Strain Fitness in Escherichia coli BL21 around ECD_02133

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02131 and ECD_02132 overlap by 14 nucleotidesECD_02132 and ECD_02133 are separated by 6 nucleotidesECD_02133 and ECD_02134 overlap by 4 nucleotidesECD_02134 and ECD_02135 overlap by 11 nucleotidesECD_02135 and ECD_04337 are separated by 108 nucleotides ECD_02131: ECD_02131 - ferredoxin-type protein, at 2,192,365 to 2,193,228 _02131 ECD_02132: ECD_02132 - ferredoxin-type protein, at 2,193,215 to 2,193,910 _02132 ECD_02133: ECD_02133 - nitrate reductase, periplasmic, large subunit, at 2,193,917 to 2,196,403 _02133 ECD_02134: ECD_02134 - assembly protein for periplasmic nitrate reductase, at 2,196,400 to 2,196,663 _02134 ECD_02135: ECD_02135 - ferredoxin-type protein, role in electron transfer to periplasmic nitrate reductase NapA, at 2,196,653 to 2,197,147 _02135 ECD_04337: ECD_04337 - hypothetical protein, at 2,197,256 to 2,197,420 _04337 Position (kb) 2193 2194 2195 2196 2197Strain fitness (log2 ratio) -2 -1 0 1 2at 2193.069 kb on - strand, within ECD_02131at 2193.266 kb on + strandat 2193.266 kb on + strandat 2193.267 kb on - strandat 2193.340 kb on + strand, within ECD_02132at 2193.693 kb on + strand, within ECD_02132at 2194.033 kb on - strandat 2194.033 kb on - strandat 2194.033 kb on - strandat 2194.270 kb on - strand, within ECD_02133at 2194.345 kb on - strand, within ECD_02133at 2194.456 kb on - strand, within ECD_02133at 2194.456 kb on - strand, within ECD_02133at 2194.507 kb on - strand, within ECD_02133at 2194.534 kb on - strand, within ECD_02133at 2194.617 kb on + strand, within ECD_02133at 2194.618 kb on - strand, within ECD_02133at 2194.682 kb on + strand, within ECD_02133at 2194.683 kb on - strand, within ECD_02133at 2194.922 kb on - strand, within ECD_02133at 2194.963 kb on + strand, within ECD_02133at 2194.964 kb on - strand, within ECD_02133at 2194.977 kb on + strand, within ECD_02133at 2195.242 kb on - strand, within ECD_02133at 2195.328 kb on + strand, within ECD_02133at 2195.328 kb on + strand, within ECD_02133at 2195.349 kb on - strand, within ECD_02133at 2195.396 kb on - strand, within ECD_02133at 2195.396 kb on - strand, within ECD_02133at 2195.396 kb on - strand, within ECD_02133at 2195.396 kb on - strand, within ECD_02133at 2195.583 kb on + strand, within ECD_02133at 2195.584 kb on - strand, within ECD_02133at 2195.659 kb on + strand, within ECD_02133at 2195.675 kb on - strand, within ECD_02133at 2195.828 kb on - strand, within ECD_02133at 2195.836 kb on + strand, within ECD_02133at 2196.082 kb on + strand, within ECD_02133at 2196.129 kb on + strand, within ECD_02133at 2196.370 kb on + strandat 2196.654 kb on + strandat 2196.669 kb on + strandat 2196.670 kb on - strandat 2196.681 kb on - strandat 2196.739 kb on + strand, within ECD_02135at 2196.740 kb on - strand, within ECD_02135at 2196.781 kb on + strand, within ECD_02135at 2196.803 kb on - strand, within ECD_02135

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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2,193,069 - ECD_02131 0.81 -1.6
2,193,266 + +0.0
2,193,266 + +1.0
2,193,267 - -0.2
2,193,340 + ECD_02132 0.18 +0.5
2,193,693 + ECD_02132 0.69 -0.2
2,194,033 - +0.8
2,194,033 - +1.4
2,194,033 - +2.1
2,194,270 - ECD_02133 0.14 +2.3
2,194,345 - ECD_02133 0.17 +0.6
2,194,456 - ECD_02133 0.22 +0.8
2,194,456 - ECD_02133 0.22 +0.4
2,194,507 - ECD_02133 0.24 -0.9
2,194,534 - ECD_02133 0.25 -0.1
2,194,617 + ECD_02133 0.28 -0.5
2,194,618 - ECD_02133 0.28 +0.1
2,194,682 + ECD_02133 0.31 +0.4
2,194,683 - ECD_02133 0.31 +0.9
2,194,922 - ECD_02133 0.40 +0.2
2,194,963 + ECD_02133 0.42 +0.5
2,194,964 - ECD_02133 0.42 -0.1
2,194,977 + ECD_02133 0.43 -0.9
2,195,242 - ECD_02133 0.53 +0.5
2,195,328 + ECD_02133 0.57 -0.3
2,195,328 + ECD_02133 0.57 +0.0
2,195,349 - ECD_02133 0.58 -0.1
2,195,396 - ECD_02133 0.59 +0.6
2,195,396 - ECD_02133 0.59 +1.8
2,195,396 - ECD_02133 0.59 -0.5
2,195,396 - ECD_02133 0.59 -0.5
2,195,583 + ECD_02133 0.67 +0.5
2,195,584 - ECD_02133 0.67 +0.3
2,195,659 + ECD_02133 0.70 +0.1
2,195,675 - ECD_02133 0.71 +0.9
2,195,828 - ECD_02133 0.77 +0.3
2,195,836 + ECD_02133 0.77 +0.5
2,196,082 + ECD_02133 0.87 +0.7
2,196,129 + ECD_02133 0.89 +0.6
2,196,370 + -2.2
2,196,654 + +0.9
2,196,669 + +0.1
2,196,670 - -0.6
2,196,681 - -0.2
2,196,739 + ECD_02135 0.17 +0.1
2,196,740 - ECD_02135 0.18 +0.8
2,196,781 + ECD_02135 0.26 -1.2
2,196,803 - ECD_02135 0.30 -0.1

Or see this region's nucleotide sequence