Strain Fitness in Escherichia coli BL21 around ECD_r00020

Experiment: Bas51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03883 and ECD_r00020 are separated by 616 nucleotidesECD_r00020 and ECD_t00078 are separated by 85 nucleotidesECD_t00078 and ECD_r00021 are separated by 184 nucleotides ECD_03883: ECD_03883 - IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase, at 4,113,968 to 4,115,557 _03883 ECD_r00020: ECD_r00020 - 16S ribosomal RNA, at 4,116,174 to 4,117,715 _r00020 ECD_t00078: ECD_t00078 - tRNA-Glu, at 4,117,801 to 4,117,876 _t00078 ECD_r00021: ECD_r00021 - 23S ribosomal RNA, at 4,118,061 to 4,120,966 _r00021 Position (kb) 4116 4117 4118Strain fitness (log2 ratio) -1 0 1at 4115.333 kb on + strand, within ECD_03883at 4115.333 kb on + strand, within ECD_03883at 4115.333 kb on + strand, within ECD_03883at 4115.333 kb on + strand, within ECD_03883at 4115.334 kb on - strand, within ECD_03883at 4115.426 kb on + strandat 4115.427 kb on - strandat 4115.560 kb on + strandat 4115.560 kb on + strandat 4115.561 kb on - strandat 4115.580 kb on - strandat 4115.580 kb on - strandat 4115.580 kb on - strandat 4115.583 kb on - strandat 4115.583 kb on - strandat 4115.583 kb on - strandat 4115.583 kb on - strandat 4115.795 kb on + strandat 4115.795 kb on + strandat 4115.877 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas51
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4,115,333 + ECD_03883 0.86 +0.1
4,115,333 + ECD_03883 0.86 -1.0
4,115,333 + ECD_03883 0.86 +0.7
4,115,333 + ECD_03883 0.86 +1.0
4,115,334 - ECD_03883 0.86 +0.6
4,115,426 + -0.5
4,115,427 - -0.0
4,115,560 + -0.4
4,115,560 + +0.1
4,115,561 - +1.0
4,115,580 - -0.5
4,115,580 - -0.7
4,115,580 - -0.6
4,115,583 - +0.5
4,115,583 - -0.2
4,115,583 - -1.0
4,115,583 - -1.1
4,115,795 + -1.2
4,115,795 + -0.3
4,115,877 - -1.2

Or see this region's nucleotide sequence