Strain Fitness in Escherichia coli BL21 around ECD_02135

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02133 and ECD_02134 overlap by 4 nucleotidesECD_02134 and ECD_02135 overlap by 11 nucleotidesECD_02135 and ECD_04337 are separated by 108 nucleotidesECD_04337 and ECD_02136 are separated by 134 nucleotides ECD_02133: ECD_02133 - nitrate reductase, periplasmic, large subunit, at 2,193,917 to 2,196,403 _02133 ECD_02134: ECD_02134 - assembly protein for periplasmic nitrate reductase, at 2,196,400 to 2,196,663 _02134 ECD_02135: ECD_02135 - ferredoxin-type protein, role in electron transfer to periplasmic nitrate reductase NapA, at 2,196,653 to 2,197,147 _02135 ECD_04337: ECD_04337 - hypothetical protein, at 2,197,256 to 2,197,420 _04337 ECD_02136: ECD_02136 - ecotin, a serine protease inhibitor, at 2,197,555 to 2,198,043 _02136 Position (kb) 2196 2197 2198Strain fitness (log2 ratio) -1 0 1 2at 2195.659 kb on + strand, within ECD_02133at 2195.675 kb on - strand, within ECD_02133at 2195.828 kb on - strand, within ECD_02133at 2195.836 kb on + strand, within ECD_02133at 2196.082 kb on + strand, within ECD_02133at 2196.129 kb on + strand, within ECD_02133at 2196.370 kb on + strandat 2196.654 kb on + strandat 2196.669 kb on + strandat 2196.670 kb on - strandat 2196.681 kb on - strandat 2196.739 kb on + strand, within ECD_02135at 2196.740 kb on - strand, within ECD_02135at 2196.781 kb on + strand, within ECD_02135at 2196.803 kb on - strand, within ECD_02135at 2197.494 kb on + strandat 2197.494 kb on + strandat 2197.825 kb on + strand, within ECD_02136at 2197.825 kb on + strand, within ECD_02136at 2197.982 kb on + strand, within ECD_02136at 2197.982 kb on + strand, within ECD_02136at 2197.982 kb on + strand, within ECD_02136at 2198.041 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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2,195,659 + ECD_02133 0.70 +0.5
2,195,675 - ECD_02133 0.71 -0.0
2,195,828 - ECD_02133 0.77 -0.5
2,195,836 + ECD_02133 0.77 +0.6
2,196,082 + ECD_02133 0.87 +0.7
2,196,129 + ECD_02133 0.89 -0.0
2,196,370 + -0.5
2,196,654 + +0.6
2,196,669 + +1.8
2,196,670 - -0.7
2,196,681 - -0.1
2,196,739 + ECD_02135 0.17 -0.4
2,196,740 - ECD_02135 0.18 +0.1
2,196,781 + ECD_02135 0.26 -0.7
2,196,803 - ECD_02135 0.30 -1.1
2,197,494 + +0.7
2,197,494 + +0.9
2,197,825 + ECD_02136 0.55 -0.4
2,197,825 + ECD_02136 0.55 +0.5
2,197,982 + ECD_02136 0.87 +1.0
2,197,982 + ECD_02136 0.87 -0.5
2,197,982 + ECD_02136 0.87 -0.1
2,198,041 + +0.3

Or see this region's nucleotide sequence