Strain Fitness in Escherichia coli BL21 around ECD_01614

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01611 and ECD_01612 are separated by 46 nucleotidesECD_01612 and ECD_01613 are separated by 200 nucleotidesECD_01613 and ECD_01614 are separated by 2 nucleotidesECD_01614 and ECD_01615 overlap by 1 nucleotides ECD_01611: ECD_01611 - outer membrane lipoprotein, at 1,665,072 to 1,665,539 _01611 ECD_01612: ECD_01612 - global transcriptional regulator, at 1,665,586 to 1,666,020 _01612 ECD_01613: ECD_01613 - DUF1656 family putative inner membrane efflux pump associated protein, at 1,666,221 to 1,666,457 _01613 ECD_01614: ECD_01614 - putative membrane fusion protein (MFP) of YdhJK efflux pump, at 1,666,460 to 1,667,317 _01614 ECD_01615: ECD_01615 - putative efflux protein (PET) component of YdhJK efflux pump, at 1,667,317 to 1,669,329 _01615 Position (kb) 1666 1667 1668Strain fitness (log2 ratio) -1 0 1at 1665.537 kb on + strandat 1666.340 kb on - strand, within ECD_01613at 1666.621 kb on + strand, within ECD_01614at 1666.622 kb on - strand, within ECD_01614at 1666.636 kb on + strand, within ECD_01614at 1667.096 kb on + strand, within ECD_01614at 1667.885 kb on + strand, within ECD_01615at 1668.062 kb on + strand, within ECD_01615at 1668.063 kb on - strand, within ECD_01615at 1668.309 kb on - strand, within ECD_01615

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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1,665,537 + +0.5
1,666,340 - ECD_01613 0.50 +0.7
1,666,621 + ECD_01614 0.19 +0.3
1,666,622 - ECD_01614 0.19 +0.1
1,666,636 + ECD_01614 0.21 +1.6
1,667,096 + ECD_01614 0.74 +1.0
1,667,885 + ECD_01615 0.28 -0.4
1,668,062 + ECD_01615 0.37 -1.5
1,668,063 - ECD_01615 0.37 -0.8
1,668,309 - ECD_01615 0.49 +0.5

Or see this region's nucleotide sequence