Strain Fitness in Escherichia coli BL21 around ECD_01396

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01394 and ECD_04317 are separated by 221 nucleotidesECD_04317 and ECD_01395 are separated by 24 nucleotidesECD_01395 and ECD_01396 are separated by 78 nucleotidesECD_01396 and ECD_01397 are separated by 244 nucleotides ECD_01394: ECD_01394 - uncharacterized protein, at 1,462,897 to 1,463,127 _01394 ECD_04317: ECD_04317 - mRNA interferase toxin of the HicAB toxin-antitoxin system, at 1,463,349 to 1,463,525 _04317 ECD_01395: ECD_01395 - antitoxin for the HicAB toxin-antitoxin system, at 1,463,550 to 1,463,987 _01395 ECD_01396: ECD_01396 - putative DNA-binding transcriptional regulator and putative aminotransferase, at 1,464,066 to 1,465,472 _01396 ECD_01397: ECD_01397 - putative ABC transporter periplasmic binding protein, at 1,465,717 to 1,466,862 _01397 Position (kb) 1464 1465 1466Strain fitness (log2 ratio) -1 0 1at 1463.099 kb on - strand, within ECD_01394at 1463.288 kb on + strandat 1463.325 kb on + strandat 1463.327 kb on + strandat 1463.613 kb on + strand, within ECD_01395at 1464.228 kb on + strand, within ECD_01396at 1465.125 kb on + strand, within ECD_01396at 1465.307 kb on - strand, within ECD_01396at 1466.284 kb on + strand, within ECD_01397

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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1,463,099 - ECD_01394 0.87 -0.2
1,463,288 + +1.5
1,463,325 + -0.6
1,463,327 + -0.7
1,463,613 + ECD_01395 0.14 +0.0
1,464,228 + ECD_01396 0.12 +0.0
1,465,125 + ECD_01396 0.75 +0.0
1,465,307 - ECD_01396 0.88 +1.4
1,466,284 + ECD_01397 0.49 +0.3

Or see this region's nucleotide sequence