Strain Fitness in Escherichia coli BL21 around ECD_02201

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02199 and ECD_02200 overlap by 4 nucleotidesECD_02200 and ECD_02201 are separated by 67 nucleotidesECD_02201 and ECD_02202 are separated by 6 nucleotides ECD_02199: ECD_02199 - putative ISRSO11-transposase OrfA protein, at 2,275,808 to 2,276,329 _02199 ECD_02200: ECD_02200 - YadA protein, at 2,276,326 to 2,277,165 _02200 ECD_02201: ECD_02201 - NADH:ubiquinone oxidoreductase, membrane subunit N, at 2,277,233 to 2,278,690 _02201 ECD_02202: ECD_02202 - NADH:ubiquinone oxidoreductase, membrane subunit M, at 2,278,697 to 2,280,226 _02202 Position (kb) 2277 2278 2279Strain fitness (log2 ratio) -2 -1 0 1at 2277.168 kb on + strandat 2277.168 kb on + strandat 2277.220 kb on - strandat 2277.234 kb on + strandat 2277.234 kb on + strandat 2277.234 kb on + strandat 2277.235 kb on - strandat 2277.262 kb on + strandat 2277.265 kb on + strandat 2277.266 kb on - strandat 2277.284 kb on + strandat 2277.300 kb on - strandat 2277.417 kb on + strand, within ECD_02201at 2277.417 kb on + strand, within ECD_02201at 2277.424 kb on - strand, within ECD_02201at 2277.502 kb on - strand, within ECD_02201at 2277.784 kb on + strand, within ECD_02201at 2277.784 kb on + strand, within ECD_02201at 2277.784 kb on + strand, within ECD_02201at 2277.785 kb on - strand, within ECD_02201at 2278.000 kb on - strand, within ECD_02201at 2278.001 kb on + strand, within ECD_02201at 2278.002 kb on - strand, within ECD_02201at 2278.002 kb on - strand, within ECD_02201at 2278.002 kb on - strand, within ECD_02201at 2278.002 kb on - strand, within ECD_02201at 2278.102 kb on + strand, within ECD_02201at 2278.159 kb on - strand, within ECD_02201at 2278.159 kb on - strand, within ECD_02201at 2278.159 kb on - strand, within ECD_02201at 2278.362 kb on - strand, within ECD_02201at 2278.374 kb on + strand, within ECD_02201at 2278.375 kb on - strand, within ECD_02201at 2278.398 kb on + strand, within ECD_02201at 2278.399 kb on - strand, within ECD_02201at 2278.451 kb on + strand, within ECD_02201at 2278.502 kb on - strand, within ECD_02201at 2278.577 kb on - strandat 2278.682 kb on + strandat 2278.683 kb on - strandat 2278.683 kb on - strandat 2278.708 kb on + strandat 2278.712 kb on - strandat 2278.712 kb on - strandat 2279.236 kb on - strand, within ECD_02202at 2279.239 kb on + strand, within ECD_02202at 2279.356 kb on + strand, within ECD_02202at 2279.357 kb on - strand, within ECD_02202at 2279.565 kb on + strand, within ECD_02202at 2279.566 kb on - strand, within ECD_02202at 2279.590 kb on + strand, within ECD_02202at 2279.591 kb on - strand, within ECD_02202at 2279.637 kb on + strand, within ECD_02202

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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2,277,168 + -1.5
2,277,168 + -0.6
2,277,220 - -0.5
2,277,234 + +0.1
2,277,234 + -0.0
2,277,234 + -0.2
2,277,235 - +0.1
2,277,262 + +0.1
2,277,265 + +0.7
2,277,266 - -0.4
2,277,284 + +0.5
2,277,300 - -0.9
2,277,417 + ECD_02201 0.13 +0.2
2,277,417 + ECD_02201 0.13 -2.4
2,277,424 - ECD_02201 0.13 -1.2
2,277,502 - ECD_02201 0.18 -1.6
2,277,784 + ECD_02201 0.38 -1.0
2,277,784 + ECD_02201 0.38 -1.0
2,277,784 + ECD_02201 0.38 -1.8
2,277,785 - ECD_02201 0.38 -1.4
2,278,000 - ECD_02201 0.53 -1.0
2,278,001 + ECD_02201 0.53 -0.0
2,278,002 - ECD_02201 0.53 -2.0
2,278,002 - ECD_02201 0.53 -0.5
2,278,002 - ECD_02201 0.53 -0.3
2,278,002 - ECD_02201 0.53 -2.6
2,278,102 + ECD_02201 0.60 -1.1
2,278,159 - ECD_02201 0.64 -0.5
2,278,159 - ECD_02201 0.64 -1.0
2,278,159 - ECD_02201 0.64 -0.7
2,278,362 - ECD_02201 0.77 +0.0
2,278,374 + ECD_02201 0.78 -0.3
2,278,375 - ECD_02201 0.78 -1.8
2,278,398 + ECD_02201 0.80 -0.8
2,278,399 - ECD_02201 0.80 -1.3
2,278,451 + ECD_02201 0.84 -0.6
2,278,502 - ECD_02201 0.87 -2.5
2,278,577 - -0.8
2,278,682 + -0.6
2,278,683 - -0.8
2,278,683 - -0.0
2,278,708 + -1.2
2,278,712 - -0.7
2,278,712 - -2.4
2,279,236 - ECD_02202 0.35 -0.7
2,279,239 + ECD_02202 0.35 -0.4
2,279,356 + ECD_02202 0.43 -1.8
2,279,357 - ECD_02202 0.43 -0.5
2,279,565 + ECD_02202 0.57 -1.5
2,279,566 - ECD_02202 0.57 -0.7
2,279,590 + ECD_02202 0.58 -1.7
2,279,591 - ECD_02202 0.58 +0.1
2,279,637 + ECD_02202 0.61 +0.2

Or see this region's nucleotide sequence