Strain Fitness in Escherichia coli BL21 around ECD_00541

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00539 and ECD_00540 are separated by 93 nucleotidesECD_00540 and ECD_00541 are separated by 64 nucleotidesECD_00541 and ECD_00542 are separated by 65 nucleotides ECD_00539: ECD_00539 - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, at 563,440 to 564,093 _00539 ECD_00540: ECD_00540 - DUF419 family protein, at 564,187 to 564,555 _00540 ECD_00541: ECD_00541 - DUF1158 family putative inner membrane protein, at 564,620 to 564,868 _00541 ECD_00542: ECD_00542 - weak gamma-glutamyl:cysteine ligase, at 564,934 to 566,052 _00542 Position (kb) 564 565Strain fitness (log2 ratio) -1 0 1at 563.716 kb on - strand, within ECD_00539at 563.874 kb on + strand, within ECD_00539at 563.874 kb on + strand, within ECD_00539at 563.891 kb on + strand, within ECD_00539at 563.892 kb on - strand, within ECD_00539at 563.949 kb on + strand, within ECD_00539at 563.988 kb on - strand, within ECD_00539at 564.156 kb on - strandat 564.176 kb on - strandat 564.176 kb on - strandat 564.356 kb on - strand, within ECD_00540at 564.436 kb on - strand, within ECD_00540at 564.590 kb on + strandat 564.590 kb on + strandat 564.591 kb on - strandat 564.670 kb on - strand, within ECD_00541at 564.868 kb on + strandat 564.884 kb on - strandat 564.935 kb on + strandat 565.432 kb on + strand, within ECD_00542at 565.456 kb on - strand, within ECD_00542at 565.542 kb on + strand, within ECD_00542at 565.542 kb on + strand, within ECD_00542at 565.542 kb on + strand, within ECD_00542at 565.542 kb on + strand, within ECD_00542at 565.543 kb on - strand, within ECD_00542at 565.544 kb on + strand, within ECD_00542at 565.545 kb on - strand, within ECD_00542at 565.545 kb on - strand, within ECD_00542at 565.607 kb on + strand, within ECD_00542at 565.608 kb on - strand, within ECD_00542at 565.723 kb on + strand, within ECD_00542at 565.805 kb on - strand, within ECD_00542

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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563,716 - ECD_00539 0.42 +0.3
563,874 + ECD_00539 0.66 +0.6
563,874 + ECD_00539 0.66 -0.6
563,891 + ECD_00539 0.69 -0.1
563,892 - ECD_00539 0.69 +0.6
563,949 + ECD_00539 0.78 -0.1
563,988 - ECD_00539 0.84 +0.9
564,156 - +0.8
564,176 - -1.1
564,176 - +0.4
564,356 - ECD_00540 0.46 -0.9
564,436 - ECD_00540 0.67 +0.5
564,590 + -0.2
564,590 + -0.8
564,591 - -0.1
564,670 - ECD_00541 0.20 -0.5
564,868 + -0.0
564,884 - -1.1
564,935 + +0.2
565,432 + ECD_00542 0.45 -0.8
565,456 - ECD_00542 0.47 +0.8
565,542 + ECD_00542 0.54 +0.6
565,542 + ECD_00542 0.54 +1.2
565,542 + ECD_00542 0.54 +0.2
565,542 + ECD_00542 0.54 -0.1
565,543 - ECD_00542 0.54 -1.4
565,544 + ECD_00542 0.55 +0.1
565,545 - ECD_00542 0.55 +0.4
565,545 - ECD_00542 0.55 +1.0
565,607 + ECD_00542 0.60 -0.4
565,608 - ECD_00542 0.60 -0.4
565,723 + ECD_00542 0.71 +0.2
565,805 - ECD_00542 0.78 -0.4

Or see this region's nucleotide sequence