ECD_00444 0.19 +0.9
487,807 - ECD_00444 0.40 +0.6
487,807 - ECD_00444 0.40 -0.6
487,876 + ECD_00444 0.48 -0.6
487,998 + ECD_00444 0.63 -0.6
487,998 + ECD_00444 0.63 +0.7
487,999 - ECD_00444 0.63 +1.4
487,999 - ECD_00444 0.63 -0.7
487,999 - ECD_00444 0.63 -1.2
488,021 - ECD_00444 0.66 +1.0
488,032 - ECD_00444 0.67 -0.1
488,041 + ECD_00444 0.68 +0.4
488,237 - +0.1
488,340 - +0.2
488,401 + ECD_00445 0.18 +0.0
488,414 + ECD_00445 0.20 +2.3
488,471 + ECD_00445 0.30 +0.0
488,498 - ECD_00445 0.34 +1.1
488,514 + ECD_00445 0.36 -0.0
488,514 + ECD_00445 0.36 +1.4
488,515 - ECD_00445 0.37 +0.8
488,524 - ECD_00445 0.38 +1.1
488,720 + ECD_00445 0.69 +0.0
488,720 + ECD_00445 0.69 +0.0
488,720 + ECD_00445 0.69 +0.4
488,725 - ECD_00445 0.70 +3.2
488,747 + ECD_00445 0.74 +0.3
488,748 - ECD_00445 0.74 -0.3
489,101 - ECD_00446 0.32 -0.9
489,104 + ECD_00446 0.33 +0.7
489,104 + ECD_00446 0.33 -0.8
489,105 - ECD_00446 0.33 +0.6
489,105 - ECD_00446 0.33 +0.4
489,105 - ECD_00446 0.33 -0.2
489,105 - ECD_00446 0.33 +2.4
489,105 - ECD_00446 0.33 -0.8
489,171 - ECD_00446 0.42 -0.2
489,280 + ECD_00446 0.58 -1.4
489,428 + ECD_00446 0.80 -1.6
489,609 + +0.4
489,610 - +1.1
489,875 - ECD_00447 0.13 +0.4
489,888 - ECD_00447 0.13 +0.0
489,888 - ECD_00447 0.13 -0.9
489,888 - ECD_00447 0.13 -0.3
489,904 + ECD_00447 0.14 -0.6
489,905 - ECD_00447 0.14 +0.7

Or see this region's nucleotide sequence