Strain Fitness in Escherichia coli BL21 around ECD_02365

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02363 and ECD_02364 are separated by 27 nucleotidesECD_02364 and ECD_02365 are separated by 109 nucleotidesECD_02365 and ECD_02366 are separated by 73 nucleotides ECD_02363: ECD_02363 - N-succinyl-diaminopimelate deacylase, at 2,456,096 to 2,457,223 _02363 ECD_02364: ECD_02364 - putative membrane protein, UPF0370 family, at 2,457,251 to 2,457,451 _02364 ECD_02365: ECD_02365 - palmitoyl-CoA esterase activity, uncertain physiological substrate, at 2,457,561 to 2,458,259 _02365 ECD_02366: ECD_02366 - elongator methionine tRNA (ac4C34) acetyltransferase, at 2,458,333 to 2,460,348 _02366 Position (kb) 2457 2458 2459Strain fitness (log2 ratio) -1 0 1at 2457.246 kb on + strandat 2457.246 kb on + strandat 2457.247 kb on - strandat 2457.346 kb on + strand, within ECD_02364at 2457.449 kb on + strandat 2457.538 kb on + strandat 2457.551 kb on - strandat 2457.597 kb on - strandat 2457.607 kb on - strandat 2457.638 kb on + strand, within ECD_02365at 2457.640 kb on + strand, within ECD_02365at 2457.858 kb on - strand, within ECD_02365at 2457.897 kb on - strand, within ECD_02365at 2458.343 kb on - strandat 2458.392 kb on + strandat 2458.392 kb on + strandat 2458.471 kb on - strandat 2458.471 kb on - strandat 2458.474 kb on + strandat 2458.475 kb on - strandat 2458.565 kb on - strand, within ECD_02366at 2458.775 kb on + strand, within ECD_02366at 2458.776 kb on - strand, within ECD_02366at 2458.899 kb on + strand, within ECD_02366at 2458.921 kb on - strand, within ECD_02366at 2459.248 kb on + strand, within ECD_02366

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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2,457,246 + -0.2
2,457,246 + +0.2
2,457,247 - -1.7
2,457,346 + ECD_02364 0.47 -0.2
2,457,449 + -0.0
2,457,538 + -0.1
2,457,551 - -0.4
2,457,597 - -0.2
2,457,607 - -0.8
2,457,638 + ECD_02365 0.11 -0.2
2,457,640 + ECD_02365 0.11 -0.1
2,457,858 - ECD_02365 0.42 -0.6
2,457,897 - ECD_02365 0.48 -0.0
2,458,343 - +0.0
2,458,392 + +1.2
2,458,392 + +0.0
2,458,471 - -0.8
2,458,471 - +0.5
2,458,474 + -0.8
2,458,475 - +0.4
2,458,565 - ECD_02366 0.12 +0.2
2,458,775 + ECD_02366 0.22 +0.2
2,458,776 - ECD_02366 0.22 +0.1
2,458,899 + ECD_02366 0.28 +0.5
2,458,921 - ECD_02366 0.29 +0.6
2,459,248 + ECD_02366 0.45 -1.1

Or see this region's nucleotide sequence