Strain Fitness in Escherichia coli BL21 around ECD_01221

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01220 and ECD_01221 overlap by 4 nucleotidesECD_01221 and ECD_01222 are separated by 52 nucleotidesECD_01222 and ECD_01223 are separated by 34 nucleotides ECD_01220: ECD_01220 - oligopeptide ABC transporter ATPase, at 1,290,346 to 1,291,359 _01220 ECD_01221: ECD_01221 - oligopeptide ABC transporter ATPase, at 1,291,356 to 1,292,360 _01221 ECD_01222: ECD_01222 - UPF0263 family protein, at 1,292,413 to 1,292,742 _01222 ECD_01223: ECD_01223 - cardiolipin synthase 1, at 1,292,777 to 1,294,237 _01223 Position (kb) 1291 1292 1293Strain fitness (log2 ratio) -1 0 1at 1290.703 kb on + strand, within ECD_01220at 1290.703 kb on + strand, within ECD_01220at 1290.705 kb on + strand, within ECD_01220at 1290.705 kb on + strand, within ECD_01220at 1290.747 kb on + strand, within ECD_01220at 1290.896 kb on + strand, within ECD_01220at 1290.906 kb on + strand, within ECD_01220at 1291.067 kb on + strand, within ECD_01220at 1291.072 kb on + strand, within ECD_01220at 1291.117 kb on + strand, within ECD_01220at 1291.117 kb on + strand, within ECD_01220at 1291.135 kb on + strand, within ECD_01220at 1291.206 kb on + strand, within ECD_01220at 1291.284 kb on + strandat 1291.335 kb on + strandat 1291.746 kb on + strand, within ECD_01221at 1292.100 kb on + strand, within ECD_01221at 1292.218 kb on + strand, within ECD_01221at 1293.189 kb on - strand, within ECD_01223

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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1,290,703 + ECD_01220 0.35 -0.2
1,290,703 + ECD_01220 0.35 +0.3
1,290,705 + ECD_01220 0.35 +0.4
1,290,705 + ECD_01220 0.35 +0.7
1,290,747 + ECD_01220 0.40 -0.2
1,290,896 + ECD_01220 0.54 -0.2
1,290,906 + ECD_01220 0.55 +0.5
1,291,067 + ECD_01220 0.71 +1.0
1,291,072 + ECD_01220 0.72 -0.4
1,291,117 + ECD_01220 0.76 -0.8
1,291,117 + ECD_01220 0.76 +0.3
1,291,135 + ECD_01220 0.78 -0.9
1,291,206 + ECD_01220 0.85 -1.5
1,291,284 + -0.2
1,291,335 + +1.3
1,291,746 + ECD_01221 0.39 -0.1
1,292,100 + ECD_01221 0.74 -0.7
1,292,218 + ECD_01221 0.86 -0.4
1,293,189 - ECD_01223 0.28 -0.6

Or see this region's nucleotide sequence