Strain Fitness in Escherichia coli BL21 around ECD_01208

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01205 and ECD_t00025 are separated by 361 nucleotidesECD_t00025 and ECD_t00026 are separated by 209 nucleotidesECD_t00026 and ECD_01208 are separated by 159 nucleotidesECD_01208 and ECD_01209 are separated by 49 nucleotidesECD_01209 and ECD_01210 are separated by 73 nucleotides ECD_01205: ECD_01205 - nitrate reductase 1, gamma (cytochrome b(NR)) subunit, at 1,273,997 to 1,274,674 _01205 ECD_t00025: ECD_t00025 - tRNA-Tyr, at 1,275,036 to 1,275,120 _t00025 ECD_t00026: ECD_t00026 - tRNA-Tyr, at 1,275,330 to 1,275,414 _t00026 ECD_01208: ECD_01208 - formyltetrahydrofolate hydrolase, at 1,275,574 to 1,276,416 _01208 ECD_01209: ECD_01209 - UPF0225 family protein, at 1,276,466 to 1,276,924 _01209 ECD_01210: ECD_01210 - putative patatin-like family phospholipase, at 1,276,998 to 1,277,942 _01210 Position (kb) 1275 1276 1277Strain fitness (log2 ratio) -2 -1 0 1at 1274.644 kb on + strandat 1274.644 kb on + strandat 1274.645 kb on - strandat 1274.645 kb on - strandat 1274.648 kb on + strandat 1275.144 kb on - strandat 1275.174 kb on - strandat 1275.329 kb on - strandat 1275.329 kb on - strandat 1275.329 kb on - strandat 1275.470 kb on - strandat 1275.696 kb on - strand, within ECD_01208at 1275.696 kb on - strand, within ECD_01208at 1275.700 kb on - strand, within ECD_01208at 1275.762 kb on - strand, within ECD_01208at 1275.781 kb on - strand, within ECD_01208at 1276.046 kb on - strand, within ECD_01208at 1276.066 kb on - strand, within ECD_01208at 1276.544 kb on - strand, within ECD_01209at 1276.553 kb on - strand, within ECD_01209at 1276.553 kb on - strand, within ECD_01209at 1276.686 kb on - strand, within ECD_01209at 1276.740 kb on - strand, within ECD_01209at 1276.867 kb on - strand, within ECD_01209at 1276.867 kb on - strand, within ECD_01209at 1276.882 kb on - strandat 1276.885 kb on + strandat 1276.886 kb on - strandat 1276.901 kb on - strandat 1276.975 kb on + strandat 1276.975 kb on + strandat 1276.976 kb on - strandat 1276.976 kb on - strandat 1277.184 kb on + strand, within ECD_01210at 1277.387 kb on + strand, within ECD_01210

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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1,274,644 + +1.1
1,274,644 + -0.1
1,274,645 - -1.7
1,274,645 - +0.8
1,274,648 + -0.2
1,275,144 - +0.0
1,275,174 - +0.3
1,275,329 - +0.1
1,275,329 - -0.0
1,275,329 - +0.9
1,275,470 - -0.3
1,275,696 - ECD_01208 0.14 -0.1
1,275,696 - ECD_01208 0.14 -0.6
1,275,700 - ECD_01208 0.15 -0.9
1,275,762 - ECD_01208 0.22 +0.7
1,275,781 - ECD_01208 0.25 -1.9
1,276,046 - ECD_01208 0.56 +1.7
1,276,066 - ECD_01208 0.58 +1.3
1,276,544 - ECD_01209 0.17 -0.7
1,276,553 - ECD_01209 0.19 -0.3
1,276,553 - ECD_01209 0.19 +0.1
1,276,686 - ECD_01209 0.48 -1.2
1,276,740 - ECD_01209 0.60 +0.1
1,276,867 - ECD_01209 0.87 +1.3
1,276,867 - ECD_01209 0.87 +0.5
1,276,882 - -1.0
1,276,885 + -0.2
1,276,886 - +1.1
1,276,901 - -0.4
1,276,975 + -0.9
1,276,975 + +1.0
1,276,976 - +1.3
1,276,976 - -0.9
1,277,184 + ECD_01210 0.20 -0.4
1,277,387 + ECD_01210 0.41 -0.5

Or see this region's nucleotide sequence