Strain Fitness in Escherichia coli BL21 around ECD_01188

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01186 and ECD_01187 overlap by 1 nucleotidesECD_01187 and ECD_01188 overlap by 4 nucleotidesECD_01188 and ECD_01189 are separated by 3 nucleotidesECD_01189 and ECD_01190 are separated by 35 nucleotides ECD_01186: ECD_01186 - peptide chain release factor RF-1, at 1,253,160 to 1,254,242 _01186 ECD_01187: ECD_01187 - RF-1 and RF-2 N5-glutamine methyltransferase, at 1,254,242 to 1,255,075 _01187 ECD_01188: ECD_01188 - SIRB family inner membrane protein, at 1,255,072 to 1,255,464 _01188 ECD_01189: ECD_01189 - transglutaminase-like TPR-repeat protein, at 1,255,468 to 1,256,277 _01189 ECD_01190: ECD_01190 - 3-deoxy-D-manno-octulosonate 8-phosphate synthase, at 1,256,313 to 1,257,167 _01190 Position (kb) 1255 1256Strain fitness (log2 ratio) -1 0 1at 1255.232 kb on + strand, within ECD_01188at 1255.341 kb on + strand, within ECD_01188at 1255.631 kb on + strand, within ECD_01189at 1255.631 kb on + strand, within ECD_01189at 1255.631 kb on + strand, within ECD_01189at 1255.917 kb on + strand, within ECD_01189at 1256.244 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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1,255,232 + ECD_01188 0.41 -1.0
1,255,341 + ECD_01188 0.68 -0.2
1,255,631 + ECD_01189 0.20 +0.2
1,255,631 + ECD_01189 0.20 +0.4
1,255,631 + ECD_01189 0.20 +0.3
1,255,917 + ECD_01189 0.55 +0.9
1,256,244 + -0.8

Or see this region's nucleotide sequence