Strain Fitness in Escherichia coli BL21 around ECD_03486

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03484 and ECD_03485 overlap by 82 nucleotidesECD_03485 and ECD_03486 are separated by 570 nucleotidesECD_03486 and ECD_03487 are separated by 15 nucleotidesECD_03487 and ECD_03488 overlap by 8 nucleotides ECD_03484: ECD_03484 - IS1 protein InsB, at 3,669,648 to 3,670,151 _03484 ECD_03485: ECD_03485 - IS1 protein InsA, at 3,670,070 to 3,670,345 _03485 ECD_03486: ECD_03486 - UDP-glucose:(Glucosyl) LPS alpha1,3-glucosyltransferase WaaO, at 3,670,916 to 3,671,932 _03486 ECD_03487: ECD_03487 - kinase that phosphorylates core heptose of lipopolysaccharide, at 3,671,948 to 3,672,745 _03487 ECD_03488: ECD_03488 - UDP-glucose:(heptosyl)lipopolysaccharide alpha-1,3-glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I, at 3,672,738 to 3,673,862 _03488 Position (kb) 3670 3671 3672Strain fitness (log2 ratio) -1 0 1 2at 3670.370 kb on + strandat 3670.435 kb on - strandat 3670.438 kb on - strandat 3670.438 kb on - strandat 3670.438 kb on - strandat 3670.438 kb on - strandat 3670.438 kb on - strandat 3670.438 kb on - strandat 3670.438 kb on - strandat 3670.474 kb on - strandat 3670.562 kb on + strandat 3670.586 kb on + strandat 3671.221 kb on + strand, within ECD_03486at 3671.363 kb on - strand, within ECD_03486at 3671.366 kb on - strand, within ECD_03486at 3671.410 kb on + strand, within ECD_03486at 3671.410 kb on + strand, within ECD_03486at 3671.411 kb on - strand, within ECD_03486at 3671.447 kb on - strand, within ECD_03486at 3671.479 kb on + strand, within ECD_03486at 3671.598 kb on + strand, within ECD_03486at 3671.609 kb on - strand, within ECD_03486at 3671.771 kb on - strand, within ECD_03486at 3671.920 kb on - strandat 3671.933 kb on - strandat 3672.253 kb on + strand, within ECD_03487at 3672.253 kb on + strand, within ECD_03487at 3672.255 kb on + strand, within ECD_03487at 3672.472 kb on + strand, within ECD_03487at 3672.534 kb on - strand, within ECD_03487at 3672.791 kb on - strandat 3672.791 kb on - strandat 3672.793 kb on - strandat 3672.793 kb on - strandat 3672.804 kb on - strandat 3672.804 kb on - strandat 3672.811 kb on + strandat 3672.812 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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3,670,370 + +0.9
3,670,435 - +0.6
3,670,438 - -1.0
3,670,438 - +0.2
3,670,438 - +0.1
3,670,438 - -0.0
3,670,438 - +0.6
3,670,438 - -0.1
3,670,438 - +0.3
3,670,474 - +0.4
3,670,562 + -0.1
3,670,586 + -0.2
3,671,221 + ECD_03486 0.30 -0.4
3,671,363 - ECD_03486 0.44 +0.2
3,671,366 - ECD_03486 0.44 -0.2
3,671,410 + ECD_03486 0.49 -1.2
3,671,410 + ECD_03486 0.49 -0.5
3,671,411 - ECD_03486 0.49 -1.2
3,671,447 - ECD_03486 0.52 -1.1
3,671,479 + ECD_03486 0.55 +0.1
3,671,598 + ECD_03486 0.67 -1.3
3,671,609 - ECD_03486 0.68 -0.3
3,671,771 - ECD_03486 0.84 +0.7
3,671,920 - -0.5
3,671,933 - -0.7
3,672,253 + ECD_03487 0.38 -0.6
3,672,253 + ECD_03487 0.38 -0.3
3,672,255 + ECD_03487 0.38 -0.3
3,672,472 + ECD_03487 0.66 +0.8
3,672,534 - ECD_03487 0.73 +1.3
3,672,791 - -0.2
3,672,791 - +1.2
3,672,793 - -0.4
3,672,793 - +0.3
3,672,804 - -0.2
3,672,804 - +0.3
3,672,811 + +1.8
3,672,812 - -1.2

Or see this region's nucleotide sequence