Strain Fitness in Escherichia coli BL21 around ECD_01425

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01423 and ECD_01424 overlap by 4 nucleotidesECD_01424 and ECD_01425 overlap by 1 nucleotidesECD_01425 and ECD_01426 overlap by 4 nucleotides ECD_01423: ECD_01423 - nitrate reductase 2 (NRZ), gamma subunit, at 1,491,618 to 1,492,298 _01423 ECD_01424: ECD_01424 - nitrate reductase 2 (NRZ), delta subunit (assembly subunit), at 1,492,295 to 1,492,990 _01424 ECD_01425: ECD_01425 - nitrate reductase 2 (NRZ), beta subunit, at 1,492,990 to 1,494,534 _01425 ECD_01426: ECD_01426 - nitrate reductase 2 (NRZ), alpha subunit, at 1,494,531 to 1,498,271 _01426 Position (kb) 1492 1493 1494 1495Strain fitness (log2 ratio) -1 0 1at 1492.670 kb on + strand, within ECD_01424at 1492.674 kb on + strand, within ECD_01424at 1493.047 kb on - strandat 1493.181 kb on - strand, within ECD_01425at 1493.205 kb on - strand, within ECD_01425at 1493.291 kb on + strand, within ECD_01425at 1493.383 kb on - strand, within ECD_01425at 1493.547 kb on - strand, within ECD_01425at 1494.178 kb on - strand, within ECD_01425at 1494.787 kb on + strandat 1494.787 kb on + strandat 1494.971 kb on - strand, within ECD_01426at 1495.138 kb on + strand, within ECD_01426at 1495.208 kb on - strand, within ECD_01426

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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1,492,670 + ECD_01424 0.54 +0.9
1,492,674 + ECD_01424 0.54 -0.1
1,493,047 - +0.1
1,493,181 - ECD_01425 0.12 +0.1
1,493,205 - ECD_01425 0.14 -0.2
1,493,291 + ECD_01425 0.19 -0.7
1,493,383 - ECD_01425 0.25 +0.0
1,493,547 - ECD_01425 0.36 -0.9
1,494,178 - ECD_01425 0.77 -0.3
1,494,787 + -0.1
1,494,787 + -0.2
1,494,971 - ECD_01426 0.12 +0.8
1,495,138 + ECD_01426 0.16 -0.2
1,495,208 - ECD_01426 0.18 +0.6

Or see this region's nucleotide sequence