Strain Fitness in Escherichia coli BL21 around ECD_01034

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01032 and ECD_01033 are separated by 101 nucleotidesECD_01033 and ECD_01034 are separated by 63 nucleotidesECD_01034 and ECD_01035 are separated by 26 nucleotidesECD_01035 and ECD_01036 are separated by 24 nucleotidesECD_01036 and ECD_01037 are separated by 4 nucleotides ECD_01032: ECD_01032 - redox enzyme maturation protein (REMP) chaperone for YcdX, at 1,102,207 to 1,102,761 _01032 ECD_01033: ECD_01033 - DUF1097 family inner membrane protein, at 1,102,863 to 1,103,354 _01033 ECD_01034: ECD_01034 - curli production assembly/transport outer membrane lipoprotein, at 1,103,418 to 1,104,251 _01034 ECD_01035: ECD_01035 - curli nucleation outer membrane protein, at 1,104,278 to 1,104,694 _01035 ECD_01036: ECD_01036 - curlin secretion specificity factor, at 1,104,719 to 1,105,108 _01036 ECD_01037: ECD_01037 - csgBAC operon transcriptional regulator, at 1,105,113 to 1,105,763 _01037 Position (kb) 1103 1104 1105Strain fitness (log2 ratio) -1 0 1at 1102.621 kb on + strand, within ECD_01032at 1102.809 kb on + strandat 1102.870 kb on + strandat 1102.870 kb on + strandat 1102.880 kb on + strandat 1103.067 kb on + strand, within ECD_01033at 1103.067 kb on + strand, within ECD_01033at 1103.072 kb on + strand, within ECD_01033at 1103.141 kb on + strand, within ECD_01033at 1103.571 kb on - strand, within ECD_01034at 1103.571 kb on - strand, within ECD_01034

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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1,102,621 + ECD_01032 0.75 +0.4
1,102,809 + +1.7
1,102,870 + -1.3
1,102,870 + +0.4
1,102,880 + +0.3
1,103,067 + ECD_01033 0.41 +0.2
1,103,067 + ECD_01033 0.41 +0.2
1,103,072 + ECD_01033 0.42 -0.9
1,103,141 + ECD_01033 0.57 -0.6
1,103,571 - ECD_01034 0.18 +0.2
1,103,571 - ECD_01034 0.18 -1.2

Or see this region's nucleotide sequence