Strain Fitness in Escherichia coli BL21 around ECD_03742

Experiment: Bas51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_r00015 and ECD_r00016 are separated by 186 nucleotidesECD_r00016 and ECD_03742 are separated by 101 nucleotidesECD_03742 and ECD_03743 overlap by 19 nucleotidesECD_03743 and ECD_03744 are separated by 69 nucleotidesECD_03744 and ECD_03745 are separated by 76 nucleotides ECD_r00015: ECD_r00015 - 23S ribosomal RNA, at 3,944,332 to 3,947,235 _r00015 ECD_r00016: ECD_r00016 - 5S ribosomal RNA, at 3,947,422 to 3,947,541 _r00016 ECD_03742: ECD_03742 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 3,947,643 to 3,948,170 _03742 ECD_03743: ECD_03743 - molybdopterin-guanine dinucleotide synthase, at 3,948,152 to 3,948,736 _03743 ECD_03744: ECD_03744 - DUF1040 protein YihD, at 3,948,806 to 3,949,075 _03744 ECD_03745: ECD_03745 - Cpx stress response Thr/Ser protein kinase; MazF antagonist protein, at 3,949,152 to 3,950,138 _03745 Position (kb) 3947 3948 3949Strain fitness (log2 ratio) -2 -1 0 1 2at 3947.634 kb on + strandat 3947.634 kb on + strandat 3947.702 kb on + strand, within ECD_03742at 3947.702 kb on + strand, within ECD_03742at 3947.703 kb on - strand, within ECD_03742at 3947.703 kb on - strand, within ECD_03742at 3947.757 kb on - strand, within ECD_03742at 3947.757 kb on - strand, within ECD_03742at 3947.901 kb on + strand, within ECD_03742at 3947.998 kb on + strand, within ECD_03742at 3947.998 kb on + strand, within ECD_03742at 3947.998 kb on + strand, within ECD_03742at 3947.999 kb on - strand, within ECD_03742at 3947.999 kb on - strand, within ECD_03742at 3947.999 kb on - strand, within ECD_03742at 3948.003 kb on - strand, within ECD_03742at 3948.003 kb on - strand, within ECD_03742at 3948.041 kb on + strand, within ECD_03742at 3948.042 kb on - strand, within ECD_03742at 3948.048 kb on - strand, within ECD_03742at 3948.048 kb on - strand, within ECD_03742at 3948.107 kb on + strand, within ECD_03742at 3948.191 kb on - strandat 3948.214 kb on + strand, within ECD_03743at 3948.260 kb on + strand, within ECD_03743at 3948.260 kb on + strand, within ECD_03743at 3948.261 kb on - strand, within ECD_03743at 3948.291 kb on + strand, within ECD_03743at 3948.334 kb on + strand, within ECD_03743at 3948.411 kb on - strand, within ECD_03743at 3948.556 kb on - strand, within ECD_03743at 3948.607 kb on + strand, within ECD_03743at 3948.607 kb on + strand, within ECD_03743at 3948.607 kb on + strand, within ECD_03743at 3948.608 kb on - strand, within ECD_03743at 3948.669 kb on - strand, within ECD_03743at 3948.717 kb on - strandat 3948.773 kb on - strandat 3948.793 kb on - strandat 3948.793 kb on - strandat 3948.805 kb on + strandat 3948.815 kb on - strandat 3949.066 kb on + strandat 3949.067 kb on - strandat 3949.083 kb on + strandat 3949.157 kb on + strandat 3949.157 kb on + strandat 3949.157 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas51
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3,947,634 + -1.2
3,947,634 + -2.2
3,947,702 + ECD_03742 0.11 -1.1
3,947,702 + ECD_03742 0.11 -0.2
3,947,703 - ECD_03742 0.11 +0.3
3,947,703 - ECD_03742 0.11 -1.6
3,947,757 - ECD_03742 0.22 +1.8
3,947,757 - ECD_03742 0.22 +1.4
3,947,901 + ECD_03742 0.49 +1.6
3,947,998 + ECD_03742 0.67 -0.2
3,947,998 + ECD_03742 0.67 +0.5
3,947,998 + ECD_03742 0.67 +0.2
3,947,999 - ECD_03742 0.67 +0.5
3,947,999 - ECD_03742 0.67 +0.3
3,947,999 - ECD_03742 0.67 +0.3
3,948,003 - ECD_03742 0.68 +0.4
3,948,003 - ECD_03742 0.68 -0.4
3,948,041 + ECD_03742 0.75 +1.6
3,948,042 - ECD_03742 0.76 +0.2
3,948,048 - ECD_03742 0.77 -0.9
3,948,048 - ECD_03742 0.77 +0.5
3,948,107 + ECD_03742 0.88 +1.2
3,948,191 - -0.6
3,948,214 + ECD_03743 0.11 +0.2
3,948,260 + ECD_03743 0.18 +0.7
3,948,260 + ECD_03743 0.18 +0.3
3,948,261 - ECD_03743 0.19 -1.5
3,948,291 + ECD_03743 0.24 -0.3
3,948,334 + ECD_03743 0.31 -1.0
3,948,411 - ECD_03743 0.44 +0.2
3,948,556 - ECD_03743 0.69 -1.1
3,948,607 + ECD_03743 0.78 +1.5
3,948,607 + ECD_03743 0.78 +0.8
3,948,607 + ECD_03743 0.78 -0.6
3,948,608 - ECD_03743 0.78 +0.7
3,948,669 - ECD_03743 0.88 +0.4
3,948,717 - +0.4
3,948,773 - +0.4
3,948,793 - +0.7
3,948,793 - -1.1
3,948,805 + +0.6
3,948,815 - +0.6
3,949,066 + +0.8
3,949,067 - -0.2
3,949,083 + -0.2
3,949,157 + -0.9
3,949,157 + -0.3
3,949,157 + +0.1

Or see this region's nucleotide sequence