Strain Fitness in Escherichia coli BL21 around ECD_00053

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00051 and ECD_00052 are separated by 191 nucleotidesECD_00052 and ECD_00053 are separated by 77 nucleotidesECD_00053 and ECD_00054 are separated by 6 nucleotidesECD_00054 and ECD_00055 are separated by 2 nucleotides ECD_00051: ECD_00051 - potassium:proton antiporter, at 51,965 to 53,827 _00051 ECD_00052: ECD_00052 - dihydrofolate reductase, at 54,019 to 54,498 _00052 ECD_00053: ECD_00053 - diadenosine tetraphosphatase, at 54,576 to 55,418 _00053 ECD_00054: ECD_00054 - protein associated with Co2+ and Mg2+ efflux, at 55,425 to 55,802 _00054 ECD_00055: ECD_00055 - 16S rRNA m(6)A1518, m(6)A1519 dimethyltransferase, SAM-dependent, at 55,805 to 56,626 _00055 Position (kb) 54 55 56Strain fitness (log2 ratio) -2 -1 0 1at 53.576 kb on + strand, within ECD_00051at 53.615 kb on - strand, within ECD_00051at 53.700 kb on + strandat 53.701 kb on - strandat 53.701 kb on - strandat 53.740 kb on - strandat 53.825 kb on + strandat 53.840 kb on + strandat 53.979 kb on + strandat 53.983 kb on + strandat 53.983 kb on + strandat 53.983 kb on + strandat 53.985 kb on + strandat 54.561 kb on + strandat 54.562 kb on - strandat 54.562 kb on - strandat 54.605 kb on + strandat 54.606 kb on - strandat 54.632 kb on - strandat 54.700 kb on - strand, within ECD_00053at 54.701 kb on + strand, within ECD_00053at 54.701 kb on + strand, within ECD_00053at 54.702 kb on - strand, within ECD_00053at 54.835 kb on + strand, within ECD_00053at 54.879 kb on - strand, within ECD_00053at 54.952 kb on + strand, within ECD_00053at 54.957 kb on - strand, within ECD_00053at 54.957 kb on - strand, within ECD_00053at 55.242 kb on + strand, within ECD_00053at 55.243 kb on - strand, within ECD_00053at 55.356 kb on - strandat 55.404 kb on - strandat 55.409 kb on - strandat 55.558 kb on + strand, within ECD_00054at 55.558 kb on + strand, within ECD_00054at 55.710 kb on - strand, within ECD_00054at 55.711 kb on + strand, within ECD_00054at 55.724 kb on - strand, within ECD_00054at 55.778 kb on + strandat 55.779 kb on - strandat 55.839 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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53,576 + ECD_00051 0.86 -0.5
53,615 - ECD_00051 0.89 -1.1
53,700 + -0.4
53,701 - -0.5
53,701 - -0.1
53,740 - +0.3
53,825 + +0.4
53,840 + -0.0
53,979 + -1.4
53,983 + -0.6
53,983 + -0.6
53,983 + +0.4
53,985 + -0.6
54,561 + +0.3
54,562 - +0.7
54,562 - +0.4
54,605 + +0.7
54,606 - +0.2
54,632 - +0.8
54,700 - ECD_00053 0.15 -0.9
54,701 + ECD_00053 0.15 -2.1
54,701 + ECD_00053 0.15 +1.4
54,702 - ECD_00053 0.15 -0.4
54,835 + ECD_00053 0.31 -1.2
54,879 - ECD_00053 0.36 -0.5
54,952 + ECD_00053 0.45 -0.4
54,957 - ECD_00053 0.45 +1.3
54,957 - ECD_00053 0.45 +0.7
55,242 + ECD_00053 0.79 +0.2
55,243 - ECD_00053 0.79 +0.1
55,356 - -0.5
55,404 - -0.4
55,409 - -0.6
55,558 + ECD_00054 0.35 -0.5
55,558 + ECD_00054 0.35 -0.3
55,710 - ECD_00054 0.75 -0.8
55,711 + ECD_00054 0.76 -1.0
55,724 - ECD_00054 0.79 +0.1
55,778 + -1.6
55,779 - -0.3
55,839 + -0.1

Or see this region's nucleotide sequence