Strain Fitness in Escherichia coli BL21 around ECD_00022

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00021 and ECD_00022 are separated by 432 nucleotidesECD_00022 and ECD_00023 are separated by 433 nucleotidesECD_00023 and ECD_00024 overlap by 82 nucleotides ECD_00021: ECD_00021 - putative usher protein, at 20,630 to 21,775 _00021 ECD_00022: ECD_00022 - hypothetical protein, at 22,208 to 22,912 _00022 ECD_00023: ECD_00023 - IS1 protein InsA, at 23,346 to 23,621 _00023 ECD_00024: ECD_00024 - IS1 protein InsB, at 23,540 to 24,043 _00024 Position (kb) 22 23Strain fitness (log2 ratio) -1 0 1at 21.213 kb on - strand, within ECD_00021at 21.862 kb on + strandat 23.202 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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21,213 - ECD_00021 0.51 +1.3
21,862 + -0.2
23,202 + +0.4

Or see this region's nucleotide sequence